Author: Mohammed KhanPublisher:
ISBN:
Category : Dielectrophoresis
Languages : en
Pages :
Book Description
Separation of pure population of cells from complex and heterogeneous cell mixtures has immense application on therapeutics and diagnostics of diseases. Chinese hamster ovary (CHO) cell in its viable form is the most widely used mammalian cell line for commercial production of therapeutic protein. On the other hand, Circulating tumor cells (CTCs) are proven elements to have significant prognostic, diagnostic, and clinical values in early-stage cancer detection. Thus, separation of nonviable CHO cells from suspending medium is critical in biopharmaceutical sectors and isolation of CTCs from peripheral blood for proper drug innovation in medical sectors. Passive microfluidic techniques, which can use the geometry to utilize intrinsic fluidic forces to separate different sized cells, are getting attention as a high-throughput microchip technique. Despite extensive performance to separate different size cells, this technique suffers when similarity of size between cells of specific types of CTCs and WBCs or viable and non-viable CHO cells are present. On the other hand, an active technique, i.e., dielectrophoresis, lacks the desired high throughput. Here, two different hybrid microfluidic techniques, one with Deterministic Lateral Displacement (DLD) and another with inertial microfluidics, are tested with CTC and CHO. First, we demonstrated that a DLD device could be combined with a frequency-based AC electric field to perform high-resolution continuous separation of nonviable CHO cells from the viable cells. This coupled DLD-DEP device's behavior is further investigated by employing numerical simulation to check the effect of geometrical parameters of the DLD arrays, velocities of the flow field, and required applied voltages. In the following work, we proposed a hybrid technique that combines inertia microfluidics and dielectrophoresis in order to separate CTCs from overlapping size WBCs through the use of a sheath flow. The cell trajectories along with fluid fields are modeled to investigate the effects of different applied electrical voltages and Reynold numbers on separation characteristics. This technique could be exploited to design a microchip for continuous separation for any cell beads, controlled simply by adjusting the external field frequency.
