The Role of Water in the Control of Enzyme Functionality at Interfaces PDF Download
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Author: Chetan S. Rao
Publisher:
ISBN:
Category :
Languages : en
Pages : 334
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Book Description
Author: Chetan S. Rao
Publisher:
ISBN:
Category :
Languages : en
Pages : 334
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Book Description
Author: Munishwar N. Gupta
Publisher: Birkhäuser
ISBN: 3034884729
Category : Science
Languages : en
Pages : 229
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Book Description
Extending the range of enzymatic catalysis by using non-aqueous media has now developed into a powerful approach in biochemistry and biotechnology. One peculiar feature which distinguishes it from the conventional enzymology (carried out in aqueous buffers) is that the awareness of different parameters that control and influence the behaviour of enzymes in such environments has emerged rather slowly. Science is about being able to repeat what somebody else has done. Absence of knowledge about such well-defined parameters/fac tors has sometimes made some workers rather cautious and diffident about using this approach in their laboratories. But for this, non-aqueous enzymol ogy would be more widely practised. It is these thoughts that made me feel that the availability of some well-defined protocols for various applications invol ving enzymes in non-aqueous environments would further catalyze the growth of this area. Hence this book, in which each chapter has some protocols in a specific area. The protocols are preceded by brief background material. The early chapters, which are of general importance, concern control of water ac tivity and stabilization via immobilization. Some subsequent chapters provide the protocols for transformations involving lipids and carbohydrates, peptide synthesis, and preparation of chiral compounds. The disproportionate focus on lipases is not a coincidence; this class of enzymes has been used more often than others in non-aqueous enzymology.
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Book Description
Author: E. Anibal Disalvo
Publisher: Springer
ISBN: 3319190601
Category : Science
Languages : en
Pages : 295
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Book Description
This book is about the importance of water in determining the structure, stability and responsive behavior of biological membranes. Water confers to lipid membranes unique features in terms of surface and mechanical properties. The analysis of the hydration forces, plasticiser effects, controlled hydration, formation of microdomains of confined water suggests that water is an active constituent in a water-lipid system. The chapters describe water organization at the lipid membrane–water interphase, the water penetration, the long range water structure in the presence of lipid membranes by means of X-ray and neutron scattering, general polarization, fluorescent probes, ATR-FTIR and near infrared spectroscopies, piezo electric methods, computer simulation and surface thermodynamics. Permeation, percolation, osmotic stress, polarization, protrusion, sorption, hydrophobicity, density fluctuations are treated in detail in self-assembled bilayers. Studies in lipid monolayers show the correlation of surface pressure with water activity and its role in peptide and enzyme interactions. The book concludes with a discussion on anhydrobiosis and the effect of water replacement in microdomains and its consequence for cell function. New definitions of lipid/water interphases consider water not only as a structural-making solvent but as a mediator in signalling metabolic activity, modulating protein insertion and enzymatic activity, triggering oscillatory reactions and functioning of membrane bound receptors. Since these effects occur at the molecular level, membrane hydration appears fundamental to understand the behavior of nano systems and confined environments mimicking biological systems. These insights in structural, thermodynamical and mechanical water properties give a base for new paradigms in membrane structure and function for those interested in biophysics, physical chemistry, biology, bio and nano medicine, biochemistry, biotechnology and nano sciences searching for biotechnological inputs in human health, food industry, plant growing and energy conversion.
Author: Thomas Heimburg
Publisher: John Wiley & Sons
ISBN: 3527611606
Category : Science
Languages : en
Pages : 378
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Book Description
An overview of recent experimental and theoretical developments in the field of the physics of membranes, including new insights from the past decade. The author uses classical thermal physics and physical chemistry to explain our current understanding of the membrane. He looks at domain and 'raft' formation, and discusses it in the context of thermal fluctuations that express themselves in heat capacity and elastic constants. Further topics are lipid-protein interactions, protein binding, and the effect of sterols and anesthetics. Many seemingly unrelated properties of membranes are shown to be intimately intertwined, leading for instance to a coupling between membrane state, domain formation and vesicular shape. This also applies to non-equilibrium phenomena like the propagation of density pulses during nerve activity. Also included is a discussion of the application of computer simulations on membranes. For both students and researchers of biophysics, biochemistry, physical chemistry, and soft matter physics.
Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 994
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Book Description
Author: R. Roger Ruan
Publisher: CRC Press
ISBN: 9781566765893
Category : Technology & Engineering
Languages : en
Pages : 316
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Book Description
This book is the first book on nuclear magnetic resonance study of water in food and biological materials. The authors present the methodology, research, and development results of qualitative and quantitative analysis of water in foods and biological materials using NMR and MRI. This book provides the latest NMR and MRI techniques for those researchers who have an interest in relationships between water and: * chemical reactivity * microbial activity * physiochemical properties and changes * structural properties and changes in foods and biological materials In addition, the authors emphasize experimental techniques and data interpretation skills for the study of mobility of water and its role in processing and storage of foods and biological materials. Authors Ruan and Chen explain how the "state of water" concept will greatly add to the reader's understanding of the role of water in chemical, physical and microbial changes occurring in foods and biological materials. Understanding the relationships between water and chemical reactivity, microbial activity, and physiochemical and structural properties and changes in foods is an important key to effective food R&D, as well as quality control in processing and storage. This book provides advanced information on these relationships using the tools of NMR and MRI. Emphasis is placed on experimental techniques and data interpretation skills for the study of mobility of water and its role in processing and storage of foods. Many new techniques and applications are examined. More than 140 schematics, images and graphs illustrate NMR/MRI principles, techniques, applications and results.
Author: Biman Bagchi
Publisher: Cambridge University Press
ISBN: 1107037298
Category : Medical
Languages : en
Pages : 383
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Book Description
A unified overview of the dynamical properties of water and its unique and diverse role in biological and chemical processes.
Author: John L. Finney
Publisher: Oxford University Press, USA
ISBN: 0198708726
Category : Nature
Languages : en
Pages : 161
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Book Description
Around 71% of the Earth's surface is covered in water. In this Very Short Introduction John Finney explores the science of water, its structure and remarkable properties, and its vital role for life on Earth.
Author: Ching T. Hou
Publisher: CRC Press
ISBN: 1420027964
Category : Science
Languages : en
Pages : 614
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Book Description
Until now, no comprehensive handbook on industrial biocatalysis has been available. Soliciting chapters on virtually every aspect of biocatalysis from international experts most actively researching the field, the Handbook of Industrial Biocatalysis fills this need. The handbook is divided into three sections based on types of substrates. T
