Author: Taosheng Chen
Publisher: CRC Press
ISBN: 1420070517
Category : Medical
Languages : en
Pages : 291
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Book Description
The development of suitable assays, the integration of appropriate technology, and the effective management of the essential infrastructure are all critical to the success of any high-throughput screening (HTS) endeavor. However, few scientists have the multidisciplinary experience needed to control all aspects of an HTS drug discovery project. A P
Author: Joshua A Bittker
Publisher: Royal Society of Chemistry
ISBN: 1782626778
Category : Medical
Languages : en
Pages : 396
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Book Description
High throughput screening remains a key part of early stage drug and tool compound discovery, and methods and technologies have seen many fundamental improvements and innovations over the past 20 years. This comprehensive book provides a historical survey of the field up to the current state-of-the-art. In addition to the specific methods, this book also considers cultural and organizational questions that represent opportunities for future success. Following thought-provoking foreword and introduction from Professor Stuart Schreiber and the editors, chapters from leading experts across academia and industry cover initial considerations for screening, methods appropriate for different goals in small molecule discovery, newer technologies that provide alternative approaches to traditional miniaturization procedures, and practical aspects such as cost and resourcing. Within the context of their historical development, authors explain common pitfalls and their solutions. This book will serve as both a practical reference and a thoughtful guide to the philosophy underlying technological change in such a fast-moving area for postgraduates and researchers in academia and industry, particularly in the areas of chemical biology, pharmacology, structural biology and assay development.
Author: Mark Wigglesworth
Publisher:
ISBN: 9781536172539
Category : Technology & Engineering
Languages : en
Pages : 362
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Book Description
"High Throughput Screening (HTS) is one of several hit identification approaches that are part of a developing and evolving toolbox for the discovery of pharmaceutical start points. HTS remains one of the most successful approaches, and therefore an important foundation of drug discovery. In High Throughput Screening: Methods, Techniques and Applications, leading industrial and academic experts in screening and drug discovery explain key technologies and methods while demonstrating how they can be applied to successful hit identification. Describing both traditional and emerging methods in detail, this book provides an overview of these methods to the reader that will serve both those new to the field and expert scientists alike. High Throughput Screening: Methods, Techniques and Applications provides readers with an outline of key elements in the areas of assay development, detailed descriptions of a range of both biochemical and cell-based screening methodologies and strategies, as well as highlighting important steps in data analysis. By describing the basic principles of methods commonly used in HTS, High Throughput Screening: Methods, Techniques and Applications provides an illuminating introduction to HTS, capturing established good practice within the field, thereby imparting both the industrial and academic researcher with the knowledge required to work effectively in both today's and the hit identification laboratories of the future"--
Author: Steven A. Haney
Publisher: John Wiley & Sons
ISBN: 0470624566
Category : Science
Languages : en
Pages : 348
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Book Description
Using a collaborative and interdisciplinary author base with experience in the pharmaceutical industry and academia, this book is a practical resource for high content (HC) techniques. • Instructs readers on the fundamentals of high content screening (HCS) techniques • Focuses on practical and widely-used techniques like image processing and multiparametric assays • Breaks down HCS into individual modules for training and connects them at the end • Includes a tutorial chapter that works through sample HCS assays, glossary, and detailed appendices
Author: William P. Janzen
Publisher: Springer Science & Business Media
ISBN: 1592591809
Category : Medical
Languages : en
Pages : 269
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Book Description
In High Throughput Screening, leading scientists and researchers expert in molecular discovery explain the diverse technologies and key techniques used in HTS and demonstrate how they can be applied generically. Writing to create precisely the introductory guidebook they wish had been available when they started in HTS, these expert seasoned authors illuminate the HTS process with richly detailed tutorials on the biological techniques involved, the management of compound libraries, and the automation and engineering approaches needed. Extensive discussions provide readers with all those key elements of pharmacology, molecular biology, enzymology, and biochemistry that will ensure the identification of suitable targets and screens, and detail the technology necessary to mine millions of data points for meaningful knowledge.
Author: Lisa K. Minor
Publisher: CRC Press
ISBN: 1420015702
Category : Medical
Languages : en
Pages : 490
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Book Description
The Handbook of Assay Development in Drug Discovery describes all the tools currently available for performing various assay techniques. Featuring troubleshooting advice for common problems from experienced assay developers, the vendor community, and scientists in the pharmaceutical industry, the book presents descriptions of methods, laboratory guidelines and protocols used to perform such methods, specific examples of each assay system, and troubleshooting tools. Designed as a guide to running an assay from start to finish, this is an ideal bench top companion for scientists involved in drug discovery screening, lead profiling, therapeutic target evaluation, and assay development and implementation.
Author: Sheraz Gul
Publisher:
ISBN: 9781849731393
Category : Science
Languages : en
Pages : 256
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Book Description
This book is one of a three-volume set that provides theoretical, practical and troubleshooting guidelines on all aspects of the pre-clinical Drug Discovery process. There is particular focus on the design, development and role of screening assays in Drug Discovery, including new developments and future trends, the main assay types utilized in screening campaigns, and the methods utilized in enabling the progression of ""Hits"" to ""Leads"" and ""Candidates"" The books will fill current gaps in the literature such as primary, stem cell and other novel assay methods. Other relatively new research areas included are siRNA, high content screening, protein-protein interactions, pathway assays, non micro-titre plate based assays, label free assays, IT tools, approaches to identify biopharmaceuticals, emerging models and future trends in Drug Discovery.
Author: Ge Wu
Publisher: John Wiley & Sons
ISBN: 0470583118
Category : Science
Languages : en
Pages : 452
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Book Description
Essential principles and practice of assay development The first comprehensive, integrated treatment of the subject, Assay Development: Fundamentals and Practices covers the essentials and techniques involved in carrying out an assay project in either a biotechnology/drug discovery setting or a platform setting. Rather than attempting comprehensive coverage of all assay development technologies, the book introduces the most widely used assay development technologies and illustrates the art of assay development through a few commonly encountered biological targets in assay development (e.g., proteases, kinases, ion channels, and G protein-coupled receptors). Just enough biological background for these biological targets is provided so that the reader can follow the logics of assay development. Chapters discuss: The basics of assay development, including foundational concepts and applications Commonly used instrumental methods for both biochemical assays and cell-based assays Assay strategies for protein binding and enzymatic activity Cell-based assays High-throughput screening An in-depth study of the now popular Caliper's off-chip kinase assay provides an instructive, real-world example of the assay development process.
Author: Jörg Hüser
Publisher: John Wiley & Sons
ISBN: 3527609369
Category : Science
Languages : en
Pages : 362
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Book Description
Backed by leading authorities, this is a professional guide to successful compound screening in pharmaceutical research and chemical biology, including the chemoinformatic tools needed for correct data evaluation. Chapter authors from leading pharmaceutical companies as well as from Harvard University discuss such factors as chemical genetics, binding, cell-based and biochemical assays, the efficient use of compound libraries and data mining using cell-based assay results. For both academics and professionals in the pharma and biotech industries working on small molecule screening.
Author: Daria Mochly-Rosen
Publisher: Springer Nature
ISBN: 3031347242
Category : Medical
Languages : en
Pages : 276
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Book Description
"A lot of hard-won knowledge is laid out here in a brief but informative way. Every topic is well referenced, with citations from both the primary literature and relevant resources from the internet." Review of first edition from Nature Chemical Biology Written by the founders of the SPARK program at Stanford University, this book is a practical guide designed for professors, students and clinicians at academic research institutions who are interested in learning more about the drug development process and how to start transforming their basic research discoveries into novel drugs. Often many potentially transformative basic science discoveries are not pursued because they are deemed ‘too early’ to attract industry interest. This comprehensive book lays out simple, relatively cost-effective things that academic researchers can do to advance their findings to the point that they can be tested in the clinic or attract more industry interest. Each chapter broadly discusses an important topic in drug development, from discovery, optimization and preclinical studies through clinical trial design, regulatory issues and marketing assessments. After the practical overview provided here, the reader is encouraged to consult more detailed texts on specific topics of interest. The SPARK model has been adopted in over 60 institutions on six continents, and the program has been honored with multiple awards including the 2020 Xconomy Award for Ecosystem Development, the 2020 Cures Within Reach Award for Patient Impact Research, and the 2022 California Life Sciences Pantheon Award for Academia, Non-Profits, & Research. The new edition updates every chapter with the latest developments since the 2014 publication of the first edition.
