Three-Dimensional Confocal Microscopy: Volume Investigation of Biological Specimens PDF Download
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Author:
Publisher: Academic Press
ISBN: 0323138969
Category : Science
Languages : en
Pages : 507
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Book Description
The integration of confocal microscopy and volume investigation has led to an unprecedented ability to examine spatial relationships between cellular structure and function. The goal of this book is to familiarize the reader with these new technologies and to demonstrate their applicability to a wide range of biological and clinical problems. Volume investigation Three-dimensional reconstruction Fluroescent probe design Biological applications of confocal microscopy, including calcium imaging, receptor movement, and diagnostic pathology Confocal data display and analysis Twenty-eight pages of color
Author:
Publisher: Academic Press
ISBN: 0323138969
Category : Science
Languages : en
Pages : 507
Get Book
Book Description
The integration of confocal microscopy and volume investigation has led to an unprecedented ability to examine spatial relationships between cellular structure and function. The goal of this book is to familiarize the reader with these new technologies and to demonstrate their applicability to a wide range of biological and clinical problems. Volume investigation Three-dimensional reconstruction Fluroescent probe design Biological applications of confocal microscopy, including calcium imaging, receptor movement, and diagnostic pathology Confocal data display and analysis Twenty-eight pages of color
Author:
Publisher: Academic Press
ISBN: 0080925359
Category : Science
Languages : en
Pages : 521
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Book Description
This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Includes detailed protocols accessible to new users Details how to set up and run a "Confocal Microscope Core Facility" Contains over 170 figures
Author: James Pawley
Publisher: Springer Science & Business Media
ISBN: 1475753489
Category : Science
Languages : en
Pages : 639
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Book Description
This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.
Author:
Publisher: Academic Press
ISBN: 0128170190
Category : Science
Languages : en
Pages : 303
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Book Description
Three-Dimensional Electron Microscopy, Volume 152 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters focusing on FIB-SEM of mouse nervous tissue: fast and slow sample preparation, Serial-section electron microscopy using ATUM - Automated Tape collecting Ultra-Microtome, Software for automated acquisition of electron tomography tilt series, Scanning electron tomography of biological samples embedded in plastic, Cryo-STEM tomography for Biology, CryoCARE: Content-aware denoising of cryo-EM images and tomograms using artificial neural networks, Expedited large-volume 3-D SEM workflows for comparative vertebrate microanatomical imaging, and many other interesting topics. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Includes the latest information on the Three-Dimensional Electron Microscopy technique
Author: Elli Kohen
Publisher: World Scientific
ISBN: 178326215X
Category : Medical
Languages : en
Pages : 736
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Book Description
This unique book provides a comprehensive description of fluorescence probes and the methodology for the study and diagnostics of oncology. The material is drawn directly from the work of pioneer researchers in cell biology and pathology, and offers a perspective of their most crucial investigations and lifetime experiences; it also opens new horizons on future developments in fundamental methods and diagnostics relevant to cellular physiopathology. Researchers in cell pathology have contributed a broad range of spectral and fluorescence images which most appropriately supplement the information derived from Virchow style microscope slides (these still remain valid after more than 150 years, and a considerable body of knowledge and interpretation can be built around them). The text contains about 100 colour pictures, adding great value to the book. Contents:The Nature of LightIntroduction to PhotophysicsBasic Approaches to the Experimental Study of Chemical and Biological LuminescenceMethods and InstrumentationNew Methods. Theoretical Basis and Potential Applications to BiologyFluorescent ProbesApplications of Fluorescence Techniques to Study Biological Processes in Normal and Pathological CellsCell Physiopathology Readership: Students and researchers in oncology, the life sciences, biology and optics. Keywords:Chemiluminescence;Bioluminescence;Green Fluorescent Protein (GFP);Microspectrofluorimetry;Fluorescence Resonance Energy Transfer (FRET);Fluorescence Lifetime Imaging (FLIM);Fluorescence In Situ Hybridization (FISH);Multicolor Spectral Karyotyping (SKY);Fourier Interferometric Spectral Imaging;Fluorescence Imaging;Fluorescence Probe;Oncology;Cell Biology;Pathology;Diagnostics
Author: M. H. F. Wilkinson
Publisher: John Wiley & Sons
ISBN: 9780471974406
Category : Science
Languages : en
Pages : 588
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Book Description
Providing specific knowledge in the theory of image analysis, optics, fluorescence, and imaging devices in biomedical laboratories, this timely and indispensable volume focuses on the theory and applications of detection, morphometry, and motility measurement techniques applied to bacteria, fungi, yeasts and protozoa.
Author: Robert L. Price
Publisher: Springer Science & Business Media
ISBN: 0387781757
Category : Science
Languages : en
Pages : 302
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Book Description
Most researchers agree that biological confocal microscopy was jump-started by the confocal design first published by White and Amos in 1985 in the Journal of Cell Biology. As a result, this remains a relatively young field. Yet the use of the technique has grown phenomenally since those early efforts, with new users joining the ranks daily. The publication of Basic Confocal Microscopy reflects the burgeoning need to train new students, technologists, and faculty wishing to use confocal microscopy in their research. A direct outgrowth of the authors’ five-day intensive course in the subject begun in 2005, this book covers the basics and includes all the information required to design, implement, and interpret the results of, biological experiments based on confocal microscopy. Concise yet comprehensive, the volume begins by covering the core issues of fluorescence, specimen preparation and labeling, before moving on to address the analog-to-digital conversion of specimen data gathered using confocal microscopy. Subsequent chapters detail the practicalities of operating confocal microscopes, providing all the information necessary to begin practicing confocal microscopy as well as optimizing the material obtained. The final block of chapters examine 3-dimensional analysis and the reconstruction of data sets, outline some of the ethical considerations in confocal imaging, and then supply a number of resources that the authors have found useful in their own work. Once readers have mastered the information this book presents, the resources found in its pages will be an excellent guide to continued learning about the more advanced forms of confocal microscopy.
Author: Douglas J. Taatjes
Publisher: Springer Science & Business Media
ISBN: 1592599931
Category : Medical
Languages : en
Pages : 505
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Book Description
A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.
Author: Jayaram K. Udupa
Publisher: CRC Press
ISBN: 1351470302
Category : Law
Languages : en
Pages : 362
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Book Description
This book provides a quick and systematic presentation of the principles of biomedical visualization and three-dimensional (3D) imaging. Topics discussed include basic principles and algorithms, surgical planning, neurosurgery, orthopedics, prosthesis design, brain imaging, cardio-pulmonary structure analysis and the assessment of clinical efficacy. Students, scientists, researchers, and radiologists will find 3D Imaging in Medicine a valuable source of information for a variety of actual and potential clinical applications for 3-D imaging.
Author:
Publisher: Elsevier
ISBN: 0080496652
Category : Medical
Languages : en
Pages : 696
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Book Description
This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
