Synthetic Biology, Genetics and Advanced Topics PDF Download
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Author: Edenilson Brandl
Publisher: Edenilson Brandl
ISBN:
Category : Health & Fitness
Languages : en
Pages : 515
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Book Description
Synthetic Biology is a scientific discipline that has the potential to revolutionize the way we understand and manipulate life. Combining knowledge from biology, genetics, engineering and computing, Synthetic Biology opens up new possibilities for creating personalized biological systems, with applications ranging from regenerative medicine to the production of biofuels. In this book, we will explore the principles, techniques, and applications of synthetic biology, with the aim of providing a comprehensive and up-to-date overview of this ever-evolving field. Throughout the chapters, we will delve into the molecular foundations of Synthetic Biology, exploring the biological components and processes involved in building synthetic biological systems. We will present several applications of Synthetic Biology in different sectors, highlighting its role in the pharmaceutical industry, in the production of biofuels, in the development of personalized vaccines and in regenerative medicine. We will see how the manipulation of synthetic stem cells can revolutionize the area of tissue regeneration and how metabolic engineering allows us to design microorganisms capable of producing valuable compounds. Bioinformatics plays a crucial role in Synthetic Biology, and we will dedicate a chapter to exploring how the analysis of genomic data and the use of bioinformatics algorithms contribute to the advancement of this discipline. In addition, we will cover topics such as genome editing using CRISPR technology, synthetic protein engineering, and directed evolution in the laboratory. With the advancement of technology, it has become possible to manufacture living tissue in the laboratory, and we will discuss biofabrication and 3D printing of living tissue, as well as the use of microfluidics in the manipulation and analysis of synthetic biological systems on a microscopic scale. We will also address ethical and biosafety issues related to Synthetic Biology, recognizing the importance of a responsible approach in all its applications. This book is intended for students, researchers, and practitioners interested in exploring the frontiers of synthetic biology. We hope it will be an inspiring source of knowledge, awakening the curiosity and innovative spirit of all readers. We invite you to embark on this exciting journey through Synthetic Biology, in search of a deeper understanding of life and its transformative possibilities.
Author: Richard M. Twyman
Publisher:
ISBN:
Category : Molecular biology
Languages : en
Pages : 0
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Book Description
Author: Khalid Z. Masoodi
Publisher: Academic Press
ISBN: 012824450X
Category : Science
Languages : en
Pages : 220
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Book Description
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Author: Heather B. Miller
Publisher: Academic Press
ISBN: 0123855454
Category : Science
Languages : en
Pages : 227
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Book Description
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author: Alfred Pühler
Publisher: Springer Science & Business Media
ISBN: 3642693059
Category : Science
Languages : en
Pages : 353
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Book Description
The development of powerful new techniques and refmements of tech niques in molecular genetics in recent years, and the surge in interest in biotechnology based on genetic methods, have heralded a new golden age in molecular genetics, and stimulated in diverse disciplines much interest in the technologies themselves and their potential uses in basic and applied biomedical sciences. Although some excellent specialist laboratory manuals (especially the Cold Spring Harbor Laboratory manuals by I. H. Miller; R. W. Davies et al. ; and T. Maniatis et al. ) on certain chapters of molecular genetics exist, no general text that covers a broad spectrum of the sub ject has thus far been published. The purpose of this manual is to pre sent most, though of necessity not all of the important methods of molecular genetics, in a series of simple experiments, many of which can be readily accomplished by the microbiologist, biochemist or biotechnologist that has had only limited exposure to genetics. The remainder of the experiments require either greater familiarity with the subject, or guidance by someone with such experience. The book should, therefore, not only enable individuals to acquire new proce dures for ongoing projects, but also serve as a basis for the teaching of molecular genetic techniques in formal predoctoral and postdoctoral laboratory courses.
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Book Description
Author: Wolfgang Schulz
Publisher: Springer Science & Business Media
ISBN: 1402031858
Category : Medical
Languages : en
Pages : 519
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Book Description
Cancer research is now an interdisciplinary effort requiring a basic knowledge of commonly used terms, facts, issues, and concepts. This interdisciplinary book meets this need, providing an authoritative overview to the field. It presents many of the molecules and mechanisms generally important in human cancers and examines a broad, but exemplary, selection of cancers. In addition, cancer research has now reached a critical stage, in which the accumulated knowledge on molecular mechanisms is gradually translated into improved prevention, diagnosis, and treatment. This book summarizes the state, pitfalls, and potential of these efforts.
Author: John Wilson
Publisher: Garland Science
ISBN: 1317497279
Category : Science
Languages : en
Pages : 985
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Book Description
The Problems Book helps students appreciate the ways in which experiments and simple calculations can lead to an understanding of how cells work by introducing the experimental foundation of cell and molecular biology. Each chapter reviews key terms, tests for understanding basic concepts, and poses research-based problems. The Problems Book has be
Author: Swati Agarwal
Publisher: I K International Pvt Ltd
ISBN: 9385909967
Category : Medical
Languages : en
Pages : 166
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Book Description
This book provides detailed information on various instruments, techniques and experiment protocols of biochemistry and molecular biology. It deals with basic as well as advanced information and in-depth methodology in simple language to help students and professionals to perform experiments with ease. This book not only clears the practical concepts of Biochemistry and Molecular Biology at undergraduate and post-graduation levels, but also helps to pass the Ph.D. course work exam conducted by various universities. This book will develop research aptitude to clear the NET examination. This manual gives a comprehensive idea about the various instruments, their working, troubleshooting and their applications. It provides a wide spectrum of 14 chapters covering basic as well as advanced techniques and instrumentation, viz., Gas Chromatography (GC), Mass Spectrometry (MS), Scanning Electron Microscope (SEM), X-Ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR) with detailed protocols. Most of the experiments can be easily performed in the laboratory having basic facilities. Historical background, experiment nature, its principle, step-by-step procedure with diagrammatic representation and important precautions are given in the beginning of each experiment.
Author:
Publisher:
ISBN: 9780471503385
Category : Molecular biology
Languages : en
Pages :
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Book Description
Author: Jennifer Hackett
Publisher: Independently Published
ISBN: 9781095827925
Category :
Languages : en
Pages : 162
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Book Description
This workbook is a companion to the introductory college-level textbook, Molecular Biology: Concepts for Inquiry. The workbook contains inquiry explorations that that have been designed for use in the classroom, but could also be used for individual study. It is appropriate for college courses and high school courses taught at the college level. CLASSROOM ACTIVITIES: Students explore evidence through logic to construct an understanding of concepts and eliminate misconceptions. Students elaborate on their understanding by applying it to new situations. These activities are intended to be conducted in a classroom where an instructor periodically guides student thinking in small groups and leads class discussions of key concepts following activities. Inquiry activities include: introductory biochemistry, how proteins contribute to modes of inheritance, the structure and function of fluorescent proteins, the conceptual basis of PCR, the function of restriction enzymes and their use in engineering, the design of the mutagenesis of fluorescent proteins through Gibson assembly, analysis of an iGEM device, the design of a Golden Gate assembly of gene parts, epigenetic inheritance in imprinted diseases, analysis of the genetics of cancer (childhood vs. adult; inherited predisposition vs. sporadic), genome instability at telomeres, evaluation of next-generation DNA sequencing strategies, and the design of a CRISPR RNA to cure a genetic disease. A subset of the class activities focuses on pre- or post-experiment analyses that could either stand alone or could be used as a conceptual framework around which experiments could be conducted. Suggested experiments and other supporting materials are provided on the author's website, https://hackettmolecularbiology.blogspot.com/. Because the paperback workbook is printed in black and white to reduce cost to the student, color images for the one activity (fluorescent proteins) that would be best in color are also provided on the author's website and the Kindle eBook includes these images in color. CLASSROOM DISCUSSION QUESTIONS: These open-ended questions serve as the basis for class discussions following Molecular Biology: Concepts for Inquiry textbook reading assignments. These readings and discussions substitute for most direct lecture in explaining concepts and they are also accompanied by online self-assessment reading comprehension quizzes. The author will distribute quiz questions to instructors for their own editing and distribution or individuals may take the author's version of quizzes. UNIT SELF-ASSESSMENTS: Students can assess their overall conceptual understanding through these assessment questions and the answers that are provided. APPENDICES AND REFERENCE MATERIALS: Self-assessment answers, guidelines for basic molecular biology laboratory techniques including PCR and restriction digests, explanations of the function of bacterial and phage promoters commonly used in engineering, list of commonly-used restriction enzymes, structures of amino acids, genetic code, periodic table, and other references. AUTHOR RECOMMENDATIONS: 1) Because it is intended that students will write in this workbook, purchasing the paperback version is recommended. The Kindle eBook is available as a free MatchBook after purchase of the paperback. 2) If you are studying on your own instead of using this workbook as part of a class, you might consider purchasing the teacher's guide, Molecular Biology Concepts for Inquiry: A Guide to Inquiry. The teacher's guide, available June-July 2019, will contain the contents of this workbook, answers, commentary, and notes to the teacher about how to teach Molecular Biology through Inquiry and suggestions on how to guide students in the classroom.
