Protein/Peptide Sequence Analysis: Current Methodologies

Protein/Peptide Sequence Analysis: Current Methodologies PDF Author: A.S. Bhown
Publisher: CRC Press
ISBN: 1351092987
Category : Medical
Languages : en
Pages : 348

Get Book Here

Book Description
This book is an attempt to provide in a single source current state-of-the-art methodologies for protein sequence analysis. It is hoped that these various chapters are presented in such a way that both the newcomer and the established protein chemist will find useful information and directions to new techniques. This book offers a rich array of techniques and methods for sequencing proteins and peptides. It should meet the expectations of investigators in protein chemistry who wish to update their knowledge of sequencing techniques, and of those who wish to reacquaint themselves with the best available current technologies.

Protein/Peptide Sequence Analysis: Current Methodologies

Protein/Peptide Sequence Analysis: Current Methodologies PDF Author: A.S. Bhown
Publisher: CRC Press
ISBN: 1351092987
Category : Medical
Languages : en
Pages : 348

Get Book Here

Book Description
This book is an attempt to provide in a single source current state-of-the-art methodologies for protein sequence analysis. It is hoped that these various chapters are presented in such a way that both the newcomer and the established protein chemist will find useful information and directions to new techniques. This book offers a rich array of techniques and methods for sequencing proteins and peptides. It should meet the expectations of investigators in protein chemistry who wish to update their knowledge of sequencing techniques, and of those who wish to reacquaint themselves with the best available current technologies.

Methods in Protein Sequence Analysis

Methods in Protein Sequence Analysis PDF Author: Brigitte Wittmann-Liebold
Publisher: Springer Science & Business Media
ISBN: 3642738346
Category : Science
Languages : en
Pages : 608

Get Book Here

Book Description
"Methods in Protein Sequence Analysis - 1988" - contains selected contributions on modern protein- analytical techniques as presented by speakers at the Seventh International Conference on "Methods in Protein Sequence Analysis", held from July 3rd to July 8th, 1988 in Berlin. The book contains information on new methodologies for sensitive amino acid analysis, N- and C-terminal sequence analysis, and protein and peptide purification. In addition recent mass spectrometric approaches are described, as an alter native technique to the common stepwise degradative sequence analysis of polypeptides by the Edman method. The book presents new possibilities in the design of sequencers and sophisticated equipment for the structural analysis of peptides and proteins. It describes practical approaches for the investigation of protein domains and protein complexes, and contains review chapters on the crystallization of cell organelles as well as on recent theoretical aspects of protein folding mechanisms. The nature of protein folding is not yet understood, but further advances in this area would greatly enhance our present knowledge of protein structure and function. Further, the book gives examples of the application of gene technology to protein characterization and to the design of new proteins. This enables new studies on the structure and function of proteins to be made, and opens up efficient approaches to the design of drugs.

Proteomics in Functional Genomics

Proteomics in Functional Genomics PDF Author: P. Jolles
Publisher: Birkhäuser
ISBN: 3034884583
Category : Science
Languages : en
Pages : 240

Get Book Here

Book Description
A wealth of information has accumulated over the last few years on the human genome. The new insights have completely changed the focus of protein analysis. It is no longer time-consuming analysis of unknown products, but rather selective identifications of individual forms, modifications and processings, and overall analysis of global protein outputs from cells and tissues in health and disease. This book gears to the rising need of sensitive, accurate, and fast separation and identification techniques in proteomics. It discusses current methodologies of modern protein analysis, from isolation and sample preparation, over analysis and identification, to final characterization. Several evaluations concentrate on the now productive approaches of two-dimensional gel electrophoresis and mass spectrometry, but alternative methods and further perspectives are also outlined. The book includes an overlook over current databases to connect protein analysis data with all available information,...

Methods in Peptide and Protein Sequence Analysis

Methods in Peptide and Protein Sequence Analysis PDF Author: Christian Birr
Publisher:
ISBN:
Category :
Languages : en
Pages :

Get Book Here

Book Description


Methods in Protein Sequence Analysis

Methods in Protein Sequence Analysis PDF Author: Marshall Elzinga
Publisher: Springer Science & Business Media
ISBN: 1461258324
Category : Science
Languages : en
Pages : 581

Get Book Here

Book Description
Methods in Protein Sequence Analysis contains an intensely prac tical account of all the new methodology available to scientists carrying out protein and peptide sequencing studies. Many of the striking advances in fields as diverse as immunology, cell motility, and neurochemistry have in fact been fueled by our ever more powerful ability to determine the sequences and structures of key proteins and peptides. It is our hope that the rich array of tech niques and methods for sequencing proteins discussed in this volume-methods that generate much of the information crucial to progress in modern biology-will now become accessible to all who can benefit from them. The papers of the present volume constitute the Proceedings of the IVth International Conference on Methods in Protein Se quence Analysis, which was held at Brookhaven National Labo ratory, Upton, NY, September 21-25, 1981. It was the most recent in a series of biennial conferences, the previous one having been held in Heidelberg, GFR, in 1979. The series was originated by Richard Laursen, and initially dealt with one aspect of the field, solid-phase sequencing. The scope of the meeting was very broad and among the many aspects of protein sequencing discussed were: instrumentation, strategy, chemicals, mass spectrometry, cleavage of proteins and separation of peptides, and solid, liquid, manual, and even "gas phase" sequencing.

A Practical Guide to Protein and Peptide Purification for Microsequencing

A Practical Guide to Protein and Peptide Purification for Microsequencing PDF Author: Paul T. Matsudaira
Publisher: Elsevier
ISBN: 0080924611
Category : Science
Languages : en
Pages : 205

Get Book Here

Book Description
Why a Second Edition?The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. - Mass spectrophotometric characterization of proteins and peptides - N-terminal sequencing of proteins with blocked N termini - Internal amino acid sequence analysis after protease digestion in-gel and on-blot - Improved microscale peptide purification methods - Computer analysis of protein sequences - New protocols tested and refined through everyday use in authors' laboratories - Updated reference chapter covering all aspects of protein microsequencing

Techniques in Protein Chemistry

Techniques in Protein Chemistry PDF Author: John W. Crabb
Publisher: Elsevier
ISBN: 1483217590
Category : Science
Languages : en
Pages : 591

Get Book Here

Book Description
Techniques in Protein Chemistry V highlights current methods in peptide and protein mass spectrometry, sequence and amino acid analysis, fragmentations, separations, protein folding and modeling, peptide and protein NMR, and peptide synthesis. This volume emerged from the manuscripts presented at the Seventh Symposium of the Protein Society, held in San Diego on July 24-28, 1993. This volume is organized into eight parts encompassing 61 chapters. The first part surveys the peptide and protein characterization, detection, and analysis by mass spectrometry. The subsequent parts describe the structural characterization and analysis of posttranslational processing events, as well as the characterization of protein and amino acid sequences using several analytical techniques. Other parts explore other analytical methods for peptide and protein separations; some aspects involved in protein design and functional domain analysis; and the evaluation of protein conformation, folding, and modeling. The last parts contain research papers on NMR analysis of peptide and protein solution structures. These parts also look into topics related to peptide synthesis and peptide libraries. This book is intended primarily for protein and analytical chemists.

Protein Sequencing Protocols

Protein Sequencing Protocols PDF Author: Bryan John Smith
Publisher: Springer Science & Business Media
ISBN: 1592593429
Category : Science
Languages : en
Pages : 489

Get Book Here

Book Description
Determination of the protein sequence is as important today as it was a half century ago, even though the techniques and purposes have changed over time. Mass spectrometry has continued its recent rapid development to find notable application in the characterization of small amounts of protein, for example, in the field of proteomics. The “traditional” chemical N-terminal sequencing is still of great value in quality assurance of the increasing number of biopharmaceuticals that are to be found in the clinic, checking processing events of recombinant proteins, and so on. It is joined in the armory of me- ods of protein analysis by such techniques as C-terminal sequencing and amino acid analysis. These methods are continually developing. The first edition of Protein Sequencing Protocols was a “snapshot” of methods in use in protein biochemistry laboratories at the time, and this, the second edition, is likewise. Methods have evolved in the intervening period, and the content of this book has similarly changed, the content of some chapters having been superceded and replaced by other approaches. Thus, in this edition, there is inclusion of approaches to validation of methods for quality assurance work, reflecting the current importance of biopharmaceuticals, and also a guide to further analysis of protein sequence information, acknowledging the importance of bioinformatics.

Protein/Peptide Sequence Analysis: Current Methodologies

Protein/Peptide Sequence Analysis: Current Methodologies PDF Author: A.S. Bhown
Publisher: CRC Press
ISBN: 1351084534
Category : Medical
Languages : en
Pages : 251

Get Book Here

Book Description
This book is an attempt to provide in a single source current state-of-the-art methodologies for protein sequence analysis. It is hoped that these various chapters are presented in such a way that both the newcomer and the established protein chemist will find useful information and directions to new techniques. This book offers a rich array of techniques and methods for sequencing proteins and peptides. It should meet the expectations of investigators in protein chemistry who wish to update their knowledge of sequencing techniques, and of those who wish to reacquaint themselves with the best available current technologies.

Methods in Peptide and Protein Sequence Analysis

Methods in Peptide and Protein Sequence Analysis PDF Author: Christian Birr
Publisher: Elsevier-North-Holland Biomedical Press
ISBN:
Category : Science
Languages : en
Pages : 568

Get Book Here

Book Description
Nineteen-year-old Sarah masquerades as a man during the Civil War, serving as a nurse on the battlefield and a spy for the Union Army, escaping from the Confederates, and falling in love with one of her fellow soldiers. Based on the life of Sarah Emma Edmonds.