Ribosomes Structure, Function, and Dynamics PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Ribosomes Structure, Function, and Dynamics PDF full book. Access full book title Ribosomes Structure, Function, and Dynamics by Marina V. Rodnina. Download full books in PDF and EPUB format.
Author: Marina V. Rodnina
Publisher: Springer Science & Business Media
ISBN: 3709102154
Category : Medical
Languages : en
Pages : 428
Get Book Here
Book Description
The ribosome is a macromolecular machine that synthesizes proteins with a high degree of speed and accuracy. Our present understanding of its structure, function and dynamics is the result of six decades of research. This book collects over 40 articles based on the talks presented at the 2010 Ribosome Meeting, held in Orvieto, Italy, covering all facets of the structure and function of the ribosome. New high-resolution crystal structures of functional ribosome complexes and cryo-EM structures of translating ribosomes are presented, while partial reactions of translation are examined in structural and mechanistic detail, featuring translocation as a most dynamic process. Mechanisms of initiation, both in bacterial and eukaryotic systems, translation termination, and novel details of the functions of the respective factors are described. Structure and interactions of the nascent peptide within, and emerging from, the ribosomal peptide exit tunnel are addressed in several articles. Structural and single-molecule studies reveal a picture of the ribosome exhibiting the energy landscape of a processive Brownian machine. The collection provides up-to-date reviews which will serve as a source of essential information for years to come.
Author: Marina V. Rodnina
Publisher: Springer Science & Business Media
ISBN: 3709102154
Category : Medical
Languages : en
Pages : 428
Get Book Here
Book Description
The ribosome is a macromolecular machine that synthesizes proteins with a high degree of speed and accuracy. Our present understanding of its structure, function and dynamics is the result of six decades of research. This book collects over 40 articles based on the talks presented at the 2010 Ribosome Meeting, held in Orvieto, Italy, covering all facets of the structure and function of the ribosome. New high-resolution crystal structures of functional ribosome complexes and cryo-EM structures of translating ribosomes are presented, while partial reactions of translation are examined in structural and mechanistic detail, featuring translocation as a most dynamic process. Mechanisms of initiation, both in bacterial and eukaryotic systems, translation termination, and novel details of the functions of the respective factors are described. Structure and interactions of the nascent peptide within, and emerging from, the ribosomal peptide exit tunnel are addressed in several articles. Structural and single-molecule studies reveal a picture of the ribosome exhibiting the energy landscape of a processive Brownian machine. The collection provides up-to-date reviews which will serve as a source of essential information for years to come.
Author: Colin Echeverría Aitken
Publisher:
ISBN:
Category :
Languages : en
Pages : 216
Get Book Here
Book Description
Author: Michael B. Mathews
Publisher:
ISBN: 9781621821861
Category : Genetic translation
Languages : en
Pages : 499
Get Book Here
Book Description
A subject collection from Cold Spring Harbor Perspectives in Biology.
Author: Heena Sharma
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
Get Book Here
Book Description
Ribosome dynamics play an essential role in orchestrating all stages of protein synthesis. Recent biochemical, structural and computational studies have shown large-scale conformational changes of the ribosome, its tRNA substrates and translation factors during the elongation phase of protein synthesis. Dynamic movements of the ribosome not only govern the translation process but are also targeted by many antibiotics resulting in inhibition of protein synthesis. Therefore, complete understanding of conformational rearrangements in the ribosome will improve our knowledge about translation me...
Author: Greco Hernández
Publisher: Springer
ISBN: 3319394681
Category : Science
Languages : en
Pages : 564
Get Book Here
Book Description
The “omics” era has given a new perspective to the findings on the origin and evolution of the process of translation. This book provides insight into the evolution of the translation process and machinery from a modern perspective. Written by leading experts in molecular biology, this text looks into the origins and evolution of the protein synthetic machinery.
Author: Wei Ning
Publisher:
ISBN:
Category :
Languages : en
Pages :
Get Book Here
Book Description
Protein synthesis, one of nature's most fundamental processes within all living cells, is catalyzed by the ribosome, a highly conserved, massive, two-subunit ribonucleoprotein complex. Ribosomes synthesize proteins based on the sequence of triplet-nucleotide codons presented by the messenger RNA (mRNA) template, using aminoacyl-transfer RNAs (aa-tRNAs) substrates, which deliver individual amino acids to the ribosome. Recent biochemical, structural, dynamic and computational studies have uncovered large-scale conformational changes of the ribosome, its tRNA substrates, and translation factors that play important roles in regulating protein synthesis, especially during the elongation phase of translation. For example, translocation of the ribosome along its mRNA template involves several conformational rearrangements of the ribosomal pre-translocation (PRE) complex, including the rotation of two ribosomal subunits, closure of the L1 stalk element, and reconfigurations of the ribosome-bound tRNAs.
Author: Ron Milo
Publisher: Garland Science
ISBN: 1317230698
Category : Science
Languages : en
Pages : 400
Get Book Here
Book Description
A Top 25 CHOICE 2016 Title, and recipient of the CHOICE Outstanding Academic Title (OAT) Award. How much energy is released in ATP hydrolysis? How many mRNAs are in a cell? How genetically similar are two random people? What is faster, transcription or translation?Cell Biology by the Numbers explores these questions and dozens of others provid
Author: Alexander Spirin
Publisher: Springer Science & Business Media
ISBN: 1461578175
Category : Science
Languages : en
Pages : 417
Get Book Here
Book Description
This book is based on an advanced course of lectures on ribosome structure and protein biosynthesis that I offer at the Moscow State University. These lectures have been part of a general course on molecular biology for almost three decades, and they have undergone considerable evolution as knowledge has been pro gressing in this field. The progress continues, and readers should be prepared that some facts, statements, and ideas included in the book may be incomplete or out of-date. In any case, this is primarily a textbook, but not a comprehensive review. It provides a background of knowledge and current ideas in the field and gives ex amples of observations and their interpretations. I understand that some interpre tations and generalizations may be tentative or disputable, but I hope that this will stimulate thinking and discussing better than if I left white spots. The book has a prototype: it is my monograph "Ribosome Structure and Pro tein Biosynthesis" published by the Benjamin/Cummings Publishing Company, Menlo Park, California, in 1986. Here I have basically kept the former order of pre sentation ofthe topics and the subdivision into chapters. The contents ofthe chap ters, however, have been significantly revised and supplemented. The newly writ ten chapters on translational control in prokaryotes (Chapter 16) and eukaryotes (Chapter 17) are added.
Author: Sabine Müller
Publisher: John Wiley & Sons
ISBN: 3527814531
Category : Science
Languages : en
Pages : 81
Get Book Here
Book Description
Ribozymes Provides comprehensive coverage of a core field in the molecular biosciences, bringing together decades of knowledge from the world’s top professionals in the field Timely and unique in its breadth of content, this all-encompassing and authoritative reference on ribozymes documents the great diversity of nucleic acid-based catalysis. It integrates the knowledge gained over the past 35 years in the field and features contributions from virtually every leading expert on the subject. Ribozymes is organized into six major parts. It starts by describing general principles and strategies of nucleic acid catalysis. It then introduces naturally occurring ribozymes and includes the search for new catalytic motifs or novel genomic locations of known motifs. Next, it covers the development and design of engineered ribozymes, before moving on to DNAzymes as a close relative of ribozymes. The next part examines the use of ribozymes for medicinal and environmental diagnostics, as well as for therapeutic tools. It finishes with a look at the tools and methods in ribozyme research, including the techniques and assays for structural and functional characterization of nucleic acid catalysts. The first reference to tie together all aspects of the multi-faceted field of ribozymes Features more than 30 comprehensive chapters in two volumes Covers the chemical principles of RNA catalysis; naturally occurring ribozymes, engineered ribozymes; DNAzymes; ribozymes as tools in diagnostics and therapy, and tools and methods to study ribozymes Includes first-hand accounts of concepts, techniques, and applications by a team of top international experts from leading academic institutions Dedicates half of its content to methods and practical applications, ranging from bioanalytical tools to medical diagnostics to therapeutics Ribozymes is an unmatched resource for all biochemists, biotechnologists, molecular biologists, and bioengineers interested in the topic.
Author: Peiwu Qin
Publisher:
ISBN:
Category : Electronic dissertations
Languages : en
Pages : 189
Get Book Here
Book Description
The Ribosome is the protein factory with multiple coordinated components working together to translate the genetic code in the mRNA into protein sequence. Since it was discovered in 1955, the research around the ribosome has been pushed forward by hundreds of labs worldwide. In 2000, the breakthrough was marked by the publication of the ribosome structures at atomic resolution. Structures help people design and explain the biochemical data deeper and better. However, the ribosome is not static. It is a dynamic and highly regulated machine. The function of the ribosome can be understood further only if we can follow the dynamics of individual components in different functional states. Solution NMR is a powerful technique for studying protein dynamics. However, the gigantic nature of the ribosome makes this task daunting. Thanks to the development of single molecule techniques, the first real time ribosome dynamics was done in 2004 by Scott Blanchard when he was a post-doctorate with Steven Chu and Joseph Puglisi. After that, more than 300 research papers and reviews have been published about singe molecule ribosome studies. Both single molecule FRET and optical tweezers have been successfully used to address the dynamic process of protein translation. In 2008 and 2009, Dr. Peter Cornish and Dr. Dmitri Ermolenko followed the ribosome intersubunit rotation and L1 stalk dynamics in real time during the process of translocation, which was the first direct evidence of ribosome dynamics itself since the previous study inferred the ribosome dynamics from tRNA motion. The previous study could not exclude the possibility that the observed dynamics resulted purely from tRNA. Cornish and Ermolenko concluded that ribosome dynamics is a spontaneous process that is driven by thermodynamic Brownian motion. This pioneering study opened a window to address many unresolved problems such as the perturbation of the dynamic effects by structured RNA, mRNA unwinding, and ribosomal frameshifting. We found that the presence of RNA structure induces the ribosome into a new FRET state that we named super rotated state. The population distribution of the super rotated state is correlated with the thermostability and the distance of RNA structure to the ribosome. We can reduce the population of the super rotated state and reverse this change. Using other RNA structures like DNA:RNA hybrid and a pseudoknot, the ribosome can also be induced into the super rotated state. The different global conformations of ribosome when linear or structured RNA are present are shown by small angle X-ray scattering. The changes in the pair distance distribution function indicate the rotational change of ribosome theoretically. Structured RNA inhibits the regular intersubunit rotation and drives the ribosome into the super rotated state. However, the structured RNA cannot stop the open-to-close transition of the L1 stalk, which still can fluctuate between three different functional states. These results propose that ribosome dynamics is composed of several independent units with their own identity. Since the super rotated state also can be induced by DNA:RNA hybrid, we can investigate how far the RNA structure is away from the ribosome when the ribosome can sense the presence of these RNA structures.
