The Use of CRISPR/cas9, ZFNs, TALENs in Generating Site-Specific Genome Alterations PDF Download
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Author:
Publisher: Academic Press
ISBN: 0128013346
Category : Science
Languages : en
Pages : 594
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Book Description
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in biomineralization science Contains sections on such topics as genome editing, genome engineering, CRISPR, Cas9, TALEN and zinc finger nuclease
Author:
Publisher: Academic Press
ISBN: 0128013346
Category : Science
Languages : en
Pages : 594
Get Book Here
Book Description
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in biomineralization science Contains sections on such topics as genome editing, genome engineering, CRISPR, Cas9, TALEN and zinc finger nuclease
Author: Jennifer A. Doudna
Publisher: Academic Press
ISBN: 9780128011850
Category : Medical
Languages : en
Pages : 549
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Book Description
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in biomineralization science Contains sections on such topics as genome editing, genome engineering, CRISPR, Cas9, TALEN and zinc finger nuclease
Author: Takashi Yamamoto
Publisher: Springer
ISBN: 4431552278
Category : Science
Languages : en
Pages : 206
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Book Description
This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields.
Author: United States. Congress. House. Committee on Energy and Commerce. Subcommittee on Commerce, Consumer Protection, and Competitiveness
Publisher:
ISBN:
Category :
Languages : en
Pages : 400
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Book Description
Author: David Eddings
Publisher: Del Rey
ISBN: 0345367693
Category : Fiction
Languages : en
Pages : 450
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Book Description
From the #1 New York Times bestselling author of the Belegariad and Mallorean series comes the first novel in a series imbued with magic and adventure. Sparhawk, Pandion Knight and Queen’s Champion, returned to Elenia after ten years of exile, only to find his young Queen Ehlana trapped in a block of ensorcelled crystal. Only the great sorcery of Sephrenia, ageless instructor of magic, kept her alive—but the spell would last only a year, and its cost was tragically high. Now a Prince Regent ruled Elenia, the puppet of Annias, ambitious Primate of the Church who planned to seize power over all the land. As Sparhawk and Sephrenia set out to find a cure for Ehlana, Sephrenia revealed that there was only one person in the west who could defeat the evil plots against Ehlana. That person was Sparhawk.
Author: Emily Talen
Publisher: Routledge
ISBN: 1135992614
Category : Architecture
Languages : en
Pages : 336
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Book Description
New Urbanism and American Planning presents the history of American planners’ quest for good cities and shows how New Urbanism is a culmination of ideas that have been evolving since the nineteenth century. In her survey of the last hundred or so years of urbanist ideals, Emily Talen identifies four approaches to city-making, which she terms ‘cultures’: incrementalism, plan-making, planned communities, and regionalism. She shows how these cultures connect, overlap, and conflict and how most of the ideas about building better settlements are recurrent. In the first part of the book Talen sets her theoretical framework and in the second part provides detailed analysis of her four ‘cultures’.She concludes with an assessment of the successes and failures of the four cultures and the need to integrate these ideas as a means to promoting good urbanism in America.
Author: Emily Talen
Publisher: Island Press
ISBN: 1610911768
Category : Architecture
Languages : en
Pages : 257
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Book Description
City Rules offers a challenge to students and professionals in urban planning, design, and policy to change the rules of city-building, using regulations to reinvigorate, rather than stifle, our communities. Emily Talen demonstrates that regulations are a primary detriment to the creation of a desirable urban form. While many contemporary codes encourage sprawl and even urban blight, that hasn't always been the case-and it shouldn't be in the future. Talen provides a visually rich history, showing how certain eras used rules to produce beautiful, walkable, and sustainable communities, while others created just the opposite. She makes complex regulations understandable, demystifying city rules like zoning and illustrating how written codes translate into real-world consequences. Most importantly, Talen proposes changes to these rules that will actually enhance communities' freedom to develop unique spaces.
Author: Emily Talen
Publisher: Routledge
ISBN: 9781932364637
Category : City planning
Languages : en
Pages : 0
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Book Description
Designing a good community entails so much more than architecture. But too often architects lead the discussion on urban design. In her clarion call for real community-based urban design, Emily Talen challenges planners to reengage in urban design to ensure that it supports and promotes diverse, sustainable, vibrant, and equitable communities. This book is a step-by-step guide to observing, analyzing, and designing civic-minded, well -functioning, and pedestrian-oriented places. For planners to connect urban design to social objectives, they need the proper urban design tools, and this book provides them. It is organized as an easy-to-use manual of 10 exercises that rely on three types of software: mapping/spatial analysis (GIS), 3-D modeling (SketchUp), and graphics (Adobe Illustrator and Photoshop). The book includes an extensive list of discussion questions and additional activities to help planners understand and execute urban design. Urban Design Reclaimed is for anyone with an interest in the built environment.
Author: Jose Cibelli
Publisher: Academic Press
ISBN: 0123865425
Category : Science
Languages : en
Pages : 585
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Book Description
Principles of Cloning, Second Edition is the fully revised edition of the authoritative book on the science of cloning. The book presents the basic biological mechanisms of how cloning works and progresses to discuss current and potential applications in basic biology, agriculture, biotechnology, and medicine. Beginning with the history and theory behind cloning, the book goes on to examine methods of micromanipulation, nuclear transfer, genetic modification, and pregnancy and neonatal care of cloned animals. The cloning of various species—including mice, sheep, cattle, and non-mammals—is considered as well. The Editors have been involved in a number of breakthroughs using cloning technique, including the first demonstration that cloning works in differentiated cells done by the Recipient of the 2012 Nobel Prize for Physiology or Medicine – Dr John Gurdon; the cloning of the first mammal from a somatic cell – Drs Keith Campbell and Ian Wilmut; the demonstration that cloning can reset the biological clock - Drs Michael West and Robert Lanza; the demonstration that a terminally differentiated cell can give rise to a whole new individual – Dr Rudolf Jaenisch and the cloning of the first transgenic bovine from a differentiated cell – Dr Jose Cibelli. The majority of the contributing authors are the principal investigators on each of the animal species cloned to date and are expertly qualified to present the state-of-the-art information in their respective areas. First and most comprehensive book on animal cloning, 100% revised Describes an in-depth analysis of current limitations of the technology and research areas to explore Offers cloning applications on basic biology, agriculture, biotechnology, and medicine
Author: Essam M. Abdelalim
Publisher: Springer
ISBN: 3319332708
Category : Medical
Languages : en
Pages : 276
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Book Description
This volume explores recent advances in the use of pluripotent stem cells (PSCs) and adult stem cells (ASCs) in basic and clinical applications. The chapters discuss use of PSCs for drug screening, genome editing, modeling of kidney, motor neuron diseases, and diabetes as well as their application in cancer; ASCs are discussed in the contexts of banking of umbilical cord stem cells, use of multipotential stromal cells (MSCs) for bone repair, cellular interactions during fracture repair stages, and therapeutic applications of neural crest stem cells and lung stem cells. The text is organized by sections dealing with PSCs and ASCs specifically, presenting the reader with a comprehensive examination of both these forms of stem cells. Expertly authored and drawing from a wealth of international perspectives, Recent Advances in Stem Cells: From Basic Research to Clinical Applications presents a succinct yet detailed review of cutting-edge research in this rapidly expanding field. This installment of Springer’s Stem Cell Biology and Regenerative Medicine series is essential reading for academics, researchers, and clinicians in the fields of cell biology, genetics, nephrology, osteology, oncology, and pulmonology.
