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Author: Roger L. Lundblad
Publisher: CRC Press
ISBN: 9780849326066
Category : Medical
Languages : en
Pages : 300
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Book Description
This exciting laboratory support book encompasses the encyclopedic coverage of the most frequently used methods for the site-specific chemical modification of proteins. In a concise manner, it presents methods for the characterization of modified proteins, including: amino acid analysis protein sequence analysis chemical cleavage of protein chains chromatographic separation of peptides It also discusses various approaches to the determination of solution protein concentration. It includes a complete literature survey of the various reagents, a list of the most commonly used reagents with their physical and chemical properties, and a list of preferred reagent suppliers.
Author: Roger L. Lundblad
Publisher: CRC Press
ISBN: 9780849326066
Category : Medical
Languages : en
Pages : 300
Get Book
Book Description
This exciting laboratory support book encompasses the encyclopedic coverage of the most frequently used methods for the site-specific chemical modification of proteins. In a concise manner, it presents methods for the characterization of modified proteins, including: amino acid analysis protein sequence analysis chemical cleavage of protein chains chromatographic separation of peptides It also discusses various approaches to the determination of solution protein concentration. It includes a complete literature survey of the various reagents, a list of the most commonly used reagents with their physical and chemical properties, and a list of preferred reagent suppliers.
Author: Tanveer Ali Dar
Publisher: Academic Press
ISBN: 9780128119136
Category : Science
Languages : en
Pages : 0
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Book Description
Protein Modificomics: From Modifications to Clinical Perspectives comprehensively deals with all of the most recent aspects of post-translational modification (PTM) of proteins, including discussions on diseases involving PTMs, such as Alzheimer's, Huntington's, X-linked spinal muscular atrophy-2, aneurysmal bone cyst, angelman syndrome and OFC10. The book also discusses the role PTMs play in plant physiology and the production of medicinally important primary and secondary metabolites. The understanding of PTMs in plants helps us enhance the production of these metabolites without greatly altering the genome, providing robust eukaryotic systems for the production and isolation of desired products without considerable downstream and isolation processes.
Author: Roger L. Lundblad
Publisher: CRC Press
ISBN: 1000697797
Category : Medical
Languages : en
Pages : 550
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Book Description
First published in 1991, Chemical Reagents for Protein Modification, 2nd Edition provides a unique combination of theoretical and practical considerations for the use of chemical reagents for site-specific modification of proteins. The book is divided into three sections, with the first section describing general techniques, including information on the organic chemistry of the various modification reactions; the separation and characterization of site-specific modified proteins, including applications to proteins separated by electrophoresis followed by blotting; the specific chemical cleavage of peptide bonds in proteins; the separation of peptides by high-performance liquid chromatography and electrophoresis; and the use of chemical reagents to assess conformational change in proteins. The second section provides an encyclopedic description of reagents and reactions for the site-specific modification of individual amino acid residues in proteins. The final section presents descriptions of the use of chemical reagents to label biologically significant sites in proteins, including enzyme active sites and the use of covalent cross-linking to measure protein-protein interactions. Particular emphasis is placed on the use of photoaffinity reagents. The book will be an extremely useful research tool for all investigators interested in the solution chemistry of proteins.
Author: Roger L. Lundblad
Publisher: Springer Science & Business
ISBN: 9780849319839
Category : Medical
Languages : en
Pages : 360
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Book Description
Revised and updated, Chemical Reagents for Protein Modification, Third Edition is an encyclopedic work describing the many approaches to the site-specific modification of proteins. More than 2,000 references are cited describing the development of the various reagents. This book explores how the use of site-specific protein modification relates to a wide variety of biotechnology processes and products, such as protein microarrays, hydrogels, controlled-release pharmaceuticals, and biotherapeutics, and proteomics. With chapters focusing on analyses of individual amino acids, additional chapters cover chemical cross-linking and the chemical cleavage of peptide chains. There is considerable reference to the use of site-specific chemical modification in proteomics. The author pays particular attention to the general environmental factors that influence the reactivity of individual amino acid residues. He presents techniques for the characterization of modified proteins, including mass spectrometry, and includes increased coverage of spectroscopic techniques and crystallographic analyses. This is followed by coverage of nitrosylation reactions and other non-enzymatic modification processes. Specific reference is given to the differential labeling of protein functional groups with reagents labeled with stable isotopes. Resources include a list of current journals that publish site-specific chemical modification studies, the most commonly used reagents and their properties, and a contact list of reagent suppliers. Chemical Reagents for Protein Modification, 3rd Edition presents the most frequently used methods for the site-specific chemical modification of proteins, techniques for protein characterization, precise laboratory data for factors that influence reactivity and reproducibility, and industry-specific resources.
Author: Roger L. Lundblad
Publisher: CRC Press
ISBN: 1466571918
Category : Medical
Languages : en
Pages : 678
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Book Description
The use of the chemical modification of proteins has evolved over the past 80 years, benefiting from advances in analytical, physical, and organic chemistry. Over the past 30 years, the use of chemical reagents to modify proteins has been crucial in determining the function and structure of purified proteins. This groundbreaking work is part of the
Author: Oscar Alzate
Publisher: CRC Press
ISBN: 1420076264
Category : Medical
Languages : en
Pages : 356
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Book Description
In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg
Author: Roger L. Lundblad
Publisher: Springer
ISBN:
Category : Science
Languages : en
Pages : 200
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Book Description
The chemical modification of proteins; Amino acid analysis; Peptide separation by reverse-phase high performance liquid chromatography; Methods for sequence determination; Chemical cleavage of peptide bonds; The modification of cystine; The Modification of cystine - cleavage of disulfide bonds; The modification of methionine; Modification of histidine residues; The modification of Lysine.
Author: Christoph Kannicht
Publisher: Springer Science & Business Media
ISBN: 1592591817
Category : Science
Languages : en
Pages : 326
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Book Description
Christoph Kannicht and a panel of highly experienced researchers describe readily reproducible methods for detecting and analyzing the posttranslational modifications of protein, particularly with regard to protein function, proteome research, and the characterization of pharmaceutical proteins.
Author: Roger L. Lundblad
Publisher: Springer
ISBN:
Category : Science
Languages : en
Pages : 194
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Book Description
The chemical modification of proteins; Amino acid analysis; Peptide separation by reverse-phase high performance liquid chromatography; Methods for sequence determination; Chemical cleavage of peptide bonds; The modification of cystine; The Modification of cystine - cleavage of disulfide bonds; The modification of methionine; Modification of histidine residues; The modification of Lysine.
Author: Greg T. Hermanson
Publisher: Academic Press
ISBN: 0080568726
Category : Science
Languages : en
Pages : 1233
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Book Description
Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions
