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Author: Murat Sunbul
Publisher: Humana
ISBN: 9781493995394
Category : Science
Languages : en
Pages : 231
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Book Description
This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity labeling methods, the volume continues with BioID, proximity labeling methods that describe the proximity ligation assay to detect RNA-DNA interactions, UV cross-linking to demonstrate RNA-protein interactions, and how chemical and enzymatic reactivities can be improved upon DNA-DNA and protein-protein interactions, as well as “proximity-induced self-labeling,” where the radius of labeling is zero. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Proximity Labeling: Methods and Protocols serves as an ideal guide for researchers exploring the crucial roles that proximity-driven reactions play in biological systems.
Author: Murat Sunbul
Publisher: Humana
ISBN: 9781493995394
Category : Science
Languages : en
Pages : 231
Get Book Here
Book Description
This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity labeling methods, the volume continues with BioID, proximity labeling methods that describe the proximity ligation assay to detect RNA-DNA interactions, UV cross-linking to demonstrate RNA-protein interactions, and how chemical and enzymatic reactivities can be improved upon DNA-DNA and protein-protein interactions, as well as “proximity-induced self-labeling,” where the radius of labeling is zero. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Proximity Labeling: Methods and Protocols serves as an ideal guide for researchers exploring the crucial roles that proximity-driven reactions play in biological systems.
Author: Gregory R. Hancock
Publisher: Routledge
ISBN: 1317627792
Category : Education
Languages : en
Pages : 515
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Book Description
The Reviewer’s Guide to Quantitative Methods in the Social Sciences provides evaluators of research manuscripts and proposals in the social and behavioral sciences with the resources they need to read, understand, and assess quantitative work. 35 uniquely structured chapters cover both traditional and emerging methods of quantitative data analysis, which neither junior nor veteran reviewers can be expected to know in detail. The second edition of this valuable resource updates readers on each technique’s key principles, appropriate usage, underlying assumptions and limitations, providing reviewers with the information they need to offer constructive commentary on works they evaluate. Written by methodological and applied scholars, this volume is also an indispensable author’s reference for preparing sound research manuscripts and proposals.
Author: Lindsay Groves
Publisher: Springer
ISBN: 3642412025
Category : Computers
Languages : en
Pages : 479
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Book Description
This book constitutes the refereed proceedings of the 15th International Conference on Formal Engineering Methods, ICFEM 2013, held in Queenstown, New Zealand, in October/November 2013. The 28 revised full papers together with 2 keynote speeches presented were carefully reviewed and selected from 88 submissions. The topics covered are abstraction and refinement, formal specification and modeling, program analysis, software verification, formal methods for software safety, security, reliability and dependability, tool development, integration and experiments involving verified systems, formal methods used in certifying products under international standards, and formal model-based development and code generation.
Author: Gary R. Rassel
Publisher: Taylor & Francis
ISBN: 1000816443
Category : Social Science
Languages : en
Pages : 401
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Book Description
Practical Research Methods for Nonprofit and Public Administrators, Second Edition, covers basic principles of research design, data collection, data analysis, reporting and ethical principles of research. The discussion describes the skills as they would be used in practice in a not-for-profit or public administration environment. The book gives pragmatic instructions for designing research to ensure that results will be accurate and administrators can have confidence in them. It covers techniques to assure that researchers are measuring what they intend to measure and in a way that will be useful and manageable. Sampling methods to find out about larger populations are discussed as are survey, interview, and focus group data collection procedures. The book describes how Geographic Information Systems (GIS) are used to obtain information on the locations of service needs and providers and to draw maps showing these. Participatory research methods including community needs assessments and asset mapping are discussed. The book includes chapters on statistical analysis, visual displays of data, reporting of results and evaluation of programs. The use of computer programs for analyzing data is detailed and illustrated by examples. The appropriate software for analysis and mapping is discussed. A chapter updates the ethics and government requirements for research. Each chapter includes examples and exercises to enhance learning. The book is intended for administrators in not-for-profit and public administration organizations, providing practical skills that can be used in many situations. It is also intended for use as a text in academic programs in administration and management.
Author: John R. Hayes
Publisher: Routledge
ISBN: 1136465138
Category : Education
Languages : en
Pages : 377
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Book Description
This unique volume returns in its second edition, revised and updated with the latest advances in problem solving research. It is designed to provide readers with skills that will make them better problem solvers and to give up-to-date information about the psychology of problem solving. Professor Hayes provides students and professionals with practical, tested methods of defining, representing, and solving problems. Each discussion of the important aspects of human problem solving is supported by the most current research on the psychology problem solving. The Complete Problem Solver, Second Edition features: *Valuable learning strategies; *Decision making methods; *Discussions of the nature of creativity and invention, and *A new chapter on writing. The Complete Problem Solver utilizes numerous examples, diagrams, illustrations, and charts to help any reader become better at problem solving. See the order form for the answer to the problem below.
Author: Radoslaw Pytlak
Publisher: Springer
ISBN: 3540486623
Category : Science
Languages : en
Pages : 224
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Book Description
While optimality conditions for optimal control problems with state constraints have been extensively investigated in the literature the results pertaining to numerical methods are relatively scarce. This book fills the gap by providing a family of new methods. Among others, a novel convergence analysis of optimal control algorithms is introduced. The analysis refers to the topology of relaxed controls only to a limited degree and makes little use of Lagrange multipliers corresponding to state constraints. This approach enables the author to provide global convergence analysis of first order and superlinearly convergent second order methods. Further, the implementation aspects of the methods developed in the book are presented and discussed. The results concerning ordinary differential equations are then extended to control problems described by differential-algebraic equations in a comprehensive way for the first time in the literature.
Author: TianQiao Liu
Publisher: Springer Nature
ISBN: 9819715148
Category :
Languages : en
Pages : 581
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Book Description
Author: D. L. Lorenz
Publisher:
ISBN:
Category : Stream measurements
Languages : en
Pages : 52
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Book Description
Author:
Publisher:
ISBN:
Category : Hydrology
Languages : en
Pages : 52
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Book Description
Author: Nicholas P. Cheremisinoff
Publisher: CRC Press
ISBN: 9780824780210
Category : Technology & Engineering
Languages : en
Pages : 1408
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Book Description
