Modern Methods of Steroid Analysis PDF Download
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Author: Erich Heftmann
Publisher: Elsevier
ISBN: 0323146449
Category : Science
Languages : en
Pages : 558
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Book Description
Modern Methods of Steroid Analysis reviews modern methods of steroid analysis such as liquid column chromatography, mass spectrometry, and gas chromatography. Topics covered include qualitative and quantitative analysis of plant sterols by gas-liquid chromatography; Raman spectroscopy of steroids; nuclear magnetic resonance; and applications of lanthanide shift reagents. A computerized method for rapid comparison and retrieval of infrared spectral data is also described. This volume is comprised of 22 chapters and begins with an analysis of hormonal steroids using liquid column chromatography, followed by a discussion on the separation of insect molting hormones using high-pressure liquid chromatography. The reader is methodically introduced to the application of gradient elution and thin-layer chromatography to the analysis of corticosteroids and 17-ketosteroids, as well as some aspects of mass spectrometry in steroid analysis. Subsequent chapters explore other methods such as optical rotatory dispersion and circular dichroism, along with radioisotope techniques. In particular, the radioimmunoassay of plasma steroid hormones and plasma aldosterone is considered. This book should be of interest to biochemists.
Author: Erich Heftmann
Publisher: Elsevier
ISBN: 0323146449
Category : Science
Languages : en
Pages : 558
Get Book Here
Book Description
Modern Methods of Steroid Analysis reviews modern methods of steroid analysis such as liquid column chromatography, mass spectrometry, and gas chromatography. Topics covered include qualitative and quantitative analysis of plant sterols by gas-liquid chromatography; Raman spectroscopy of steroids; nuclear magnetic resonance; and applications of lanthanide shift reagents. A computerized method for rapid comparison and retrieval of infrared spectral data is also described. This volume is comprised of 22 chapters and begins with an analysis of hormonal steroids using liquid column chromatography, followed by a discussion on the separation of insect molting hormones using high-pressure liquid chromatography. The reader is methodically introduced to the application of gradient elution and thin-layer chromatography to the analysis of corticosteroids and 17-ketosteroids, as well as some aspects of mass spectrometry in steroid analysis. Subsequent chapters explore other methods such as optical rotatory dispersion and circular dichroism, along with radioisotope techniques. In particular, the radioimmunoassay of plasma steroid hormones and plasma aldosterone is considered. This book should be of interest to biochemists.
Author: Ian Elcock Bush
Publisher: Pergamon
ISBN:
Category : Medical
Languages : en
Pages : 474
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Book Description
The basic theory of chromatography and some general principles; Chromatographic separation of steroids; Techniques and apparatus; Quantitative chromatography: colorimetric and radioisotopic techniques; Structural analysis and identification of steroids by chromatography; Some typical analytical problems of steroid biochemistry; Purification of reagents and materials; Microchemical reactions for steroids; Methods of detection on paper chromatograms; Calculations with Rf and Rm value.
Author: Kristen B. Eik-Nes
Publisher: Springer
ISBN: 9783642867958
Category : Science
Languages : en
Pages : 0
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Book Description
Few fields have advanced faster over the past quinquennium than separation and estimation of steroids by the technique of gas phase chromatography. A detailed and complete review of this topic would therefore be beyond the scope of the authors contributing to this monograph. It was, however, felt that a discussion of some of the highlights of this rapid advance might be of help for laboratories estimating steroids in biological samples. One of the difficulties in producing a monograph of this kind is that before it can appear in print it is likely that some of the methods it discusses will have been overtaken by better methods, so swiftly is progress now made. No editorial power has been exercised in trying to make a uni form account of technology in this field, and the idiosyncrasies of each individual author have been left intact. Through this approach we hope that what has been lost in scholarly appearance is regained in general appeal.
Author: Joseph Sherma
Publisher: CRC Press
ISBN: 0824748662
Category : Science
Languages : en
Pages : 1356
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Book Description
In this third edition, more than 40 renowned authorities introduce and update chapters on the theory, fundamentals, techniques, and instrumentation of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC), highlighting the latest procedures and applications of TLC to 19 important compound classes and coverage of TLC applications by compound type. Easily adaptable to industrial scenarios , the Handbook of Thin-Layer Chromatography, Third Edition supports practical research strategies with extensive tables of data, offers numerous figures that illustrate techniques and chromatograms, and includes a glossary as well as a directory of equipment suppliers.
Author: Richard J. Block
Publisher: Elsevier
ISBN: 1483276872
Category : Science
Languages : en
Pages : 208
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Book Description
Paper Chromatography: A Laboratory Manual focuses on methods, technologies, and processes, and aims to provide readers with a readily accessible source for the uses and adaptations of paper chromatography. The book first offers information on general methods, including descending, ascending, and ascending-descending chromatography, filter paper ""chromatopile"", ""reversed phase"" paper chromatography, and paper electrophoresis. The text then elaborates on quantitative methods and amino acids, amines, and proteins. Discussions focus on visual comparison, elution, area of spot, total color of spot, maximum color density, identification of amines, separation of proteins, and general directions. The publication examines carbohydrates and aliphatic acids and steroids. Topics include simple sugars, miscellaneous derived sugars, and aliphatic acids. The text also ponders on purines, pyrimidines, and related substances and phenols, aromatic acids, and porphyrins. The text is a valuable reference for readers interested in paper chromatography.
Author: Donald H. Chace
Publisher:
ISBN: 9781562386481
Category : Diagnosis, Laboratory
Languages : en
Pages : 97
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Book Description
Author: Wilfried M.A. Niessen
Publisher: CRC Press
ISBN: 1420014544
Category : Medical
Languages : en
Pages : 629
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Book Description
A constructive evaluation of the most significant developments in liquid chromatography-mass spectrometry (LC-MS) and its uses for quantitative bioanalysis and characterization for a diverse range of disciplines, Liquid Chromatography-Mass Spectrometry, Third Edition offers a well-rounded coverage of the latest technological developments and
Author: Q. Alan Xu
Publisher: Springer Science & Business Media
ISBN: 1461438284
Category : Science
Languages : en
Pages : 476
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Book Description
Clinical pharmacology plays an important role in today’s medicine. Due to the high sensitivity, selectivity, and affordability of a mass spectrometer (MS), the high performance liquid chromatography – mass spectrometry (LC-MS) analytical technique is widely used in the determination of drugs in human biological matrixes for clinical pharmacology. Specifically, LC-MS is used to analyze: anticancer drugs antidementia drugs antidepressant drugs antiepileptic drugs antifundal drug antimicrobial drugs antipsychotic drugs antiretroviral drugs anxiolytic/hypnotic drugs cardiac drugs drugs for addiction immunosuppressant drugs mood stabilizer drugs This book will primarily cover the various methods of validation for LC-MS techniques and applications used in modern clinical pharmacology.
Author: Jack Cazes
Publisher: CRC Press
ISBN: 1439870675
Category : Science
Languages : en
Pages : 2968
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Book Description
Thoroughly revised and expanded, this third edition offers illustrative tables and figures to clarify technical points in the articles and provides a valuable, reader-friendly reference for all those who employ chromatographic methods for analysis of complex mixtures of substances. An authoritative source of information, this introductory guide to specific chromatographic techniques and theory discusses the relevant science and technology, offering key references for analyzing specific chemicals and applications in industry and focusing on emerging technologies and uses.
Author: Lukasz Komsta
Publisher: CRC Press
ISBN: 1466507152
Category : Medical
Languages : en
Pages : 1070
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Book Description
Used routinely in drug control laboratories, forensic laboratories, and as a research tool, thin layer chromatography (TLC) plays an important role in pharmaceutical drug analyses. It requires less complicated or expensive equipment than other techniques, and has the ability to be performed under field conditions. Filling the need for an up-to-date, complete reference, Thin Layer Chromatography in Drug Analysis covers the most important methods in pharmaceutical applications of TLC, namely, analysis of bulk drug material and pharmaceutical formulations, degradation studies, analysis of biological samples, optimization of the separation of drug classes, and lipophilicity estimation. The book is divided into two parts. Part I is devoted to general topics related to TLC in the context of drug analysis, including the chemical basis of TLC, sample pleparation, the optimization of layers and mobile phases, detection and quantification, analysis of ionic compounds, and separation and analysis of chiral substances. The text addresses the newest advances in TLC instrumentation, two-dimensional TLC, quantification by slit scanning densitometry and image analysis, statistical processing of data, and various detection and identification methods. It also describes the use of TLC for solving a key issue in the drug market—the presence of substandard and counterfeit pharmaceutical products. Part II provides an in-depth overview of a wide range of TLC applications for separation and analysis of particular drug groups. Each chapter contains an introduction about the structures and medicinal actions of the described substances and a literature review of their TLC analysis. A useful resource for chromatographers, pharmacists, analytical chemists, students, and R&D, clinical, and forensic laboratories, this book can be utilized as a manual, reference, and teaching source.
