Animal Cell Culture Techniques PDF Download
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Author: Martin Clynes
Publisher: Springer Science & Business Media
ISBN: 3642804128
Category : Science
Languages : en
Pages : 624
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Book Description
Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.
Author: Martin Clynes
Publisher: Springer Science & Business Media
ISBN: 3642804128
Category : Science
Languages : en
Pages : 624
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Book Description
Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.
Author: Institute of Medicine
Publisher: National Academies Press
ISBN: 0309055822
Category : Science
Languages : en
Pages : 105
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Book Description
The United States is entering an era when, more than ever, the sharing of resources and information might be critical to scientific progress. Every dollar saved by avoiding duplication of efforts and by producing economies of scale will become increasingly important as federal funding enters an era of fiscal restraint. This book focuses on six diverse case studies that share materials or equipment with the scientific community at large: the American Type Culture Collection, the multinational coordinated Arabidopsis thaliana Genome Research Project, the Jackson Laboratory, the Washington Regional Primate Research Center, the Macromolecular Crystallography Resource at the Cornell High-Energy Synchrotron Source, and the Human Genome Center at Lawrence Livermore National Laboratory. The book also identifies common strengths and problems faced in the six cases, and presents a series of recommendations aimed at facilitating resource sharing in biomedical research.
Author: Sara Cohen
Publisher: Springer Science & Business Media
ISBN: 1489913823
Category : Medical
Languages : en
Pages : 197
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Book Description
Vaccination is one of the most efficient and cost effective methods of promoting human health and has been in clinical use for at least 200 years. Nevertheless, infectious diseases continue to constitute a constant threat to the well being of humanity. Common pathogens, once believed to be under control, acquire increased virulence and resistance to drugs, while exotic microorganisms emerged from hidden reservoirs to cause yet incurable diseases in humans. These changes, together with epidemic outbreaks related to political and socio-economic instabilities, increase the needs for the development of new, advanced vaccines. In this volume, devoted to the proceedings of the 39th OHOLO Conference, we present some of the recent strategies for the design and production of novel vaccines. The advent of recombinant DNA technology has stimulated the production of several subunit vaccines. In spite of the obvious advantages to this approach, the limited immuno genicity of many subunit candidates has hindered their development. Strategies to enhance the immunogenicity of subunit vaccines is therefore critical. Several approaches toward this goal, including design of novel adjuvants and delivery systems as well as design of advantageous carriers, are presented here. Among the carriers evaluated here are polypep tides (flagellin, HBV core antigen, J3-galactosidase), attenuated virions (Vaccinia, Sindbis), and nonpathogenic licensed bacteria (Salmonella).
Author: Paul F. Jr. Kruse
Publisher: Elsevier
ISBN: 0323142079
Category : Science
Languages : en
Pages : 897
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Book Description
Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.
Author: Erik Lycke
Publisher: Butterworth-Heinemann
ISBN: 148319194X
Category : Medical
Languages : en
Pages : 384
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Book Description
Textbook of Medical Virology presents a critical review of general principles in the field of medical virology. It discusses the description and molecular structures of virus. It addresses the morphology and classifications of viruses. It also demonstrates the principal aspects of virus particle structure. Some of the topics covered in the book are the symmetrical arrangements of viruses; introduction to different families of animal viruses; biochemistry of virus particles; the immunological properties and biological activities of viral gene products; description of enzymatic activities of viruses; and haemagglutination, cell fusion, and haemolysis of viruses. The description and characteristics of viral antigens are covered. The identification and propagation of viruses in tissue and cell cultures are discussed. An in-depth analysis of the principles of virus replication is provided. A study of the morphogenesis of virions is also presented. A chapter is devoted to virus-induced changes of cell structures and functions. The book can provide useful information to virologists, microbiologists, students, and researchers.
Author: Just M. Vlak
Publisher: Springer Science & Business Media
ISBN: 0306468506
Category : Science
Languages : en
Pages : 303
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Book Description
A comprehensive reference work covering the key issues in insect cell cultures, this text includes 30 review papers on such topics as: cell lines (development, characterisation, physiology, cultivation and medium design); viruses (virus-cell interactions, replication, recombinant construction, infection kinetics, post-translational modification and passage effects); engineering (shear, bioreactors including perfusion, immobilisation, scale-up and modelling, downstream processing); applications; and economics and regulatory aspects.; This text should be useful for cell biologists, biochemists, molecular biologists, virologists, immunologists and other basic and applied disciplines related to cell culture engineering, both academic and industrial.
Author: S. Nandi
Publisher: New India Publishing
ISBN: 9789380235059
Category : Science
Languages : en
Pages : 178
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Book Description
The book is written in a very simple and lucid manner so that everybody can read and understand it very easily. The book is useful for scientists, teachers, students, officers, diagnosticians and laboratory technicians as cell culture has become an essential and indispensable tool in many branches of life sciences and application of cell culture is getting increased exponentially day by day in various fields of biological and medical research arena. This book will provide detailed information on all the aspects of the cell culture starting from establishment of a cell culture laboratory, primary culture, secondary culture, media filtration, collection, preservation and dispatch of samples for diagnosis of viral diseases, cell line authentication and characterization, contamination and curing, cryopreservation of cells and revival of cells besides description on ELISA, SNT, virus titration etc. In my opinion, this book will be extremely useful to the persons who are directly and indirectly involved in cell culture work for various biological experiments. Finally, students and examinees can enrich their knowledge on cell culture from the book and can face any challenge easily and confidently. s on the latest developments on biotechnological approaches for fish disease diagnostic, infection and immunity of brood carps, cryoconservation of fishes, probiotics and nanotechnology in aquaculture are of paramount interest, in addition to information on prawn aquaculture, ornamental fish farming and trade. Information on various software and their application for exploratory data analysis and data mining leading to knowledge discovery and visualization is the main attraction of the book. Another important feature of the book is that one can find appropriate as well as illustrated examples exclusively with fisheries data. The statistics section includes biometrical and qualitative techniques in genetics and selective breeding of fish, besides fundamental statistical test, design of experiments and sampling methods for planning of experiments and survey in fisheries and aquaculture research. The book also includes econometric approach for technical efficiency estimation and input-output analysis, project evaluation, and impact assessment, linear programming, market intelligence, fisheries legislation, policy and IPR issues all of which are new in the field of fisheries and aquaculture.
Author: E. N. Willmer
Publisher: Elsevier
ISBN: 1483277232
Category : Science
Languages : en
Pages : 843
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Book Description
Cells and Tissues in Culture: Methods, Biology, and Physiology, Volume 3 focuses on the applications of the methods of tissue culture to various fields of investigation, including virology, immunology, and preventive medicine. The selection first offers information on molecular organization of cells and tissues in culture and tissue culture in radiobiology. Topics include cellular organization at the molecular level, fibrogenesis in tissue culture, effect of radiation on the growth of isolated cells, and irradiation of the selected parts of the cell. The publication then considers the effects of invading organisms on cells and tissues in culture and cell, tissue, and organ cultures in virus research. The book elaborates on antibody production in tissue culture and tissue culture in pharmacology. Discussions focus on early attempts at in vitro studies, tissue culture in the study of pharmacologically active agents, and methods of assessment of drug activity. The text also reviews invertebrate tissue and organ culture in cell research; introduction and methods employed in plant tissue culture; and growth, differentiation and organogenesis in plant tissue and organ cultures. The selection is a vital source of data for readers interested in the culture of cells and tissues.
Author: R. Ian Freshney
Publisher: Wiley-Liss
ISBN: 9780471589662
Category : Science
Languages : en
Pages : 510
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Book Description
This masterful third edition of Freshney's Culture of Animal Cells updates and considerably expands the scope of its predecessor and still enables both the novice and the experiences researcher to apply the basic and more sophisticated techniques of tissue culture. New Topics covered include: the use of molecular techniques in cell culture, such as DNA fingerprinting, fluorescence in situ hybridization, and chromosome painting cell interactions in cell culture new methods for separating cells new or refined methods for accessing cytotoxicity, viability, and mutagenicity experimental details for culture of specialized cells types not covered in previous editions new or refined techniques for visualizing clues, including time-lapse photography and confocal microscopy The revised and expanded third edition offers the following features: over 350 new reference to the primary literature an international list of cell banks an international listing of reagants and commercial supplies a subject index a glossary Also available: 0471169021 Culture of Animal Cells: A Multimedia Guide CD-ROM $150 est. From the reviews: "I strongly recommend this volume for any laboratory wishing to culture mammalian cells" - Biotechnology "It is not very often that it is possible to say of a book, 'I don't know how I managed without it previously.' Here is such a book" - Cell Biology International Reports
Author: Mohamed Al-Rubeai
Publisher: Springer
ISBN: 3319103202
Category : Medical
Languages : en
Pages : 766
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Book Description
Animal cells are the preferred “cell factories” for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libraries.
