Spectroscopic Membrane Probes

Spectroscopic Membrane Probes PDF Author: Leslie M. Loew
Publisher: CRC Press
ISBN: 1351093703
Category : Science
Languages : en
Pages : 261

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Book Description
The general picture we have of membranes and of the properties of the proteins imbedded in them, has, arguably emerged directly from probe studies over the past 20 years. This work is designed to make these techniques accessible to a board audience of cell biologists. The techniques discussed revolve primarily around the fluorescence of membrane probes, but applications of light absorption and Raman scattering are included. In addition to reviews of the major applications, most chapters include information on the required apparatus, experimental design, data analysis, and potential pit falls.

Spectroscopic Membrane Probes

Spectroscopic Membrane Probes PDF Author: Leslie M. Loew
Publisher: CRC Press
ISBN: 1351093703
Category : Science
Languages : en
Pages : 261

Get Book Here

Book Description
The general picture we have of membranes and of the properties of the proteins imbedded in them, has, arguably emerged directly from probe studies over the past 20 years. This work is designed to make these techniques accessible to a board audience of cell biologists. The techniques discussed revolve primarily around the fluorescence of membrane probes, but applications of light absorption and Raman scattering are included. In addition to reviews of the major applications, most chapters include information on the required apparatus, experimental design, data analysis, and potential pit falls.

SPECTROSCOPIC MEMBRANE PROBES

SPECTROSCOPIC MEMBRANE PROBES PDF Author: Leslie M. Loew
Publisher: CRC-Press
ISBN: 9780849345371
Category : Science
Languages : en
Pages : 250

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Book Description


Spectroscopic Membrane Probes

Spectroscopic Membrane Probes PDF Author: Leslie M. Loew
Publisher: CRC Press
ISBN: 135109369X
Category : Medical
Languages : en
Pages : 218

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Book Description
The optical spectra of molecular membrane probes can be interpreted terms of the structure, dynamics, and the physiological state of the membrane. The general picture we have of membranes and of the properties of the proteins imbedded in them, has, arguably, emerged directly from probe studies over the last 20 years. This work is designed to make these techniques accessible to a broad audience of cell biologist. The techniques discussed revolve primarily around the fluorescence of membrane probes, but applications of light absorption and Raman scattering are included. In addition to reviews of the major applications, most chapters include information on required apparatus, experimental design, data analysis, and potential pitfalls.

SPECTROSCOPIC MEMBRANE PROBE

SPECTROSCOPIC MEMBRANE PROBE PDF Author: Leslie M. Loew
Publisher: CRC-Press
ISBN: 9780849345364
Category : Science
Languages : en
Pages :

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Book Description


Fluorescent Methods to Study Biological Membranes

Fluorescent Methods to Study Biological Membranes PDF Author: Yves Mely
Publisher: Springer Science & Business Media
ISBN: 3642331270
Category : Science
Languages : en
Pages : 484

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Book Description
Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike.

Fluorescence Spectroscopy, Imaging and Probes

Fluorescence Spectroscopy, Imaging and Probes PDF Author: Ruud Kraayenhof
Publisher: Springer Science & Business Media
ISBN: 3642560679
Category : Science
Languages : en
Pages : 401

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Book Description
The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. This topic book, the second in his series, reflects this exciting scientific progress and deals, among others, with new approaches and new probes in fluorescence spectroscopy, single molecule fluorescence, applications in biomembrane and enzyme studies and imaging of living cells.

Fluorescent Probes

Fluorescent Probes PDF Author: Godfrey S. Beddard
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 256

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Book Description
The photophysical and photochemical properties of probes in biological and in model systems are topics of intense current research. The wavelength, intensity, time and polarisation dependence of fluorescence probes can provide information on properties as diverse as electric fields and changes in conformation and may be used in the formidable task of establishing structure-function relationships in proteins and cell membranes. The wide scope of interactions which can be studied by these fluorescent molecules is illustrated in the articles in this volume which are based on talks, although much extended and updated, given at a meeting on 'Fluorescence Probes in Proteins and Membranes' held in the Royal Institution in November 1979.

Fluorescent and Luminescent Probes for Biological Activity

Fluorescent and Luminescent Probes for Biological Activity PDF Author: W. T. Mason
Publisher: Elsevier
ISBN: 0080531776
Category : Science
Languages : en
Pages : 697

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Book Description
The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes.The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research.TOPICS COVERED:* Single and multiphoton confocal microscopy* Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies* Applications of new optical probes for imaging proteins in gels * Probes and detection technologies for imaging membrane potential in live cells* Use of optical probes to detect microorganisms* Raman and confocal raman microspectroscopy* Fluorescence lifetime imaging microscopy* Digital CCD cameras and their application in biological microscopy

Nanosecond Fluorescence Spectroscopy of Membrane Probes

Nanosecond Fluorescence Spectroscopy of Membrane Probes PDF Author: Edmund Dennis Matayoshi
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Book Description


Lipid Domains

Lipid Domains PDF Author:
Publisher: Academic Press
ISBN: 0128033274
Category : Science
Languages : en
Pages : 393

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Book Description
Current Topics in Membranes is targeted toward scientists and researchers in biochemistry and molecular and cellular biology, providing the necessary membrane research to assist them in discovering the current state of a particular field and in learning where that field is heading. This volume offers an up to date presentation of current knowledge in the field of Lipid Domains. - Written by leading experts - Contains original material, both textual and illustrative, that should become a very relevant reference material - The material is presented in a very comprehensive manner - Both researchers in the field and general readers should find relevant and up-to-date information