Revealing the Interactions of RNA Polymerase II Binding Proteins PDF Download
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Author: Roland Becker
Publisher:
ISBN:
Category :
Languages : en
Pages : 123
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Book Description
Author: Roland Becker
Publisher:
ISBN:
Category :
Languages : en
Pages : 123
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Book Description
Author: Chirlmin Joo
Publisher: Springer Nature
ISBN: 1493997262
Category : Science
Languages : en
Pages : 249
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Book Description
RNA molecules play key roles in all aspects of cellular life, but to do so efficiently, they must work in synergism with proteins. This book addresses how proteins and RNA interact to carry out biological functions such as protein synthesis, regulation of gene expression, genome defense, liquid phase separation and more. The topics addressed in this volume will appeal to researchers in biophysics, biochemistry and structural biology. The book is a useful resource for anybody interested in elucidating the molecular mechanisms and discrete properties of RNA-protein complexes. Included are reviews of key systems such as microRNA and CRISPR/Cas that exemplify how RNA and proteins work together to perform their biological function. Also covered are techniques ranging from single molecule fluorescence and force spectroscopy to crystallography, cryo-EM microscopy, and kinetic modeling.
Author: Christopher W.J. Smith
Publisher: Oxford University Press, UK
ISBN: 0191591629
Category :
Languages : en
Pages : 370
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Book Description
RNA-protein interactions play a fundamental role in gene expression and protein synthesis. Recent research into the role of RNA in cells has elucidated many more vital interactions with proteins. This book provides an up-to-date and comprehensive guide to a wide range of laboratory procedures to investigate the interactions between RNA and proteins. - ;RNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly catalytic action of the ribosome in protein synthesis, are established as being fundamental to the function of RNA. Recent research into, for example, the role of RNA as a catalyst, has elucidated many more interactions with proteins that are vital to cell function. RNA - Protein Interactions: A Practical Approach provides a clear and comprehensive guide to the experimental procedures used in studying RNA - protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction, various biochemical and genetic approaches to purifying and cloning RNA binding proteins, through to methods for an in depth analysis of the structural basis of the interaction. The volume includes a number of procedures that have not previously been covered in this type of manual. These include the production of site-specifically modified RNAs by enzymatic and chemical methods and in vivo screening for novel RNA - protein interactions in yeast and E. coli . This is the first volume to gather in one place this wide array of approaches for studying RNA - protein interactions. As is customary for the Practical Approach series, the writing is characterized by a clear explanatory style with many detailed protocols. This informative book will be a valuable aid to laboratory workers in biochemistry and molecular biology - graduate students, postdoctoral and senior scientists - whose research encompasses this field. -
Author: Kiyoshi Nagai
Publisher: Oxford University Press, USA
ISBN:
Category : Medical
Languages : en
Pages : 302
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Book Description
The study of RNA-protein interactions is crucial to understanding the mechanisms and control of gene expression and protein synthesis. The realization that RNAs are often far more biologically active than was previously appreciated has stimulated a great deal of new research in this field. Uniquely, in this book, the world's leading researchers have collaborated to produce a comprehensive and current review of RNA-protein interactions for all scientists working in this area. Timely, comprehensive, and authoritative, this new Frontiers title will be invaluable for all researchers in molecular biology, biochemistry and structural biology.
Author: Frank J. Schmidt
Publisher: Humana Press
ISBN: 9781493918959
Category : Medical
Languages : en
Pages : 0
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Book Description
In this volume expert researchers in the field detail many of the methods which are now commonly used to study RNA. These methods are presented as a guidebook to scientists who are experienced with RNA research and want to brush up on a new technique. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Thorough and intuitive, RNA-RNA Interactions: Methods and Protocols guides scientists investigating biological systems and studying RNA.
Author: Kenneth Berendzen
Publisher: Bentham Science Publishers
ISBN: 1608054926
Category : Science
Languages : en
Pages : 225
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Book Description
A major goal of integrative research is understanding regulatory networks to such an extent as to allow researchers to model developmental and stress responses. Regulatory networks of living systems include complex and vast interactions between proteins, metabolites, RNA, various signaling molecules and DNA. One aspect of systems biology is understanding the dynamics of protein-DNA interactions affecting gene expression that are caused by transcription factors (TFs) and chromatin remodeling factors. This e-book provides a resource for summarizing current knowledge eukaryotic transcription and explores cis-elements and methods for their analysis, prediction and discovery. The book also presents an overview of exploring gene regulatory networks, chromatin, and miRNAs. Information about state-of-the-art techniques for the determination of TF - cis-element interactions in vivo and in silico give cutting edge insights on how genomic-scale research is being approached. The Analysis of Regulatory DNA provides readers with both the necessary background knowledge and provocative, up-to-date insights aimed at sparking new and vibrant experimental designs for understanding and predicting cis-elements in the eukaryotic genome.
Author: Stephanie C. Huelga
Publisher:
ISBN: 9781303990236
Category :
Languages : en
Pages : 221
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Book Description
Nascent transcripts produced by RNA polymerase II in eukaryotic cells are subject to extensive processing prior to the generation of a functional messenger RNA (mRNA). These RNAs are generally found coated with RNA binding proteins (RBPs), which act in concert to regulate RNA processing events, including splicing, polyadenylation, and RNA stability. The aggregate effect of various RBPs on a given RNA transcript eventually dictates its fate, a phenomenon referred to as the RNP code. The precise control of RNA processing by RBPs is incredibly important for cellular homeostasis, defects of which lead to numerous accounts of human genetic diseases in many tissues. With the emergence of genome-wide methods for detecting direct binding of RBPs on target RNAs, coupled with technologies to measure changes in various aspects of RNA processing, global rules and insights for individual RBPs have been revealed. However, few studies have combined regulatory changes for more than one RBP to better understand their combinatorial effects on RNA targets. Additionally, despite the importance of RBPs, we still do not have the complete repertoire of which proteins bind to RNA, and which of these bind simultaneously with other RBPs to constitute the "RBP-RNA interactome". Here, I conduct genome-wide RNA processing analyses in mammalian cells, integrating regulation and binding information for multiple RBPs, including disease-related RBPs, the highly abundant heterogeneous nuclear ribonucleoparticle (hnRNP) proteins, and many previously uncharacterized RBPs. This battery of computational and experimental assays provides insight into the unique roles of hundreds of individual RBPs, as well as the extent of coordinated regulation between RBPs. I also describe a systematic approach to identifying the proteins that interact with RNA simultaneously with hnRNP proteins. Not limited to only mRNA-bound proteins, my strategy identifies thousands of hnRNP protein interactors, including putative novel proteins that interact with mRNA and pre-mRNA, and have biochemical and statistical attributes of known RBPs. My findings expand the repertoire of RNA-interacting RBPs and provide a resource for the study of human simultaneous RBP-RBP interactomes. This comprehensive analysis of RBPs investigates their specific roles in the regulation of RNA processing yielding interesting findings for the RNA biology field and insights into how misregulation can impact human disease.
Author: Ke-li Han
Publisher: Springer Science & Business Media
ISBN: 3319029703
Category : Medical
Languages : en
Pages : 488
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Book Description
This book discusses how biological molecules exert their function and regulate biological processes, with a clear focus on how conformational dynamics of proteins are critical in this respect. In the last decade, the advancements in computational biology, nuclear magnetic resonance including paramagnetic relaxation enhancement, and fluorescence-based ensemble/single-molecule techniques have shown that biological molecules (proteins, DNAs and RNAs) fluctuate under equilibrium conditions. The conformational and energetic spaces that these fluctuations explore likely contain active conformations that are critical for their function. More interestingly, these fluctuations can respond actively to external cues, which introduces layers of tight regulation on the biological processes that they dictate. A growing number of studies have suggested that conformational dynamics of proteins govern their role in regulating biological functions, examples of this regulation can be found in signal transduction, molecular recognition, apoptosis, protein / ion / other molecules translocation and gene expression. On the experimental side, the technical advances have offered deep insights into the conformational motions of a number of proteins. These studies greatly enrich our knowledge of the interplay between structure and function. On the theoretical side, novel approaches and detailed computational simulations have provided powerful tools in the study of enzyme catalysis, protein / drug design, protein / ion / other molecule translocation and protein folding/aggregation, to name but a few. This work contains detailed information, not only on the conformational motions of biological systems, but also on the potential governing forces of conformational dynamics (transient interactions, chemical and physical origins, thermodynamic properties). New developments in computational simulations will greatly enhance our understanding of how these molecules function in various biological events.
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Book Description
Author: Mary Sopta
Publisher:
ISBN:
Category :
Languages : en
Pages : 374
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Book Description
