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Author: Ralf Kühn
Publisher: Humana Press
ISBN: 9781934115268
Category : Science
Languages : en
Pages : 0
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Book Description
Following the completion of the mouse and human genome sequences, a major challengeisthefunctionalcharacterizationofeverymammaliangeneandthedeciph- ing of their molecular interaction network. The mouse offers many advantages for the use of genetics to study human biology and disease, unmatched among other m- mals. Its development, body plan, physiology, behavior, and diseases have much in common, based on the fact that 99% of the human genes have a mouse ortholog. The investigation of gene function using mouse models is based on many years of tech- logical development. In the two decades since gene targeting in murine embryonic stem (ES) cells was first described by Mario Capecchi and colleagues, more than 3000 predesigned mouse mutants have been developed. To date, a variety of mouse mutagenesis techniques, either gene- or phenotype-driven, are used as systematic approaches. The availability of the genome sequence supports gene-driven approaches such as gene-trap and targeted mutagenesis in ES cells, allowing efficient and precise gene disruption. In combination with the use of site-specific DNA recombinases, in particular the Cre/loxP system, gene disruptioncan be directed to specific cell types in conditionalmousemutants. Furthermore,chemicalandtransposonmutagenesisofthe mouse genome enables us to perform phenotype-driven screens for the unbiased identification of phenotype–genotype correlations involved in models of human d- ease. Over the next several years, the mouse genome will be systematically altered, and the techniques for achieving predesigned manipulations will be constantly developed further and improved. The second edition of Gene Knockout Protocols brings together distinguished c- tributorswithextensiveexperienceinthegenetargetingandmousegeneticsfields.
Author: Ralf Kühn
Publisher: Humana Press
ISBN: 9781934115268
Category : Science
Languages : en
Pages : 0
Get Book Here
Book Description
Following the completion of the mouse and human genome sequences, a major challengeisthefunctionalcharacterizationofeverymammaliangeneandthedeciph- ing of their molecular interaction network. The mouse offers many advantages for the use of genetics to study human biology and disease, unmatched among other m- mals. Its development, body plan, physiology, behavior, and diseases have much in common, based on the fact that 99% of the human genes have a mouse ortholog. The investigation of gene function using mouse models is based on many years of tech- logical development. In the two decades since gene targeting in murine embryonic stem (ES) cells was first described by Mario Capecchi and colleagues, more than 3000 predesigned mouse mutants have been developed. To date, a variety of mouse mutagenesis techniques, either gene- or phenotype-driven, are used as systematic approaches. The availability of the genome sequence supports gene-driven approaches such as gene-trap and targeted mutagenesis in ES cells, allowing efficient and precise gene disruption. In combination with the use of site-specific DNA recombinases, in particular the Cre/loxP system, gene disruptioncan be directed to specific cell types in conditionalmousemutants. Furthermore,chemicalandtransposonmutagenesisofthe mouse genome enables us to perform phenotype-driven screens for the unbiased identification of phenotype–genotype correlations involved in models of human d- ease. Over the next several years, the mouse genome will be systematically altered, and the techniques for achieving predesigned manipulations will be constantly developed further and improved. The second edition of Gene Knockout Protocols brings together distinguished c- tributorswithextensiveexperienceinthegenetargetingandmousegeneticsfields.
Author: Ke-Qin Zhang
Publisher: Springer Science & Business
ISBN: 9401787301
Category : Science
Languages : en
Pages : 400
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Book Description
These chapters provide up-to-date information on nematophagous fungi, particularly those of the Orbiliaceae in Ascomycota, whose asexual states produce nematode-trapping devices. The authors consider fungal-nematode interactions, fossil fungi, the biodiversity, ecology and geographical distribution of nematode-trapping fungi, and their potential use in biocontrol of nematodes, all in detail. Nematode-trapping fungi with adhesive or mechanical hyphal traps are the main focus of this book which begins with an overview of the data on nematode-trapping fungi, including their taxonomy, phylogeny and evolution. Subsequent chapters expand upon the methods and techniques used to study these fascinating fungi. Keys for genera of Arthrobotrys, Drechslerella and Dactylellina, which include all reported species of predatory orbiliaceous fungi are presented and numerous species from these genera are morphologically described and illustrated. The ecology of nematode-trapping fungi is expertly presented: their occurrence and habitats, their geographical and seasonal distribution and the effects of soil conditions and nematode density on their distribution all feature amongst the relevant themes. Further chapters examine the use of nematode-trapping fungi in biological control and the authors consider nematicidal activities in detail, exploring the many compounds from fungi that feature in nematicidal activities and of course useful paths for further study on this topic. This is a highly informative and carefully presented book, providing scientific insight for scholars with an interest in fungi and in biological control of nematodes.
Author:
Publisher: Academic Press
ISBN: 9780123747754
Category : Science
Languages : en
Pages : 536
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Book Description
Guide to Techniques in Mouse Development, Part A comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. Update of volume 225 of Methods in Enzymology, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993 Covers new technologies and methodologies, including: new techniques for the cryopreservation of gametes and embryos production of transgenic and null (knockout) animals (use of ES cells) generation of conditional/inducible mutant animals use of gene-trap mutagenesis analysis of allele-specific expresion use of new reporter constructs humanizing of transgenic animals transcript profiling of mouse development imaging of mouse development rederivation of animals and use of mouse genomics
Author: Silja Samerski
Publisher: Andrews UK Limited
ISBN: 1845408306
Category : Medical
Languages : en
Pages : 173
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Book Description
The Decision Trap questions a dogma of our time: the assumption that genetic education empowers citizens and increases their autonomy. It argues that professional instructions about genes, genetic risks, and genetic test options convey a genetic worldview which destroys self-confidence and makes clients dependent on genetic experts and technologies. Part one of the book introduces the reader to the idea of genetic education. It clarifies the notion of the "gene" as it is commonly understood, and shows that, scientifically, the concept of genes as definable, causal agents is outdated. Part two of the book investigates the hidden curriculum of genetic education, using genetic counselling as a prime example. Genetic counselling is a professional service that aims to enable clients to make autonomous decisions about genetic test options and cope with the results.
Author: Suzanna Danuta Walters
Publisher: NYU Press
ISBN: 0814770584
Category : Social Science
Languages : en
Pages : 345
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Book Description
Froma Glee ato gay marriage, from lesbian senators to out gay Marines, we have undoubtedly experienced a seismic shift in attitudes about gays in American politics and culture. Our reigning national story is that a new era of rainbow acceptance is at hand. But dig a bit deeper, and this seemingly brave new gay world is disappointing. For all of the undeniable changes, the plea for tolerance has sabotaged the full integration of gays into American life. Same-sex marriage is unrecognized and unpopular in the vast majority of states, hate crimes proliferate, and even in the much vaunted gay friendly world of Hollywood and celebrity culture, precious few stars are openly gay. Ina The Tolerance Trap, Suzanna Walters takes on received wisdom about gay identities and gay rights, arguing that we are not almost there, but on the contrary have settled for a watered-down goal of tolerance and acceptance rather than a robust claim to full civil rights. After all, wea tolerate aunpleasant realities: medicine with strong side effects, a long commute, an annoying relative. Drawing on a vast array of sources and sharing her own personal journey, Walters shows how the low bar of tolerance demeans rather than ennobles both gays and straights alike. Her fascinating examination covers the gains in political inclusion and the persistence of anti-gay laws, the easy-out sexual freedom of queer youth and the suicides and murders of those in decidedly intolerant environments. She challenges both born that way storylines that root civil rights in biology, and god made me that way arguments that similarly situate sexuality as innate and impervious to decisions we make to shape it. A sharp and provocative cultural critique, this book deftly argues that a too-soon declaration of victory short-circuits full equality and deprives us all of the transformative possibilities of full integration.Tolerance is not the end goal, but a dead end. Ina The Tolerance Trap, Walters presents a complicated snapshot of a world-shifting moment in American historyOCoone that is both a wake-up call and a call to arms for anyone seeking true equality."
Author: Hemin R. Chin
Publisher: CRC Press
ISBN: 1420038478
Category : Science
Languages : en
Pages : 360
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Book Description
The past few years have witnessed extraordinary advances in molecular genetic techniques and the accumulation of structural genomics information and resources in both human and model organisms. With the development of new technologies and the availability of resources like the sequence of eukaryotic genomes, problems of a previously unthinkable sco
Author: Robert Feil
Publisher: Springer
ISBN: 9783642071218
Category : Medical
Languages : en
Pages : 0
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Book Description
In this book, leading experts provide timely and comprehensive information on methods for conditional mutagenesis in the mouse and their application to model human physiology and pathophysiology. The book illustrates how sophisticated genetic manipulations of the mouse genome are employed to model human diseases and to identify underlying molecular mechanisms. In addition, it considers the development of new drugs to treat human diseases.
Author: Andrew Fukuda
Publisher: St. Martin's Griffin
ISBN: 125002076X
Category : Young Adult Fiction
Languages : en
Pages : 322
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Book Description
From author Andrew Fukuda comes The Trap, the explosive finale to The Hunt trilogy—perfect for fans of The Hunger Games! After barely escaping the Mission alive, Gene and Sissy face an impossible task: staying alive long enough to stop an entire world bent on their destruction. Bound on a train heading into the unknown with the surviving Mission girls, Gene, Sissy, David, and Epap must stick together and use everything they have to protect each other and their only hope: the cure that will turn the blood-thirsty creatures around them into humans again. Now that they know how to reverse the virus, Gene and Sissy have one final chance to save those they love and create a better life for themselves. But as they struggle to get there, Gene's mission sets him on a crash course with Ashley June, his first love . . . and his deadliest enemy.
Author: Michael P. Starkey
Publisher: Springer Science & Business Media
ISBN: 159259235X
Category : Medical
Languages : en
Pages : 537
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Book Description
We must unashamedly admit that a large part of the motivation for editing Genomics Protocols was selfish. The possibility of assembling in a single volume a unique and comprehensive collection of complete protocols, relevant to our work and the work of our colleagues, was too good an opportunity to miss. We are pleased to report, however, that the outcome is something of use not only to those who are experienced practitioners in the genomics field, but is also valuable to the larger community of researchers who have recognized the potential of genomics research and may themselves be beginning to explore the technologies involved. Some of the techniques described in Genomics Protocols are clearly not restricted to the genomics field; indeed, a prerequisite for many procedures in this discipline is that they require an extremely high throughput, beyond the scope of the average investigator. However, what we have endeavored here to achieve is both to compile a collection of procedures concerned with geno- scale investigations and to incorporate the key components of “bottom-up” and “top-down” approaches to gene finding. The technologies described extend from those traditionally recognized as coming under the genomics umbrella, touch on proteomics (the study of the expressed protein complement of the genome), through to early therapeutic approaches utilizing the potential of genome programs via gene therapy (Chapters 27–30).
Author: Hans Hedrich
Publisher: Academic Press
ISBN: 012382009X
Category : Science
Languages : en
Pages : 869
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Book Description
The Laboratory Mouse, Second Edition is a comprehensive book written by international experts. With inclusions of the newly revised European standards on laboratory animals, this will be the most current, global authority on the care of mice in laboratory research. This well-illustrated edition offers new and updated chapters including immunology, viruses and parasites, behavior, enrichment and care standards of laboratory mice across the life sciences, medical and veterinary fields. - Features four-color illustrations with complete instruction on mouse surgery, anatomy, behavior and care of the mouse in laboratory research - Offers additional chapters on new mouse strains, phenotyping of strains, bacteria and parasites, and immunology - Includes the newly revised EU standards on care, as well as, comparisons to standards and regulations in the US and other countries
