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Author: Nevan J. Krogan, PhD
Publisher: Springer
ISBN: 3319236032
Category : Science
Languages : en
Pages : 326
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Book Description
This book focuses on innovative experimental and computational approaches for charting interaction networks in bacterial species. The first part of the volume consists of nine chapters, focusing on biochemical and genetics and genomics approaches including yeast two hybrid, metagenomics, affinity purification in combination with mass spectrometry, chromatin-immunoprecipitation coupled with sequencing, large-scale synthetic genetic screens, and quantitative-based mass spectrometry strategies for mapping the bacterial physical, functional, substrate, and regulatory interaction networks needed for interpreting biological networks, inferring gene function, enzyme discovery, and identifying new drug targets. The second part comprises five chapters, covering the network of participants for protein folding and complex enzyme maturation. It also covers the structural approaches required to understand bacterial intramembrane proteolysis and the structure and function of bacterial proteins involved in surface polysaccharides, outer membrane, and envelope assembly. This volume concludes with a focus on computational and comparative genomics approaches, especially network-based methods for predicting physical or functional interactions, and integrative analytical approaches for generating more reliable information on bacterial gene function. This book provides foundational knowledge in the understanding of prokaryotic systems biology by illuminating how bacterial genes f unction within the framework of global cellular processes. The book will enable the microbiology community to create substantive resources for addressing many pending unanswered questions, and facilitate the development of new technologies that can be applied to other bacterial species lacking experimental data.
Author: Nevan J. Krogan, PhD
Publisher: Springer
ISBN: 3319236032
Category : Science
Languages : en
Pages : 326
Get Book Here
Book Description
This book focuses on innovative experimental and computational approaches for charting interaction networks in bacterial species. The first part of the volume consists of nine chapters, focusing on biochemical and genetics and genomics approaches including yeast two hybrid, metagenomics, affinity purification in combination with mass spectrometry, chromatin-immunoprecipitation coupled with sequencing, large-scale synthetic genetic screens, and quantitative-based mass spectrometry strategies for mapping the bacterial physical, functional, substrate, and regulatory interaction networks needed for interpreting biological networks, inferring gene function, enzyme discovery, and identifying new drug targets. The second part comprises five chapters, covering the network of participants for protein folding and complex enzyme maturation. It also covers the structural approaches required to understand bacterial intramembrane proteolysis and the structure and function of bacterial proteins involved in surface polysaccharides, outer membrane, and envelope assembly. This volume concludes with a focus on computational and comparative genomics approaches, especially network-based methods for predicting physical or functional interactions, and integrative analytical approaches for generating more reliable information on bacterial gene function. This book provides foundational knowledge in the understanding of prokaryotic systems biology by illuminating how bacterial genes f unction within the framework of global cellular processes. The book will enable the microbiology community to create substantive resources for addressing many pending unanswered questions, and facilitate the development of new technologies that can be applied to other bacterial species lacking experimental data.
Author: Simon Baumberg
Publisher: OUP Oxford
ISBN: 0191565571
Category : Science
Languages : en
Pages : 350
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Book Description
Prokaryotic gene expression is not only of theoretical interest but also of highly practical significance. It has implications for other biological problems, such as developmental biology and cancer, brings insights into genetic engineering and expression systems, and has consequences for important aspects of applied research. For example, the molecular basis of bacterial pathogenicity has implications for new antibiotics and in crop development. Prokaryotic Gene Expression is a major review of the subject, providing up-to-date coverage as well as numerous insights by the prestigious authors. Topics covered include operons; protein recognition of sequence specific DNA- and RNA-binding sites; promoters; sigma factors, and variant tRNA polymerases; repressors and activators; post-transcriptional control and attenuation; ribonuclease activity, mRNA stability, and translational repression; prokaryotic DNA topology, topoisomerases, and gene expression; regulatory networks, regulatory cascades and signal transduction; phosphotransfer reactions; switch systems, transcriptional and translational modulation, methylation, and recombination mechanisms; pathogenicity, toxin regulation and virulence determinants; sporulation and genetic regulation of antibiotic production; origins of regulatory molecules, selective pressures and evolution of prokaryotic regulatory mechanisms systems. Over 1100 references to the primary literature are cited. Prokaryotic Gene Expression is a comprehensive and authoritative review of current knowledge and research in the area. It is essential reading for postgraduates and researchers in the field. Advanced undergraduates in biochemistry, molecular biology, and microbiology will also find this book useful.
Author: Ali Navid
Publisher: Humana Press
ISBN: 9781617798269
Category : Science
Languages : en
Pages : 0
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Book Description
Systems biology is the study of interactions between assorted components of biological systems with the aim of acquiring new insights into how organisms function and respond to different stimuli. Although more and more efforts are being directed toward examining systems biology in complex multi-cellular organisms, the bulk of system-level analyses conducted to date have focused on the biology of microbes. In, Microbial Systems Biology: Methods and Protocols expert researchers in the field describe the utility and attributes of different tools (both experimental and computational) that are used for studying microbial systems. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Systems Biology: Methods and Protocols introduces and aids scientists in using the various tools that are currently available for analysis, modification and utilization of microbial organisms.
Author: Mohamed Al-Rubeai
Publisher: Springer Science & Business Media
ISBN: 1402052529
Category : Science
Languages : en
Pages : 426
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Book Description
This book is a comprehensive guide to the revolutionary area of systems biology and its application in cell culture engineering. It is designed to offer a state-of-the-art review with in depth assessments and perspectives of post-genomic biology through understanding the molecular and cellular basis of integrated biological systems. The chapters describe the necessary methodologies for performing systems biology research.
Author: Joseph W. Lengeler
Publisher: Wiley-Blackwell
ISBN: 9780632053575
Category : Science
Languages : en
Pages : 998
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Book Description
Designed as an upper-level textbook and a reference for researchers, this important book concentrates on central concepts of the bacterial lifestyle. Taking a refreshingly new approach, it present an integrated view of the prokaryotic cell as an organism and as a member of an interacting population. Beginning with a description of cellular structures, the text proceeds through metabolic pathways and metabolic reactions to the genes and regulatory mechanisms. At a higher level of complexity, a discussion of cell differentiation processes is followed by a description of the diversity of prokaryotes and their role in the biosphere. A closing section deals with man and microbes (ie, applied microbiology). The first text to adopt an integrated view of the prokaryotic cell as an organism and as a member of a population. Vividly illustrates the diversity of the prokaryotic world - nearly all the metabolic diversity in living organisms is found in microbes. New developments in applied microbiology highlighted. Extensive linking between related topics allows easy navigation through the book. Essential definitions and conclusions highlighted. Supplementary information in boxes.
Author:
Publisher: Academic Press
ISBN: 0323901476
Category : Science
Languages : en
Pages : 462
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Book Description
Recombinant Protein Expression, Part A, Volume 659 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more. Additional sections cover the Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins, Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry, Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli, Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambr® 250, and Expression of recombinant antibodies in Leishmania tarentolae. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology serial Updated release includes the latest information on Recombinant Protein Expression
Author: Jan Löwe
Publisher: Springer
ISBN: 331953047X
Category : Science
Languages : en
Pages : 457
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Book Description
This book describes the structures and functions of active protein filaments, found in bacteria and archaea, and now known to perform crucial roles in cell division and intra-cellular motility, as well as being essential for controlling cell shape and growth. These roles are possible because the cytoskeletal and cytomotive filaments provide long range order from small subunits. Studies of these filaments are therefore of central importance to understanding prokaryotic cell biology. The wide variation in subunit and polymer structure and its relationship with the range of functions also provide important insights into cell evolution, including the emergence of eukaryotic cells. Individual chapters, written by leading researchers, review the great advances made in the past 20-25 years, and still ongoing, to discover the architectures, dynamics and roles of filaments found in relevant model organisms. Others describe one of the families of dynamic filaments found in many species. The most common types of filament are deeply related to eukaryotic cytoskeletal proteins, notably actin and tubulin that polymerise and depolymerise under the control of nucleotide hydrolysis. Related systems are found to perform a variety of roles, depending on the organisms. Surprisingly, prokaryotes all lack the molecular motors associated with eukaryotic F-actin and microtubules. Archaea, but not bacteria, also have active filaments related to the eukaryotic ESCRT system. Non-dynamic fibres, including intermediate filament-like structures, are known to occur in some bacteria.. Details of known filament structures are discussed and related to what has been established about their molecular mechanisms, including current controversies. The final chapter covers the use of some of these dynamic filaments in Systems Biology research. The level of information in all chapters is suitable both for active researchers and for advanced students in courses involving bacterial or archaeal physiology, molecular microbiology, structural cell biology, molecular motility or evolution. Chapter 3 of this book is open access under a CC BY 4.0 license.
Author: Hervé Tettelin
Publisher: Springer Nature
ISBN: 3030382818
Category : Science
Languages : en
Pages : 311
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Book Description
This open access book offers the first comprehensive account of the pan-genome concept and its manifold implications. The realization that the genetic repertoire of a biological species always encompasses more than the genome of each individual is one of the earliest examples of big data in biology that opened biology to the unbounded. The study of genetic variation observed within a species challenges existing views and has profound consequences for our understanding of the fundamental mechanisms underpinning bacterial biology and evolution. The underlying rationale extends well beyond the initial prokaryotic focus to all kingdoms of life and evolves into similar concepts for metagenomes, phenomes and epigenomes. The book’s respective chapters address a range of topics, from the serendipitous emergence of the pan-genome concept and its impacts on the fields of microbiology, vaccinology and antimicrobial resistance, to the study of microbial communities, bioinformatic applications and mathematical models that tie in with complex systems and economic theory. Given its scope, the book will appeal to a broad readership interested in population dynamics, evolutionary biology and genomics.
Author:
Publisher: Academic Press
ISBN: 0128201487
Category : Science
Languages : en
Pages : 360
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Book Description
Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems, Volume 638, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sample chapters from this new release include In vitro characterization of the colibactin-activating peptidase ClbP enables development of a fluorogenic activity probe, Using FDAA probes to study cell division in Bacillus subtilis, Chemoenzymatic synthesis of UDP-sugars, Chemical tools for selective activity profiling of bacterial penicillin-binding proteins, Chemical Probes Reveal and Extraseptal Mode of Cross-linking in Staphylococcus Aureus, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on retinoid signaling pathways
Author: Pat Vaughan
Publisher: Springer Science & Business Media
ISBN: 1592590683
Category : Science
Languages : en
Pages : 210
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Book Description
When setting out to decide on the content of DNA Repair Protocols: Prokaryotic Systems, I was conscious of the need to portray the vast array of pathways and enzymatic activities that are part of the discipline of DNA repair. In addition to the classical DNA repair activities, I wanted to convey the significant interest that has been generated in recent years in the use of the proteins and repair systems as research tools, much like the use of restriction enzymes over the last few decades. Therefore, in addition to chapters deta- ing protocols for investigating specific repair activities, I have included s- eral chapters in this book on the applied use of DNA repair proteins and systems. The many years of research on bacterial DNA repair systems have allowed us to really understand the majority of DNA repair pathways in bac- rial cells. Building on this knowledge, research has lead to major advances in understanding mammalian DNA repair and uncovered its links to human d- ease, such as DNA mismatch repair and colon cancer, nucleotide excision repair and xeroderma pigmentosum, DNA helicase function in Bloom’s s- drome, and so on. Such have been the advances that Science magazine iden- fied the collective DNA repair systems as its “Molecule of the Year” in 1994.
