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Author: Viktor Stein
Publisher:
ISBN: 9781493969401
Category : Biotechnology
Languages : en
Pages : 341
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Book Description
This detailed book provides state-of-the-art protocols to facilitate the construction of synthetic protein switches for a variety of applications in biotechnology and basic research. Beginning with general strategies, the volume continues with coverage of peptide switches, fluorescent and bioluminescent sensors,?-lactamase sensors, proteolytic sensors, optogenetic switches, as well as cellular signaling switches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synthetic Protein Switches: Methods and Protocols serves as a useful reference for life scientists from diverse research fields that range from traditional, discovery-centered disciplines, like cancer research, to newly emerging disciplines, such as synthetic biology.
Author: Viktor Stein
Publisher:
ISBN: 9781493969401
Category : Biotechnology
Languages : en
Pages : 341
Get Book Here
Book Description
This detailed book provides state-of-the-art protocols to facilitate the construction of synthetic protein switches for a variety of applications in biotechnology and basic research. Beginning with general strategies, the volume continues with coverage of peptide switches, fluorescent and bioluminescent sensors,?-lactamase sensors, proteolytic sensors, optogenetic switches, as well as cellular signaling switches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synthetic Protein Switches: Methods and Protocols serves as a useful reference for life scientists from diverse research fields that range from traditional, discovery-centered disciplines, like cancer research, to newly emerging disciplines, such as synthetic biology.
Author: Lena Maria Karlsson
Publisher:
ISBN:
Category :
Languages : en
Pages : 90
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Book Description
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Book Description
Author: Institute of Medicine
Publisher: National Academies Press
ISBN: 0309219396
Category : Science
Languages : en
Pages : 570
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Book Description
Many potential applications of synthetic and systems biology are relevant to the challenges associated with the detection, surveillance, and responses to emerging and re-emerging infectious diseases. On March 14 and 15, 2011, the Institute of Medicine's (IOM's) Forum on Microbial Threats convened a public workshop in Washington, DC, to explore the current state of the science of synthetic biology, including its dependency on systems biology; discussed the different approaches that scientists are taking to engineer, or reengineer, biological systems; and discussed how the tools and approaches of synthetic and systems biology were being applied to mitigate the risks associated with emerging infectious diseases. The Science and Applications of Synthetic and Systems Biology is organized into sections as a topic-by-topic distillation of the presentations and discussions that took place at the workshop. Its purpose is to present information from relevant experience, to delineate a range of pivotal issues and their respective challenges, and to offer differing perspectives on the topic as discussed and described by the workshop participants. This report also includes a collection of individually authored papers and commentary.
Author: Robert E. Rhoads
Publisher: Humana
ISBN: 9781493936236
Category : Medical
Languages : en
Pages : 0
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Book Description
This volume presents detailed laboratory protocols for in vitro synthesis of mRNA with favorable properties, its introduction into cells by a variety of techniques, and the measurement of physiological and clinical consequences such as protein replacement and cancer immunotherapy. Synthetic techniques are described for structural features in mRNA that provide investigational tools such as fluorescence emission, click chemistry, photo-chemical crosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reduced activation of the innate immune response. Protocols are described for clinical applications such as large-scale transfection of dendritic cells, production of GMP-grade mRNA, redirecting T cell specificity, and use of molecular adjuvants for RNA vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resource for both laboratory investigators and clinicians interested in this powerful and rapidly evolving technology.
Author: Helen Rebecca Ostlund Farrants
Publisher:
ISBN:
Category :
Languages : en
Pages : 134
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Book Description
Mots-clés de l'auteur: Synthetic protein switches ; Allosteric modulation ; Protein engineering ; Antibody fragments ; Antibody mimetics ; Dihydrofolate reductase ; LAMAs.
Author: Jamie Davies
Publisher:
ISBN: 019884154X
Category :
Languages : en
Pages : 137
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Book Description
Written primarily for mid-to-upper level undergraduates, this primer will introduce students to topics at the forefront of the subject that are being applied to probe biological problems, or to address the most pressing issues facing society. These topics will include those that form thecornerstone of contemporary research, helping students to make the transition to active researcher.This primer introduces the challenges and opportunities of applying synthetic biological techniques to mammalian cells, tissues, and organisms. It covers the special features that make engineering mammalian systems different from engineering bacteria, fungi, and plants, and provides an overview ofcurrent techniques. A variety of cutting-edge examples illustrate the different purposes of mammalian synthetic biology, including pure biomedical research, drug production, tissue engineering, and regenerative medicine.
Author: Robert Andrew Langan
Publisher:
ISBN:
Category :
Languages : en
Pages : 87
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Book Description
Allosteric regulation of protein function is widespread in biology, but challenging for de novo protein design as it requires explicit design of multiple states with comparable free energies. We explore the possibility of de novo designing switchable protein systems through modulation of competing inter and intra-molecular interactions. We design a static, five-helix "Cage" with a single interface that can interact either intra-molecularly with a terminal "Latch" helix or inter-molecularly with a peptide "Key". Encoded on the Latch are functional motifs for binding, degradation, or nuclear export that function only when the Key displaces the Latch from the Cage. We describe orthogonal Cage-Key systems that function in vitro, in yeast and in mammalian cells with up to 40-fold activation of function by Key. The design of switchable protein function controlled by induced conformational change is a milestone for de novo protein design and opens up new avenues for synthetic biology and cellular engineering.
Author: Maxim Ryadnov
Publisher: Royal Society of Chemistry
ISBN: 1849737843
Category : Science
Languages : en
Pages : 375
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Book Description
Synthetic biology is a new area of biological research that combines science and engineering in order to design and build novel biological functions and systems. The definition of synthetic biology has been generally accepted as the engineering of biology: the synthesis of complex, biologically based (or inspired) systems, which display functions that do not exist in nature. This engineering perspective may be applied at all levels of the hierarchy of biological structures from individual molecules to whole cells, tissues and organisms. As with any multi-disciplinary field, there is an immense and rapidly-growing body of literature concerning synthetic biology, with several dedicated journals now available. However, locating the best information, or identifying the hottest topics can be time-consuming. This Specialist Periodical Report presents critical and comprehensive reviews of the recent literature in themed chapters prepared by invited authors from across the globe. The series editors are active in the field, ensuring that the most valuable information is presented in an authoritative manner.
Author: Ben L. Feringa
Publisher: John Wiley & Sons
ISBN: 352763441X
Category : Science
Languages : en
Pages : 814
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Book Description
Täglich benutzen wir Schalter, um strombetriebene Geräte an- und abzuschalten und kein Compuer würde ohne sie funktionieren. Nach den gleichen Prinzipien funktionieren auch molekulare Schalter, die unter dem Einfluß ihrer Umwelt zwischen zwei definierten Zuständen wechseln können. Im Gegensatz zu den gewöhnlichen Schaltern sind molekulare Schalter aber außerordentlich klein und ihre Anwendung in der Nanotechnologie, Biomedizin und im Computerchipdesign öffnet neue Horizonte. Im vorliegenden Zweibänder berichten Herausgeber und Autoren über molekulare Schalter aus Katenanen und Rotaxanen, Fulgiden, Flüssigkristallen und Polypeptiden. Die Bandbreite der behandelten Themen reicht von chiroptischen Schaltern über multifunktionale Systeme bis hin zu molekularen logischen Schaltungen. Chemiker und Materialwissenschaftler in Industrie und Hochschule, die sich für einen der innovativsten Bereiche ihrer Wissenschaft interessieren, werden dieses Buch mit Gewinn lesen!
