The Genetics and Conservation of Spring Run-timing in Chinook Salmon (Oncorhynchus Tshawytscha)

The Genetics and Conservation of Spring Run-timing in Chinook Salmon (Oncorhynchus Tshawytscha) PDF Author: Tasha Thompson
Publisher:
ISBN: 9781658415699
Category :
Languages : en
Pages :

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Book Description
Human-driven environmental changes have altered and reduced phenotypic variation across diverse taxa, but the long-term consequences and the need for conservation action are unclear. This dissertation presents an investigation of the widespread and dramatic decline of the spring-run adult migration phenotype in wild Chinook salmon (Oncorhynchus tshawytscha), caused by dam construction and other anthropogenic activities. First, the Prologue reviews the findings of Prince et al. (2017), which identified the genetic and evolutionary basis of the spring-run phenotype. Next, Chapter 1 explores the mechanism and consequences of the decline and loss of the spring-run phenotype, finding that 1) changes in spring-run phenotype frequency are explained by changes in allele frequency at a single locus, and 2) loss of the spring-run phenotype can be rapidly followed by loss of spring-run alleles (and thus the ability to recover the phenotype). In Chapter 2, a combination of single-locus and genome-wide data is used to explore the utility of a genetic marker for run-type in addressing conservation and management questions in a population of conservation concern (the Chehalis Basin, WA), serving as an example of the ways in which the findings of Chapter 1 can be applied to immediate management questions. Finally, the Epilogue briefly describes the context of this dissertation and its relationship to broader conservation issues.

The Genetics and Conservation of Spring Run-timing in Chinook Salmon (Oncorhynchus Tshawytscha)

The Genetics and Conservation of Spring Run-timing in Chinook Salmon (Oncorhynchus Tshawytscha) PDF Author: Tasha Thompson
Publisher:
ISBN: 9781658415699
Category :
Languages : en
Pages :

Get Book Here

Book Description
Human-driven environmental changes have altered and reduced phenotypic variation across diverse taxa, but the long-term consequences and the need for conservation action are unclear. This dissertation presents an investigation of the widespread and dramatic decline of the spring-run adult migration phenotype in wild Chinook salmon (Oncorhynchus tshawytscha), caused by dam construction and other anthropogenic activities. First, the Prologue reviews the findings of Prince et al. (2017), which identified the genetic and evolutionary basis of the spring-run phenotype. Next, Chapter 1 explores the mechanism and consequences of the decline and loss of the spring-run phenotype, finding that 1) changes in spring-run phenotype frequency are explained by changes in allele frequency at a single locus, and 2) loss of the spring-run phenotype can be rapidly followed by loss of spring-run alleles (and thus the ability to recover the phenotype). In Chapter 2, a combination of single-locus and genome-wide data is used to explore the utility of a genetic marker for run-type in addressing conservation and management questions in a population of conservation concern (the Chehalis Basin, WA), serving as an example of the ways in which the findings of Chapter 1 can be applied to immediate management questions. Finally, the Epilogue briefly describes the context of this dissertation and its relationship to broader conservation issues.

Ecology and Conservation of Spring-run Chinook Salmon

Ecology and Conservation of Spring-run Chinook Salmon PDF Author: Elizabeth A. Campbell
Publisher:
ISBN:
Category : Anadromous fishes
Languages : en
Pages : 80

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Run Timing, Fat Content and Maturity of Spring- and Fall-run Klamath River Chinook Salmon (Oncorhynchus Tshawytscha)

Run Timing, Fat Content and Maturity of Spring- and Fall-run Klamath River Chinook Salmon (Oncorhynchus Tshawytscha) PDF Author: James W. Hearsey
Publisher:
ISBN:
Category : Chinook salmon
Languages : en
Pages : 138

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An Investigation Into the Genetic Basis of Migration Timing in Chinook Salmon (Oncorhynchus Tshawytscha)

An Investigation Into the Genetic Basis of Migration Timing in Chinook Salmon (Oncorhynchus Tshawytscha) PDF Author: Kathleen Gallen O'Malley
Publisher:
ISBN:
Category : Chinook salmon
Languages : en
Pages : 298

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Book Description
The genetic basis of adaptation is complex as many fitness-related traits are quantitative and likely influenced by multiple genes with variable effects across different selective environments. One important adaptation for anadromous Chinook salmon (Oncorhynchus tshawytscha) is the time at which individuals return to natal breeding sites within a reproductive season. Here, I tested for adaptive genetic differentiation among temporally divergent migratory runs using candidate loci. First, I isolated and characterized two copies of the circadian rhythm gene, Clock, from Chinook salmon (OtsClock1a and OtsClock1b). Phylogenetic analysis revealed that these copies likely arose subsequent to the salmonid genome-wide duplication event. Each copy contains a polyglutamine repeat motif (PolyQ), an essential binding domain of this transcription factor. However, a 1200bp non-coding segment, showing >90% sequence identity to the Salmo salar Transferrin gene, is located downstream of the OtsClock1a PolyQ, yet absent from OtsClock1b providing evidence for nonhomologous recombination. Another candidate, Ots515NWFSC, shows sequence identity to three salmonid genes central to reproductive development: Gonadotropin-releasing hormone, CYP19b-I, and Ghrelin. I employed OtsClock1b and Ots515NWFSC to test for differentiation in two unique systems: Feather River, California; and Waitaki and Poulter Rivers, New Zealand. Based on length variation in these candidate genes, Feather River fall and spring run Chinook salmon are genetically differentiated. In contrast, these seasonal runs are genetically indistinguishable based on neutral microsatellite loci. Correspondingly, temporally divergent Chinook salmon runs in the Waitaki and Poulter Rivers show greater differentiation based on variation in OtsClock1b and Ots515NWFSC than microsatellite loci. Tests for selective neutrality indicate that OtsClock1b and Ots515NWFSC are likely under selection in both systems. In addition, I found evidence for a latitudinal cline in two OtsClock1b PolyQ alleles among 42 Chinook salmon runs which is likely maintained by selection and thus may reflect an adaptation to photoperiodic parameters. Lastly, a hierarchical gene diversity analysis of OtsClock1b PolyQ variation revealed that run timing explained 43.7% of the overall genetic variance which contrast sharply with the 8.1% calculated for microsatellite loci. Evidence presented here suggests that OtsClock1b and Ots515NWFSC may influence migration timing of Chinook salmon.

Run Timing, Migratory Patterns, and Harvest Information of Chinook Salmon Stocks Within the Yukon River

Run Timing, Migratory Patterns, and Harvest Information of Chinook Salmon Stocks Within the Yukon River PDF Author:
Publisher:
ISBN:
Category : Chinook salmon
Languages : en
Pages : 44

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A Comparison of Run Timing, Spawn Timing and Spawning Distribution of Natural and Hatchery Spring Chinook Salmon Oncorhynchus Tshawytscha in the Imnaha River, Oregon

A Comparison of Run Timing, Spawn Timing and Spawning Distribution of Natural and Hatchery Spring Chinook Salmon Oncorhynchus Tshawytscha in the Imnaha River, Oregon PDF Author: Timothy L. Hoffnagle
Publisher:
ISBN:
Category : Chinook salmon
Languages : en
Pages : 22

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Status and Future of Spring Chinook Salmon in the Columbia River Basin--conservation and Enhancement

Status and Future of Spring Chinook Salmon in the Columbia River Basin--conservation and Enhancement PDF Author: Donn L. Park
Publisher:
ISBN:
Category : Chinook salmon
Languages : en
Pages : 146

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Salmon in California

Salmon in California PDF Author: Joe Holmberg
Publisher:
ISBN:
Category : Chinook salmon
Languages : en
Pages : 114

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A Conservation Physiology Approach to Understanding the Migration Biology of an Imperiled Stock of Summer-run Chinook Salmon (Oncorhynchus Tshawytscha) in a Regulated River

A Conservation Physiology Approach to Understanding the Migration Biology of an Imperiled Stock of Summer-run Chinook Salmon (Oncorhynchus Tshawytscha) in a Regulated River PDF Author: Caleb Hasler
Publisher:
ISBN:
Category : Aquaculture
Languages : en
Pages : 480

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Neural Gene Expression Profiling in Chinook Salmon (Oncorhynchus Tshawytscha) of Alternate Run Times

Neural Gene Expression Profiling in Chinook Salmon (Oncorhynchus Tshawytscha) of Alternate Run Times PDF Author: Jeremiah C. Bernier
Publisher:
ISBN:
Category : Chinook salmon
Languages : en
Pages : 90

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Book Description
Chinook salmon (Oncorhynchus tshawytscha) display the greatest variability of return times to freshwater of all Pacific salmon. Differential return times to freshwater have segregated populations of Chinook into two broad types or runs, fall and spring, named for the time of year in which they migrate to freshwater. Migration time in Chinook has a strong genetic component, and historically, fall and spring runs were spatially and temporally isolated in watersheds. Using a population of Chinook from California?s Central Valley, I sought to examine gene regulation that results in differential run-timing. Functional genomics has proven to be powerful in distinguishing genes differentially expressed between alternate behaviors in other systems and I used the technique LongSAGE to examine genes differentially regulated between fall and spring run Chinook during freshwater migration. I constructed three LongSAGE libraries from the brains of a spring, fall and ocean sample and observed 221 differentially expressed tags. Using expressed sequence tag (EST) libraries from Atlantic salmon (Salmo salar) and rainbow trout (O. mykiss), I was able to assign putative functions to 59% of differentially expressed tags. Additionally, I tested the expression levels of seven genes in the brains of 8 additional individuals from both runs and found none significantly differentially expressed between fall and spring runs. This study is the first to apply LongSAGE to salmon and provides a framework for conducting future gene expression research into reproduction and migration times, as well as physiology of migrating salmon.