Protein Dynamics Using NMR Relaxation PDF Download
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Author: Kevin H. Mayo
Publisher:
ISBN: 9781860940767
Category : Science
Languages : en
Pages : 300
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Book Description
This book specifically addresses the use of (13)C and (15)N NMR spectroscopy to derive information on the internal motions of proteins and peptides in solution. Basic methods of NMR relaxation, along with the general theory of correlation functions and spectral densities, are first covered, followed by in-depth analyses and comparisons of various motional models and molecular dynamics simulations used to derive protein dynamics information from NMR relaxation experiments. Motional model equations are expressed in computer program-ready format. The text is ideally suited for graduate and post-graduate students as well as experienced professionals.
Author: Kevin H. Mayo
Publisher:
ISBN: 9781860940767
Category : Science
Languages : en
Pages : 300
Get Book Here
Book Description
This book specifically addresses the use of (13)C and (15)N NMR spectroscopy to derive information on the internal motions of proteins and peptides in solution. Basic methods of NMR relaxation, along with the general theory of correlation functions and spectral densities, are first covered, followed by in-depth analyses and comparisons of various motional models and molecular dynamics simulations used to derive protein dynamics information from NMR relaxation experiments. Motional model equations are expressed in computer program-ready format. The text is ideally suited for graduate and post-graduate students as well as experienced professionals.
Author: Martin Joseph Dellwo
Publisher:
ISBN:
Category :
Languages : en
Pages : 392
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Book Description
Author: 靜瑜·周
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: A.Joshua Wand
Publisher: Academic Press
ISBN: 9780128138601
Category : Science
Languages : en
Pages : 0
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Book Description
Biological NMR, Part A, the latest release in the Methods of Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Protein methyl labeling, Membrane protein expression - yeast, Protein aromatic labeling, His-tag/Metal contamination, Bicelles, nanodiscs and micelles MP host, PTM - phosphorylation, PTM - lipidation, Screening platform for receptor-ligand discovery Solution Spectroscopy, Large protein strategies, NUS data collection/analysis, F19 incl. hydration, ODNP - hydration, Reverse micelle - Hydration Solid State Spectroscopy, SS NMR membrane proteins, SS NMR soluble/aggregate proteins, SS DNP - general, SS NMR nucleic acids, Structure determination and computer analysis, and much more.
Author: Danqing Jin
Publisher:
ISBN:
Category :
Languages : en
Pages : 122
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Book Description
Author: Alexandra Ahlner
Publisher: Linköping University Electronic Press
ISBN: 9175191032
Category : Nuclear magnetic resonance spectroscopy
Languages : en
Pages : 79
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Book Description
Proteins are essential for all known forms of life and in many lethal diseases protein failure is the cause of the disease. To understand proteins and the processes they are involved in, it is valuable to know their structures as well as their dynamics and interactions. The structures may not be directly inspected because proteins are too small to be visible in a light microscope, which is why indirect methods such as nuclear magnetic resonance (NMR) spectroscopy have to be utilized. This method provides atomic information about the protein and, in contrast to other methods with similar resolution, the measurements are performed in solution resulting in more physiological conditions, enabling analysis of dynamics. Important dynamical processes are the ones on the millisecond timeframe, which may contribute to interactions of proteins and their catalysis of chemical reactions, both of significant value for the function of the proteins. To better understand proteins, not only do we need to study them, but also develop the methods we are using. This thesis presents four papers about improved NMR techniques as well as a fifth where the kinase domain of ephrinB receptor 2 (EphB2) has been studied regarding the importance of millisecond dynamics and interactions for the activation process. The first paper presents the software COMPASS, which combines statistics and the calculation power of a computer with the flexibility and experience of the user to facilitate and speed up the process of assigning NMR signals to the atoms in the protein. The computer program PINT has been developed for easier and faster evaluation of NMR experiments, such as those that evaluate protein dynamics. It is especially helpful for NMR signals that are difficult to distinguish, so called overlapped peaks, and the soft- ware also converts the detected signals to the indirectly measured physical quantities, such as relaxation rate constants, principal for dynamics. Next are two new versions of the Carr-Purcell-Maiboom-Gill (CPMG) dispersion pulse sequences, designed to measure millisecond dynamics in a way so that the signals are more separated than in standard experiments, to reduce problems with overlaps. To speed up the collection time of the data set, a subset is collected and the entire data set is then reconstructed, by multi-dimensional decomposition co-processing. Described in the thesis is also a way to produce suitably labeled proteins, to detect millisecond dynamics at C? positions in proteins, using the CPMG dispersion relaxation experiment at lower protein concentrations. Lastly, the kinase domain of EphB2 is shown to be more dynamic on the millisecond time scale as well as more prone to interact with itself in the active form than in the inactive one. This is important for the receptor function of the protein, when and how it mediates signals. To conclude, this work has extended the possibilities to study protein dynamics by NMR spectroscopy and contributed to increased understanding of the activation process of EphB2 and its signaling mechanism.
Author: Edward James d'Auvergne
Publisher:
ISBN:
Category : Estimation theory
Languages : en
Pages : 353
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Book Description
Author: Klaartje Houben
Publisher:
ISBN: 9789039337806
Category :
Languages : en
Pages : 128
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Book Description
Author: Nabarun Chakraborty
Publisher:
ISBN:
Category :
Languages : en
Pages : 52
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Book Description
Author: John L. Markley
Publisher: Oxford University Press
ISBN: 0195357426
Category : Science
Languages : en
Pages : 375
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Book Description
This book presents a critical assessment of progress on the use of nuclear magnetic resonance spectroscopy to determine the structure of proteins, including brief reviews of the history of the field along with coverage of current clinical and in vivo applications. The book, in honor of Oleg Jardetsky, one of the pioneers of the field, is edited by two of the most highly respected investigators using NMR, and features contributions by most of the leading workers in the field. It will be valued as a landmark publication that presents the state-of-the-art perspectives regarding one of today's most important technologies.
