Micro- and Nanofluidic Devices for Complexed DNA Analysis at the Single Molecule Level PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Micro- and Nanofluidic Devices for Complexed DNA Analysis at the Single Molecule Level PDF full book. Access full book title Micro- and Nanofluidic Devices for Complexed DNA Analysis at the Single Molecule Level by 史利南. Download full books in PDF and EPUB format.
Author: 史利南
Publisher:
ISBN:
Category :
Languages : en
Pages :
Get Book Here
Book Description
Author: 史利南
Publisher:
ISBN:
Category :
Languages : en
Pages :
Get Book Here
Book Description
Author: Miao Yu
Publisher:
ISBN:
Category : DNA
Languages : en
Pages : 130
Get Book Here
Book Description
Author: James Goh
Publisher: Springer Science & Business Media
ISBN: 3319029134
Category : Technology & Engineering
Languages : en
Pages : 998
Get Book Here
Book Description
This volume presents the processing of the 15th ICMBE held from 4th to 7th December 2013, Singapore. Biomedical engineering is applied in most aspects of our healthcare ecosystem. From electronic health records to diagnostic tools to therapeutic, rehabilitative and regenerative treatments, the work of biomedical engineers is evident. Biomedical engineers work at the intersection of engineering, life sciences and healthcare. The engineers would use principles from applied science including mechanical, electrical, chemical and computer engineering together with physical sciences including physics, chemistry and mathematics to apply them to biology and medicine. Applying such concepts to the human body is very much the same concepts that go into building and programming a machine. The goal is to better understand, replace or fix a target system to ultimately improve the quality of healthcare. With this understanding, the conference proceedings offer a single platform for individuals and organizations working in the biomedical engineering related field to gather and network with each other in so doing create the catalyst for future development of biomedical engineering in Asia.
Author: Brian J. Kirby
Publisher: Cambridge University Press
ISBN: 1139489836
Category : Technology & Engineering
Languages : en
Pages : 536
Get Book Here
Book Description
This text focuses on the physics of fluid transport in micro- and nanofabricated liquid-phase systems, with consideration of gas bubbles, solid particles, and macromolecules. This text was designed with the goal of bringing together several areas that are often taught separately - namely, fluid mechanics, electrodynamics, and interfacial chemistry and electrochemistry - with a focused goal of preparing the modern microfluidics researcher to analyse and model continuum fluid mechanical systems encountered when working with micro- and nanofabricated devices. This text serves as a useful reference for practising researchers but is designed primarily for classroom instruction. Worked sample problems are included throughout to assist the student, and exercises at the end of each chapter help facilitate class learning.
Author: Kazuma Mawatari
Publisher: World Scientific
ISBN: 1848168012
Category : Science
Languages : en
Pages : 187
Get Book Here
Book Description
For the past decade, new research fields utilizing microfluidics have been formed. General micro-integration methods were proposed, and the supporting fundamental technologies were widely developed. These methodologies have made various applications in the fields of analytical and chemical synthesis, and their superior performances such as rapid, simple, and high efficient processing have been proved. Recently, the space is further downscaling to 101 103nm scale (we call the space extended-nano space). The extended-nano space located between the conventional nanotechnology (100 101nm) and microtechnology (>1 m), and the research tools are not well established. In addition, the extended-nano space is a transient space from single molecules to bulk condensed phase, and fluidics and chemistry are not unknown. For these purposes, basic methodologies were developed, and new specific phenomena in fluidics and chemistry were found. These new phenomena were applied to unique chemical operations such as concentration and ion selection. The new research fields which are now being created are quite different from those in microspace. Unique devices are also increasingly being reported. In this book, we describe the fundamental technologies for extended-nano space and show the unique liquid properties found in this space and applications for single molecule or cell analysis. The research area is very new and hence, exciting. In contrast to other specialized areas, the research fields require wide knowledge (chemistry, fluidics, mechanics, photonics, biology etc.) and state-of-the-art technologies (bottom-up and top-down fabrication for various hard and soft materials, precise fluidic control, single molecule detection methods, and particle surface modification methods etc.), which have not been not covered by conventional review papers or books. Therefore, researchers or students new to the field need a new book covering these fields including recent research topics, applications and problems to be solved in the future. Our motivation is to summarize the state-of-the-art technologies for research and demonstrate new chemistry and fluidics in extended-nano space for students and researchers in academia or industry. We also emphasize the potential large impact microfluidic technologies have on chemistry and biochemistry.
Author: Daniel Warner Trahan
Publisher:
ISBN:
Category :
Languages : en
Pages : 115
Get Book Here
Book Description
During most of the twentieth century, direct study of individual polymer molecules was impossible due to their small size. Therefore, polymers were typically studied in bulk solutions, and their behavior and interactions had to be understood through average bulk property measurements. Because the scale of most industrial applications greatly exceeded the size of these molecules, this level of analysis was satisfactory. In the last twenty years, however, the appearance of microfluidic devices, whose smallest length scales are comparable to the size of a polymer molecule, has offered ways to visually study the behavior of individual polymer molecules and made possible new and exciting applications that exploit the precise control afforded by the small size of these devices. One such application is gene mapping, which extracts, at a. coarse level, the information embedded in the base pair sequence of genomic DNA. This technology relies on the ability to manipulate single DNA molecules in order to perform such tasks as separating DNA based on length and stretching DNA away from its entropically coiled equilibrium state. Recently, many novel methods have been proposed to accomplish these tasks using microfabricated devices, and munch experimental work has been focused on identifying and characterizing the underlying physics governing these devices. Current understanding, however, is greatly hampered by the fact that experiments can only provide limited information about the behavior of DNA molecules (e.g., they are unable to resolve details on small time and length scales). Therefore, simulations are an invaluable tool in the study of DNA behavior in microfiuidic devices by complementing and guiding experimental investigations. In this thesis, we present Brownian Dynamics simulations of the single molecule behavior of DNA in microfluidic devices related to gene mapping. In particular, we have considered the use of a post array to "precondition" the configuration of molecules for subsequent stretching in a contraction and compared our results to previous experiments. We found good qualitative agreement between experiments and simulations for DNA behavior in the post array, but our simulations consistently overpredicted the final stretch of molecules at the end of the contraction, which we attributed to nonlinear electrokinetic effects. We also investigate the electrophoretic collision of a DNA molecule with a. large, ideally conducting post. Field-induced compression was shown to play a critical role in the escape process of a molecule trapped on the post surface, and an extensive theoretical analysis is performed, describing both the local field-induced compression and the larger collision problem. Finally, we study the relaxation process of an initially stretched molecule in slit-like confinement. We present the first simulation results that exhibit two distinct relaxation times in the linear force regime, as previously reported in recent experiments. Our analysis is focused on the experimentally inaccessible dynamics in the transverse directions, particularly at short times and on small length scales. Comparisons to the predictions of a recent mechanistic model of confined relaxation were found to be satisfactory.
Author: Nerayo Petros Teclemariam
Publisher:
ISBN:
Category :
Languages : en
Pages : 374
Get Book Here
Book Description
Author: Xin Hu
Publisher:
ISBN:
Category : Brownian movements
Languages : en
Pages : 180
Get Book Here
Book Description
Abstract: The dynamics of single DNA molecules in micro/nanofluidics has attracted a great deal of attention due to its importance in the biomedical applications such as DNA separation, gene mapping, and gene therapy. The conformation change of these single long chain molecules in different hydrodynamic flows (pure extensional and simple shear flows) was first observed with the fluorescence microscopy in experiments. The Brownian dynamics simulation was then carried out and it successfully explained the interesting phenomena in experimental observation. In this dissertation, two major flows (i.e., generated by either the hydrodynamic or electrokinetic forces) to control the DNA dynamics are thoroughly investigated. The main effort is concentrated on the electrokinetic micro-flows produced by different microfluidic patterns. The finite element method is used to simulate the electrokinetic flows and the solutions are used as inputs for the coarse-grained Brownian dynamics simulation, which can capture the dynamics of single DNA molecules. In the electrokinetic flows, the electroosmotic and electrophoretic interactions affect the flow patterns of charged particles. When the electrophoretic mobility of a charged particle is higher than the surface electroosmotic mobility, the flows in the microfluidic devices are essentially the electrophoresis-dominated extensional flows even with surface charge patterning. To avoid this limitation, a novel design of a five-cross microfluidic device is proposed based on simulations. This design can generate and maintain different particle flow patterns even when the electrophoretic mobility is much higher than the electroosmotic mobility. Different responses of single DNA molecules under various hydrodynamic and electrokinetic flows are also studied. The complicated DNA molecule is simplified as either a bead-spring or a bead-rod chain in the Brownian dynamics simulations. Different forces in the governing equation of a bead-spring or bead-rod chain are discussed thoroughly. Different time-marching schemes are used and compared in the simulation of the dynamics of single DNA molecules. The simulations of DNA dynamics in the hydrodynamic and electrokinetic flows agree well with the experiments and the previous simulation results.
Author: Shengnian Wang
Publisher:
ISBN:
Category : Electrokinetics
Languages : en
Pages : 248
Get Book Here
Book Description
Abstract: The purpose of this study is to fabricate polymer nanodevices and investigate micro-/nanofluidic and DNA dynamics in non-uniform electrokinetic flows. Single DNA dynamic deformation was firstly studied in cross-slot microfluidic platforms. Three basic flow patterns (i.e., extensional, shear and rotational flows) were generated and polystyrene nanospheres were used to identify the flow characteristics. The conformational evolution of lambda-DNA molecules was also investigated and it indicated that the initial conformation of molecules and their residence time in the flow play important roles in the dynamic of DNA stretching. A new nanonozzle array was developed by Sacrificial Template Imprinting (STI), which can provide more uniform and controllable DNA stretching compared to the cross-slot design. A polymer sacrificial template was used to avoid structure damage or defects during the de-molding process. It was produced by a two-step replication, starting with a conically shaped nanotip array. Each nanonozzle is 3 micrometer high with the size at the small end down to 80 nm. In conjuction with surface modification and silica synthesis on the surface, the channel size was further reduced and the polymer structure was reinforced. Further 2D dynamic complexation exhibited a two-stage complexation, extending gradually from the small end towards the large end. Nanonozzle can provide two important flow patterns: the converging flow and the diverging flow. 2D converging microchannels were used to investigate the migration behavior of rigid nanospheres and flexible DNA molecules. Vortices were observed both inside and at the small end of the converging channel. When nanoparticles have much smaller size than the channels (i.e., the hindered factor is small), for example, in dynamic assembly, non-uniform surface charge led to the formation of new vortex pair. The stagnation region between the double vortex pairs is believed to have the primary complexation. When hindered factor is large, hindered migration was shown in the diverging direction, while nanonozzles were easily clogged in the converging direction for rigid colloid nanospheres. But for flexible lambda-DNA molecules, their molecular chain can be stretched to achieve easy pass in the converging direction even though their equilibrium size are much larger than the channel size.
Author: Gilead Henkin
Publisher:
ISBN:
Category :
Languages : en
Pages :
Get Book Here
Book Description
"As biological physics develops at the level of single-molecule studies, new tools are required to make observations at micrometer and nanometer scales. Many methods have been developed in the past decade offering unprecedented control and resolution at these lengthscales - for example, novel fluorescent techniques such as fluorescence resonance energy transfer (FRET) probe nanoscale interactions, and optical tweezers or atomic force microscopy (AFM) measure forces with exquisite sensitivity. However, the ability to simultaneously control and measure molecules at this scale remains a great challenge, often requiring physical restraint of molecules on a surface or other limiting modifications. Towards realizing new experimental regimes allowing control and measurement of less-constrained molecules, we present a novel micro- and nanofluidic device for controlling and visualizing DNA interactions. A previously-developed method for confining DNA into nanochannels, Convex Lens-induced Confinement (CLiC), is modified with fabricated structures allowing dynamic exchange of small reagents during confinement. Use of this device is demonstrated through a series of vignette experiments. First, we present a series of experiments examining the interaction between DNA complexed with the fluorescent dye YOYO-1 and buffer solutions with varying ionic strength. We find evidence for different fluorescent binding modes by simultaneously measuring polymer length and fluorescent intensity as the molecules react to the ionic strength change in real time. We follow with short preliminary studies observing dynamic compaction by a cationic surfactant and cleavage by several restriction enzymes. We then present an application of this device in which we use a binding agent to selectively deposit stretched DNA onto a silica surface, potentially useful for examining DNA samples under higher-resolution microscopes such as AFM or SEM." --
