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Author: Yash Jain
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Our previous research has shown that natural killer (NK) cells play a vital role as immune effectors in the selection and differentiation of various cancer stem cells and undifferentiated tumors. They achieve this through the direct killing and the release of IFN-[gamma] and TNF-[alpha], which inhibit tumor growth and curtail metastasis. Mechanistically, our studies have revealed that NK cells expanded by Osteoclasts display the ability to lyse CD4+ T cells while sparing CD8+ T cells, suggesting a potential selective effect of OC-activated NK cells on CD8+ T cell expansion. Consistent with these findings, we observed increased IFN-[gamma] secretion, NK cell-mediated cytotoxicity, and higher proportions of CD8+ T cells in various tissue compartments of oral tumor-bearing hu-BLT mice following immunotherapy with OC-expanded NK cells. In this study, we focused on investigating the lytic and differentiation capabilities of supercharged NK (sNK) cells in ovarian cancer cell lines (OVCAR 4 and OVCAR 8), oral cancer cell lines (OSCC and OSCSC), and a pancreatic cancer cell line (MP 2). Our results demonstrated that sNK cells exhibited greater lysing activity against both poorly differentiated and well differentiated tumors compared to pNK cells treated with IL-2. Furthermore, the supernatants of sNK cells exhibited significantly higher levels of IFN-[gamma] and induced differentiation by an increase in MHC class1 expressions in all the cell lines tested, except for OVCAR 8. Lastly, we observed a downmodulation of NKG2A and CD94 inhibitory receptors on sNK cells, suggesting potential alterations in their functional properties.
Author: Yash Jain
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Our previous research has shown that natural killer (NK) cells play a vital role as immune effectors in the selection and differentiation of various cancer stem cells and undifferentiated tumors. They achieve this through the direct killing and the release of IFN-[gamma] and TNF-[alpha], which inhibit tumor growth and curtail metastasis. Mechanistically, our studies have revealed that NK cells expanded by Osteoclasts display the ability to lyse CD4+ T cells while sparing CD8+ T cells, suggesting a potential selective effect of OC-activated NK cells on CD8+ T cell expansion. Consistent with these findings, we observed increased IFN-[gamma] secretion, NK cell-mediated cytotoxicity, and higher proportions of CD8+ T cells in various tissue compartments of oral tumor-bearing hu-BLT mice following immunotherapy with OC-expanded NK cells. In this study, we focused on investigating the lytic and differentiation capabilities of supercharged NK (sNK) cells in ovarian cancer cell lines (OVCAR 4 and OVCAR 8), oral cancer cell lines (OSCC and OSCSC), and a pancreatic cancer cell line (MP 2). Our results demonstrated that sNK cells exhibited greater lysing activity against both poorly differentiated and well differentiated tumors compared to pNK cells treated with IL-2. Furthermore, the supernatants of sNK cells exhibited significantly higher levels of IFN-[gamma] and induced differentiation by an increase in MHC class1 expressions in all the cell lines tested, except for OVCAR 8. Lastly, we observed a downmodulation of NKG2A and CD94 inhibitory receptors on sNK cells, suggesting potential alterations in their functional properties.
Author: Anahid Jewett
Publisher: Academic Press
ISBN: 0128226269
Category : Science
Languages : en
Pages : 496
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Book Description
NK Cells in Cancer Immunotherapy: Successes and Challenges explains the latest immunotherapeutic strategies, focusing on NK cells to allow the best and precise combination treatments to cancer patients. The book provides existing background knowledge in the field of immunotherapy and discusses future areas of research required to carry out cutting-edge, validated therapies. Chapters cover advances in immunotherapeutic strategies, in particular, the use of NK cells with and without T-cell therapy in the treatment of cancer. The book is a valuable resource for cancer researchers, oncologists, graduate students and those interested in learning more about novel strategies to treat cancer patients. Immunotherapy is fast becoming the method of choice for cancer therapy. Although remarkable advances have been made in the field of immunotherapy, there are significant challenges and difficulties ahead since many of the current immunotherapeutic strategies do not provide long-lasting treatment strategies, and therefore are not very effective. Covers CAR/T and CAR/NK and adoptive NK cell therapy with and without T cell therapies Discusses basic biology of NK cells and mouse models of human cancers and the role of NK cells in metastatic cancer and in cancer stem cells Encompasses information on combination therapies using check point inhibition, adoptive transfer of cytotoxic effector cells, chemotherapeutic drugs and activating and inhibitory antibodies
Author: Benjamin Bonavida
Publisher: Academic Press
ISBN: 0128243961
Category : Science
Languages : en
Pages : 540
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Book Description
Successes and Challenges of NK Immunotherapy: Increasing Anti-tumor Efficacy describes the unique therapeutic applications of NK cells to fight cancers and eliminate the bulk and subset of cancer stem cells responsible for metastasis, relapse and recurrences. The book provides information on the development, engineering, mechanisms of action, response to various preclinical models, and applications in various clinical trials. Sections cover the development of highly engineered cytotoxic NK cells, their mechanisms of action, preclinical and clinical applications, the development and application of CAR-NK cells, and new NK-drug conjugates, also emphasizing that activated NK cells can target and kill highly resistant cancer stem cells. Written by the leading experts on NK immunotherapy worldwide, this is a valuable resource for researchers, clinicians and members of the biomedical field who are interested in understanding novel and efficient therapies to fight cancers. Discusses the unique developmental applications of NK immunotherapy against cancers, which differs greatly from other types of immunotherapies Provides up-to-date and highly relevant information through chapters written by the leading researchers in the field Presents a significant number of schematic diagrams for easy understanding and reproducibility
Author: Khawaja Husnain Haider
Publisher: Walter de Gruyter GmbH & Co KG
ISBN: 3110586916
Category : Medical
Languages : en
Pages : 340
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Book Description
This book is a compilation of the bench experience of leading experts from various research labs involved in the cutting edge area of research. The authors describe the use of stem cells both as part of the combinatorial therapeutic intervention approach and as tools (disease model) during drug development, highlighting the shift from a conventional symptomatic treatment strategy to addressing the root cause of the disease process. The book is a continuum of the previously published book entitled "Stem Cells: from Drug to Drug Discovery" which was published in 2017.
Author: Natasha S. Barteneva
Publisher: Humana Press
ISBN: 9781493976799
Category : Science
Languages : en
Pages : 363
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Book Description
This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts. The chapters provide a comprehensive overview of cellular heterogeneity analysis techniques, exploring cytometry, imaging and spectroscopy methods, metabolic and molecular biological methods in tracking, as well as chromatin and cell cycle heterogeneity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Cellular Heterogeneity: Methods and Protocols encourages readers to explore new ways of studying cells that will help lead to exciting new discoveries.
Author: Neil McKinney
Publisher:
ISBN: 9781894953986
Category : Health & Fitness
Languages : en
Pages : 568
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Book Description
Updated for 2012, this book, Dr. Neil McKinney's fourth on naturopathic oncology, is updated with the rewards of clinical practice, study, research and reader feedback over the last several years. Patients and integrative physicians will find it easier to navigate, more complete, and of real service. DO: use this book to be informed about your best options, and what to expect them to accomplish. THEN: get expert guidance from a licensed, accountable, health professional team experienced in treating cancer. Cancer is a life-threatening disease in most cases. You do not have the objectivity, experience or knowledge to make critical medical decisions alone. This is not just a legal disclaimer! Cancer is unforgiving of delays and poor choices.
Author: Robert A. Meyers
Publisher: Wiley-Blackwell
ISBN: 9783527337897
Category : Science
Languages : de
Pages : 0
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Book Description
The largest high-level encyclopedia on molecular medicine is now publishing a topical volume on Nanomedicine. The long awaited volume gives a comprehensive overview on nanomaterials in drug delivery, imaging and as therapeutics.
Author: Takehiro Izumo
Publisher: Springer
ISBN: 9812879161
Category : Medical
Languages : en
Pages : 251
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Book Description
This book provides a detailed overview of the latest innovations in respiratory endoscopy, from both diagnostic and therapeutic perspectives; each chapter focuses on one disease and the techniques for early diagnosis as well as treatment. It comprehensively covers treatment and procedures, including simultaneous X-ray fluoroscopy and its use during bronchoscopic procedures. This fast-developing technology is essential for the medical management of non-malignant and malignant diseases of the chest, especially lung cancer. Respiratory Endoscopy describes the cooperation between all the members of the healthcare team, and as such is a valuable resource not only for medical staff, but also for radiological technicians and nursing staff who contribute significantly in the care of the patients undergoing these invasive procedures. By promoting teamwork and providing practical know-how, it will improve the success and safety of respiratory endoscopy procedures.
Author: Edwin Choy
Publisher: MDPI
ISBN: 3906980464
Category : Connective tissues
Languages : en
Pages : 157
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Book Description
This book is a printed edition of the Special Issue "Soft Tissue and Bone Sarcoma" that was published in Cancers
Author: Robert Stern
Publisher: Academic Press
ISBN: 0080921086
Category : Medical
Languages : en
Pages : 467
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Book Description
Hyaluronan biology is being recognized as an important regulator of cancer progression. Paradoxically, both hyaluronan (HA) and hyaluronidases, the enzymes that eliminate HA, have also been correlated with cancer progression. Hyaluronan, a long-chain polymer of the extracellular matrix, opens up tissue spaces through which cancer cells move and metastasize. It also confers motility upon cells through interactions of cell-surface HA with the cytoskeleton. Embryonic cells in the process of movement and proliferation use the same strategy. It is an example of how cancer cells have commandeered normal cellular processes for their own survival and spread. There are also parallels between cancer and wound healing, cancer occasionally being defined as a wound that does not heal. The growing body of literature regarding this topic has recently progressed from describing the association of hyaluronan and hyaluronidase expression associated with different cancers, to understanding the mechanisms that drive tumor cell activation, proliferation, drug resistance, etc. No one source, however, discusses hyaluronan synthesis and catabolism, as well as the factors that regulate the balance. This book will offer a comprehensive summary and cutting-edge insight into Hyaluronan biology, the role of the HA receptors, the hyaluronidase enzymes that degrade HA, as well as HA synthesis enzymes and their relationship to cancer. Offers a comprehensive summary and cutting-edge insight into Hyaluronan biology, the role of the HA receptors, the hyaluronidase enzymes that degrade HA, as well as HA synthesis enzymes and their relationship to cancer Chapters are written by the leading international authorities on this subject, from laboratories that focus on the investigation of hyaluronan in cancer initiation, progression, and dissemination Focuses on understanding the mechanisms that drive tumor cell activation, proliferation, and drug resistance
