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Author: Edna Christine Hardeman
Publisher:
ISBN:
Category : Enzyme inhibitors
Languages : en
Pages : 384
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Book Description
Author: Edna Christine Hardeman
Publisher:
ISBN:
Category : Enzyme inhibitors
Languages : en
Pages : 384
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Book Description
Author: David Gordon Skalnik
Publisher:
ISBN:
Category :
Languages : en
Pages : 292
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Book Description
Author: Deborah Ann Brown
Publisher:
ISBN:
Category :
Languages : en
Pages : 426
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Book Description
Author: Benjamin Preiss
Publisher: Elsevier
ISBN: 0323155146
Category : Nature
Languages : en
Pages : 343
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Book Description
Regulation of HMG-CoA Reductase is a nine-chapter text that focuses on the research developments in the regulation of HMG-CoA reductase enzyme and cholesterol biosynthesis. This book deals first with the role of cholesterol in the regulation of its own biosynthesis and the work involving compactin and related competitive inhibitors of HMG-CoA reductase. The subsequent chapters examine the reversible phosphorylation of HMG-CoA reductase in hormonal and other short-term physiological changes and the structure and properties of the enzyme, including evidence for modulation of enzyme activity and structure unrelated to phosphorylation. These topics are followed by discussions on the regulation of reductase in the biosynthesis of ubiquinone, dolichols, and isopentenyl-tRNA and the regulation of reductase activity in extrahepatic tissues. This text further explores the contributions made through the use of cultured cells and their genetic manipulation, with emphasis on the great potential of this approach. The concluding chapters address the status of the low-density lipoprotein pathway and related mechanisms. These chapters also consider the regulation of human hepatic HMG-CoA reductase and the special problems inherent in work with human tissues. Enzyme scientists and researchers and graduate students will find this book invaluable.
Author: G.H.Neil Towers
Publisher: Springer Science & Business Media
ISBN: 1468487892
Category : Science
Languages : en
Pages : 349
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Book Description
This series of lectures was delivered at the 29th meeting of the Phytochemical Society of North America, held at the University of British Columbia in Vancouver, B. C. , Canada on June 16th-20th, 1989. Topics concerning terpenoids, consisting of isoprene units, are now so numerous that a judicious selection for a relatively limited symposium was difficult. We were able to assemble, however, a potpourri of reviews on topical areas of terpenoid chemistry, biochemistry and biology, by scientists who are making exciting contributions and whose work points the way to significant future research. Because of the importance of terpenoids in the life of plants, and indeed in all living organisms, a periodical review of the mevalonic acid pathway and of the subsequent biochemical events leading to the biosynthesis of isoprenoids needs no justification. Life, as we know it, would not be possible without the ability of living organisms to employ this metabolic sequence which proceeds from condensations of three molecules of acetyl-CoA and terminates with the elaboration of the terpenoid precursors, isopentenyl pyrophosphate and dimethylallyl pyrophosphate. In addition to producing obviously essential compounds that are partially or completely of isoprenoid origin (Fig. 1), such as hormones, photosynthetic pigments, compounds involved in electron transport in respiration and in photosynthesis, oxidative enzymes and membrane components, plants elaborate thousands of novel terpenoids, many of which do not as yet have identifiable physiological, biochemical or even ecological roles, e. g. the cardenolides, ecdysones or saponins.
Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1796
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Book Description
Author: David Kritchevsky
Publisher: Springer Science & Business Media
ISBN: 1461324599
Category : Medical
Languages : en
Pages : 503
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Book Description
The Proceedings of the Eight International Symposium on Drugs Affecting Lipid Metabolism (8th D.A.L.M.) is the subject of this volume. Since the first symposium in 1960, each successive meeting has broken new ground in the field of pharmacological control of lipid levels - offering new and stimulating insights and exposing the audience to the state of the art. The field has progressed sufficiently to permit discussion of the cellular biology of athero sclerosis. The opening session was devoted to pathology, macrophages, lipoproteins and their receptors and choles terol ester metabolism. Because of the recent emergence of new apolipoprotein technology, a workshop devoted sole ly to apolipoprotein methodology was introduced followed by a plenary session devoted to their metabolism and structure. Another rapidly developing area of atherosclerosis research is non-invasive assessment of this condition. Accordingly, a session was devoted to new techniques for this research modality. The final plenary sessions were devoted to the roles of drugs and diet in athero~ scl~rosis - cause, treatment and mechanisms of action. The meeting was summarized by Dr. O.J. Pollak, one of the "founding fathers" of this field. There were nine sessions of proffered papers whose abstracts appear in this volume. In addition, special workshops (to be reported elsewhere) were devoted to several drugs including Oiyzanol, Probucol and Etofibrate.
Author:
Publisher:
ISBN:
Category : Electronic journals
Languages : en
Pages : 810
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Author: Thomas Edward Meigs
Publisher:
ISBN:
Category :
Languages : en
Pages : 478
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Author: William Engfelt
Publisher:
ISBN:
Category : Cell lines
Languages : en
Pages : 440
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Book Description
3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase is present not only in the endoplasmic reticulum (ER) but also in the peroxisomes. To date no information is available regarding the function of the peroxisomal HMG-CoA reductase in cholesterol/isoprenoid metabolism and the structure of the peroxisomal HMG-CoA reductase has yet to be determined. We have identified a mammalian cell line that expresses only one HMG-CoA reductase protein and which is localized exclusively to peroxisomes to facilitate our studies on the function, regulation, and structure of the peroxisomal HMG-CoA reductase. This cell line was obtained by growing UT2 cells (which lack the ER HMG-CoA reductase), in the absence of mevalonate. The surviving cells exhibited a marked increase in a 90 kDa HMG-CoA reductase which was localized exclusively to peroxisomes. The UT2 cells grown in the absence of mevalonate containing the upregulated peroxisomal HMG-CoA reductase are designated UT2*. A detailed characterization and analysis of this cell line is presented in this study. The examination of UT2/UT2* cell cDNA and genomic DNA has led to the identification of two novel point mutations in intronic sequences of the ER HMG-CoA reductase gene. One mutation identified at the +1 position (G → A) of the 5 ′ splice site of exon 11-12 junction was shown to cause exon 11 skipping which resulted in the insertion of premature stop codons. A second mutation was also identified at the +5 position (G → A) of the 5′ splice site in the intron spanning exons 13 and 14. Furthermore, the data indicate that the two mutations in the reductase gene are present on the same allele. As demonstrated by RT-PCR of UT2 cell mRNA, the mutations produce aberrant spliced messages. If the aberrant messages were translated, truncated proteins of 44 kDa or 66 kDa would be predicted. More importantly, these truncated proteins would be expected not to have catalytic activity. Thus, the mutations identified in the ER reductase gene in UT2/UT2* cells indicate that neither a 97 kDa nor a 90 kDa reductase protein can be produced from this gene.
