Author: Tracy Lanise Johnson
Publisher:
ISBN:
Category :
Languages : en
Pages : 400
Book Description
RNA Binding to RNA Polymerase II Purified from the Yeast Saccharomyces Cerevisiae
Author: Tracy Lanise Johnson
Publisher:
ISBN:
Category :
Languages : en
Pages : 400
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 400
Book Description
Characterization of an RNA Polymerase II Subunit, RPB9, and a Transcript Elongation Factor, TFIIS, from Saccharomyces Cerevisiae
Author: Rodney Gerard Weilbaecher
Publisher:
ISBN:
Category :
Languages : en
Pages : 536
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 536
Book Description
Analysis of RNA Polymerase II Transcription Initiation in the Yeast Saccharomyces Cerevisiae
Author: Rachel Imoberdorf
Publisher:
ISBN:
Category :
Languages : en
Pages : 130
Book Description
Transcription occurs in a repressive chromatin context. Chromatin modifications are therefore often required to allow transcriptional activation. Here, we addressed the question whether global histone acetylation is involved in the regulation of transcriptional activation of class II promoters. We show that global histone acetylation has a role in activation of RNA polymerase II dependent transcription by modulating the inherent inhibitory effects of chromatin on the formation of the pre-initiation complex. In a second project we have developed a chromatin immunoprecipitation based assay that allows the study of transcriptional reinitiation "in vivo". Our results suggest the formation of a reinitiation scaffold on the GAL1, but not the ACT1 promoter. In a third project assessed whether TFIIB reaches promoters in the form of the holoenzyme "in vivo". Our data indicate that TFIIB does not assemble in a holoenzyme but associates with another, yet unidentified, factor(s) prior to promoter binding.
Publisher:
ISBN:
Category :
Languages : en
Pages : 130
Book Description
Transcription occurs in a repressive chromatin context. Chromatin modifications are therefore often required to allow transcriptional activation. Here, we addressed the question whether global histone acetylation is involved in the regulation of transcriptional activation of class II promoters. We show that global histone acetylation has a role in activation of RNA polymerase II dependent transcription by modulating the inherent inhibitory effects of chromatin on the formation of the pre-initiation complex. In a second project we have developed a chromatin immunoprecipitation based assay that allows the study of transcriptional reinitiation "in vivo". Our results suggest the formation of a reinitiation scaffold on the GAL1, but not the ACT1 promoter. In a third project assessed whether TFIIB reaches promoters in the form of the holoenzyme "in vivo". Our data indicate that TFIIB does not assemble in a holoenzyme but associates with another, yet unidentified, factor(s) prior to promoter binding.
Structural Features of Yeast RNA Polymerase II Via Electron Crystallography
Author: Gavin David Meredith
Publisher:
ISBN:
Category :
Languages : en
Pages : 310
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 310
Book Description
Regulation of RNA Polymerase II Transcription by the SPT Proteins in Yeast Saccharomyces Cerevisiae
Author: Lei Zhang
Publisher:
ISBN:
Category : Messenger RNA.
Languages : en
Pages : 288
Book Description
Publisher:
ISBN:
Category : Messenger RNA.
Languages : en
Pages : 288
Book Description
Regulation of RNA Polymerase I-dependent Transcription by the Ribosomal DNA Enhancer in Saccharomyces Cerevisiae
Author: John Jun Kang
Publisher:
ISBN:
Category :
Languages : en
Pages : 444
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 444
Book Description
Functional Studies of RNA Polymerase II-dependent Transcription in Yeast Saccharomyces Cerevisiae
Author: Susanna Tronnersjö
Publisher:
ISBN: 9789157672582
Category :
Languages : en
Pages : 68
Book Description
Publisher:
ISBN: 9789157672582
Category :
Languages : en
Pages : 68
Book Description
Biochemical and Genetic Characterization of the Transcription Elongation Factor TFIIS from the Yeast Saccharomyces Cerevisiae
Author: Karen Renee Christie
Publisher:
ISBN:
Category :
Languages : en
Pages : 600
Book Description
Regulation of the process of transcriptional elongation is an important control mechanism in the expression of some genes. To fully understand this form of regulation will require better understanding of the functions of transcription elongation factors. The goal of this work was to characterize the transcription elongation factor TFIIS from Saccharomyces cerevisiae, originally called P37. I demonstrated that, like the mammalian TFIIS proteins, the yeast protein stimulates RNA polymerase II to cleave the nascent RNA transcript and to read-through an intrinsic block to elongation. Investigation of the protein-protein contacts between TFIIS and RNA polymerase II indicated that the carboxyl-terminal domain of the largest subunit, subunit four, and subunit seven of the polymerase are not required for TFIIS to promote cleavage and read-through by the polymerase. In addition the carboxyl-terminal half of the yeast TFIIS protein is sufficient for both of these in vitro activities. This result is consistent with the previous results demonstrating the carboxyl-terminus of mouse TFIIS was sufficient to activate RNA polymerase in vitro.
Publisher:
ISBN:
Category :
Languages : en
Pages : 600
Book Description
Regulation of the process of transcriptional elongation is an important control mechanism in the expression of some genes. To fully understand this form of regulation will require better understanding of the functions of transcription elongation factors. The goal of this work was to characterize the transcription elongation factor TFIIS from Saccharomyces cerevisiae, originally called P37. I demonstrated that, like the mammalian TFIIS proteins, the yeast protein stimulates RNA polymerase II to cleave the nascent RNA transcript and to read-through an intrinsic block to elongation. Investigation of the protein-protein contacts between TFIIS and RNA polymerase II indicated that the carboxyl-terminal domain of the largest subunit, subunit four, and subunit seven of the polymerase are not required for TFIIS to promote cleavage and read-through by the polymerase. In addition the carboxyl-terminal half of the yeast TFIIS protein is sufficient for both of these in vitro activities. This result is consistent with the previous results demonstrating the carboxyl-terminus of mouse TFIIS was sufficient to activate RNA polymerase in vitro.
Mechanism and Regulation of Yeast RNA Polymerase II Transcription Initiation and Termination
Author: Jason Nicholas Kuehner
Publisher:
ISBN:
Category :
Languages : en
Pages : 188
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 188
Book Description
Methods in Yeast Genetics
Author: David C. Amberg
Publisher: CSHL Press
ISBN: 0879697288
Category : Genetics
Languages : en
Pages : 250
Book Description
"Methods in Yeast Genetics" is a course that has been offered annually at Cold Spring Harbor for the last 30 years. This provides a set of teaching experiments along with the protocols and recipes for the standard techniques and reagents used in the study of yeast biology.
Publisher: CSHL Press
ISBN: 0879697288
Category : Genetics
Languages : en
Pages : 250
Book Description
"Methods in Yeast Genetics" is a course that has been offered annually at Cold Spring Harbor for the last 30 years. This provides a set of teaching experiments along with the protocols and recipes for the standard techniques and reagents used in the study of yeast biology.