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Author: Mouldy Sioud
Publisher: Humana Press
ISBN: 9781603275460
Category : Science
Languages : en
Pages : 472
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Book Description
RNA interference has become a key method in the suppression of gene expression and the development of therapeutic agents, yet there is still the problem of delivery, stability, and the danger of off-target effects such as the silencing of unwanted genes and activation of innate immunity. In siRNA and miRNA Gene Silencing: From Bench to Bedside, expert researchers explore the most recent advances in siRNA design, expression, delivery, in vivo imaging, and methods to minimize siRNA’s unwanted effects and promote successful use in patients. As part of the highly successful Methods in Molecular BiologyTM series, the chapters focus on their respective subjects with easy-to-use, up-to-date information, including several step-by-step laboratory protocols on topics such as new delivery formulations and strategies with promising applications in vitro and in vivo, validated therapeutic target genes, and components of miRNA function, biogenesis, and interference with virus infection. Comprehensive and cutting-edge, siRNA and miRNA Gene Silencing: From Bench to Bedside offers an excellent collection of chapters to aid all those with an interest in RNAi, gene regulation, and new therapies.
Author: Mouldy Sioud
Publisher: Humana Press
ISBN: 9781603275460
Category : Science
Languages : en
Pages : 472
Get Book Here
Book Description
RNA interference has become a key method in the suppression of gene expression and the development of therapeutic agents, yet there is still the problem of delivery, stability, and the danger of off-target effects such as the silencing of unwanted genes and activation of innate immunity. In siRNA and miRNA Gene Silencing: From Bench to Bedside, expert researchers explore the most recent advances in siRNA design, expression, delivery, in vivo imaging, and methods to minimize siRNA’s unwanted effects and promote successful use in patients. As part of the highly successful Methods in Molecular BiologyTM series, the chapters focus on their respective subjects with easy-to-use, up-to-date information, including several step-by-step laboratory protocols on topics such as new delivery formulations and strategies with promising applications in vitro and in vivo, validated therapeutic target genes, and components of miRNA function, biogenesis, and interference with virus infection. Comprehensive and cutting-edge, siRNA and miRNA Gene Silencing: From Bench to Bedside offers an excellent collection of chapters to aid all those with an interest in RNAi, gene regulation, and new therapies.
Author: R. K. Gaur
Publisher: CRC Press
ISBN: 1439873259
Category : Science
Languages : en
Pages : 380
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Book Description
RNAi technology is used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. Introducing new technology in the study of RNA
Author: Gordon Carmichael
Publisher: Springer Science & Business Media
ISBN: 1592599354
Category : Science
Languages : en
Pages : 343
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Book Description
A collection of readily reproducible methods for the design, preparation, and use of RNAs for silencing gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Author: Zhiguo Wang
Publisher: Springer Science & Business Media
ISBN: 364200489X
Category : Science
Languages : en
Pages : 198
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Book Description
MicroRNAs (miRNAs), endogenous noncoding regulatory mRNAs of around 22-nucleotides long, have rapidly emerged as one of the key governors of the gene expression regulatory program in cells of varying species, with ever-increasing implications in the control of the fundamental biological processes and in the pathogenesis of adult humans. The exciting findings in this field have inspired us with a premise and a promise that miRNAs will ultimately be taken to the heart for therapy of human disease. While miRNAs have been considered potential therapeutic targets for disease treatment, it remains obscured what strategies we can use to achieve the goal. In the past years, we have witnessed a rapid evolving of many creative, innovative, inventive strategies and methodologies pertinent to miRNA research and applications. These technologies have convincingly demonstrated their efficacy and reliability in producing gain-of-function or loss-of-function of miRNAs through targeting miRNA expression/biogenesis/function, providing new tools for elucidating miRNA functions and opening up a new avenue for the development of new agents targeting miRNAs for therapeutic aims. The present book provides comprehensive descriptions of these technologies and their applications to miRNA research and to new drug design for miRNA-related diseases. It starts with an overview of up-to-date knowledge of miRNA biology and the potential of miRNAs as therapeutic targets for human disease, followed by an introduction of the new concept of miRNA interference (miRNAi) and the perspectives of miRNAi technologies in general terms. In the following, each chapter introduces one of the miRNAi technologies with detailed descriptions of state-of-the-art design, procedures, principles and applications to basic research, R and D and clinical therapy.
Author: Renato Paro
Publisher: Springer Nature
ISBN: 3030686701
Category : Science
Languages : en
Pages : 215
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Book Description
This open access textbook leads the reader from basic concepts of chromatin structure and function and RNA mechanisms to the understanding of epigenetics, imprinting, regeneration and reprogramming. The textbook treats epigenetic phenomena in animals, as well as plants. Written by four internationally known experts and senior lecturers in this field, it provides a valuable tool for Master- and PhD- students who need to comprehend the principles of epigenetics, or wish to gain a deeper knowledge in this field. After reading this book, the student will: Have an understanding of the basic toolbox of epigenetic regulation Know how genetic and epigenetic information layers are interconnected Be able to explain complex epigenetic phenomena by understanding the structures and principles of the underlying molecular mechanisms Understand how misregulated epigenetic mechanisms can lead to disease
Author: Shao-Yao Ying
Publisher: Springer Science & Business Media
ISBN: 1402085338
Category : Medical
Languages : en
Pages : 461
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Book Description
Nearly 97% of the human genome is the non-coding DNA, which varies from one species to another, and changes in these sequences are frequently noticed to manifest clinical and circumstantial malfunction. Numerous non-protein-coding genes are recently found to encode microRNAs, which are responsible for RNA-mediated gene silencing through RNA interference (RNAi)-like pathways. MicroRNAs (miRNAs), small single-stranded 17–25 nucleotide RNAs capable of interfering with intracellular messenger RNAs (mRNAs) that contain either complete or partial complementarity, are useful for the design of new therapies against cancer polymorphism and viral mutation. Currently over 1000 native miRNA species found in vertebrates and many more new miRNA homologs continue to be identified; however, most of their functions remain to be determined. In this book, many new perspectives of the miRNA research are reviewed and discussed, including their roles in stem cell maintenance, embryonic development, tissue differentiation, adult physiology, disease pathology, cancer research, viral infection, genetic engineering in plants, and utility in cosmetic applications. These new findings may not only provide significant insight into the various mechanisms of miRNAs but also offer a great opportunity in developing new therapeutic interventions.
Author: Praveen Guleria
Publisher: Academic Press
ISBN: 012817336X
Category : Science
Languages : en
Pages : 638
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Book Description
Plant Small RNA: Biogenesis, Regulation and Application describes the biosynthesis of small RNA in plant systems. With an emphasis on the various molecular mechanisms affected by small RNA and their applications in supporting plant growth and survival, this books presents the basics and most recent advancements in small RNA mediated plant genomics, metabolomics, proteomics and physiology. In addition, it emphasizes the various molecular mechanisms affected by small RNA and their applications in supporting plant growth and survival. Final sections cover the most recent advancements in small RNA mediated plant genomics, metabolomics, proteomics and physiology. Presents foundational information about small RNA biology and regulation in plants Includes small RNA pathway advances Describes the application and scope of small RNA technology for agricultural stability
Author: Stefan de Folter
Publisher: Humana Press
ISBN: 9781493990412
Category : Science
Languages : en
Pages : 363
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Book Description
This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book continues by guiding readers through methods on the identification and detection of plant miRNAs, bioinformatic analyses, and strategies for functional analyses of miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant MicroRNAs: Method and Protocols aims to ensure successful results in the further study of this vital area of plant science.
Author: Detlef Weigel
Publisher: CSHL Press
ISBN: 9780879695736
Category : Nature
Languages : en
Pages : 370
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Book Description
The thale cress Arabidopsis thaliana is increasingly popular among plant scientists: it is small, easy to grow, and makes flowers, and the sequence of its small and simple genome was recently completed. This is the most complete and authoritative laboratory manual to be published on this model organism and the first to deal with genomic and proteomic approaches to its biology.
Author: Dominic W.. Malcolm
Publisher:
ISBN:
Category :
Languages : en
Pages : 202
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Book Description
"Small interfering RNA (siRNA) and microRNA (miRNA) are short double stranded RNA molecules that mediate posttranscriptional gene silencing via RNA interference by binding to or degrading complimentary target mRNA in the cytosol to inhibit translation. Though > 30 siRNA and miRNA candidates are in clinical trials for a variety of indications, none have yet to receive FDA-approval. Successful delivery is the critical barrier to therapeutic translation, and necessitates the need for multifunctional drug delivery systems (DDS). Barriers to delivery include nuclease-mediated degradation and phospholipid membrane impermeability. Furthermore, any DDS used must be cytocompatible and avoid off-target effects. The study herein provides a comprehensive characterization of a pH-responsive diblock copolymer nanoparticle (NP) siRNA DDS and its effects on mesenchymal stem cells (MSCs) for applications in regenerative medicine. Reversible addition-fragmentation chain transfer (RAFT) polymerization was used to synthesize a library of diblock copolymers to determine how polymer properties affect siRNA delivery. Results show this NP-siRNA delivery is effective in multiple therapeutic cell types in vitro, and that increasing NP core hydrophobicity provides greater cytocompatibility and gene silencing. This is achieved by increased pHresponsive membrane lytic activity allowing for better endosomal escape. Additionally, in vitro treatment conditions were identified that resulted in potent gene silencing with no acute cytotoxicity. However, NP-siRNA treatment resulted in a sustained reduction in hMSC metabolic activity, and RNAseq with enrichment analysis shows upregulation of anti-apoptotic gene expression and immune signaling pathways associated with siRNA immune stimulation. Colloidal stability is critical for effective NP DDS, and can be significantly altered in the presence of biological proteins. Therefore, an extensive characterization of this NP-siRNA DDS in the presence of serum reveals NP-siRNA aggregation, which significantly diminishes siRNA delivery in vitro. When delivered locally in vivo, cells take up NP-siRNA complexes in a non-aggregated state, emphasizing the importance of local delivery. Taken together this in-depth characterization of NP-mediated siRNA delivery establishes a solid foundation for the design of next generation NP-siRNA DDS for the delivery of siRNA/miRNA to MSCs."--Pages xiv-xv.
