Author: Ram J. Singh
Publisher: CRC Press
ISBN: 1351230743
Category : Science
Languages : en
Pages : 463
Get Book Here
Book Description
Earlier books on the handling of plant chromosomes have not included many of the innovations in cytological techniques for many important crops that have become available in recent years, including information on associating genes with chromosomes. The aim of this book is to compile all the plant cytogenetic techniques, previously published in earlier books, into a laboratory manual. The first part of the book describes standard cytological techniques that are routinely used by students. The second part covers methods used for specific crops for which common cytological methods do not work satisfactorily. The third part discusses cytogenetic techniques (cytology and genetics) for physically locating genes on specific chromosomes. This novel book will be highly useful to students, teachers, and researchers as it is a convenient and comprehensive reference for all plant cytogenetic techniques and protocols.
Author: Ram J. Singh
Publisher: CRC Press
ISBN: 1315356996
Category : Science
Languages : en
Pages : 544
Get Book Here
Book Description
Cytogenetics plays an important role in understanding the chromosomal and genetic architecture of plant species. Plant Cytogenetics, Third Edition follows the tradition of its predecessors presenting theoretical and practical aspects of plant cytogenetics. Chapters describe correct handling of plant chromosomes, methods in plant cytogenetics, cell division, reproduction methods, chromosome nomenclature, karyotype analysis, chromosomal aberrations, genome analysis, transgenic crops, and cytogenetics in plant breeding. This new edition begins with a brief introduction on the historical aspect of cytogenetics and flows directly into handling of plant chromosomes by classical and modern cytological techniques, classical Mendelian Genetics, brief description of cell division, and chromosome identification by karyotype analysis. The comprehension of cytogenetics is incomplete without information on the role of aneuploidy in associating a gene on a particular chromosome, and the book covers these methodologies as a primary topic. Covering classical to modern cytogenetics, the book presents to the reader the crucial role of cytogenetics in improving crops.
Author: K P S Malik
Publisher: New India Publishing
ISBN: 9387973999
Category : Medical
Languages : en
Pages : 6
Get Book Here
Book Description
The book is basically intended to accompany a course in cytogenetics students of Genetics and Plant Breeding . Students are presumed to have knowledge of basics in genetics, cytology and plant breeding but in the present book with the help of diagrams and explanations it has been attempted that even a beginner could grasp the core elements of the subject. The book has been strictly organized on the basis of course curriculum being taught in Universities. All the topics covered in the book have been ordered in a crisp and comprehensible manner avoiding complexities of a traditional textbook since it is a simply a guide book to supplement but not supplant the main texts.
Author: Melody S. Clark
Publisher: Springer Science & Business Media
ISBN: 3642878733
Category : Science
Languages : en
Pages : 551
Get Book Here
Book Description
Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.
Author: Tony Heitkam
Publisher: Springer Nature
ISBN: 1071632264
Category : Science
Languages : en
Pages : 563
Get Book Here
Book Description
This book provides an up-to-date account of the most widespread methods used by specialists in the field of plant cytogenetics and the emerging field of cytogenomics that will likely soon be adapted by more labs. From the classical basic karyological approaches to the most recent genomics-informed and computational methods, the volume explores genome size and ploidy level estimation, chromosome fixation, preparation, and manipulation, banding and staining techniques, in situ hybridization, as well as numerous methods that integrate cytogenetics with bioinformatics and computational genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Cytogenetics and Cytogenomics: Methods and Protocols serves as an ideal resource for plant scientists interested in molecular and evolutionary biology, breeding, systematics, and plant -omics in general.
Author: Marilyn S. Arsham
Publisher: John Wiley & Sons
ISBN: 1119061229
Category : Science
Languages : en
Pages : 1216
Get Book Here
Book Description
Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics. Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization (FISH) and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive description of the diagnostic tests offered by the clinical laboratory and explains the science behind them. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters by experts in the field. Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN’s cytogenetic language to describe chromosome changes; tips for laboratory management; examples of laboratory information systems; a collection of internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the basis of each of these procedures. This makes it a useful resource for researchers, clinicians, and lab professionals, as well as students in a university or medical school setting.
Author: Food and Agriculture Organization of the United Nations
Publisher: Food & Agriculture Org.
ISBN: 9251305269
Category : Technology & Engineering
Languages : en
Pages : 319
Get Book Here
Book Description
This paper provides guidelines for new high-throughput screening methods – both phenotypic and genotypic – to enable the detection of rare mutant traits, and reviews techniques for increasing the efficiency of crop mutation breeding.
Author: Oluf Gamborg
Publisher: Springer Science & Business Media
ISBN: 3642790488
Category : Science
Languages : en
Pages : 367
Get Book Here
Book Description
This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.
Author: Henry T. Nguyen
Publisher: Springer
ISBN: 3319641980
Category : Science
Languages : en
Pages : 216
Get Book Here
Book Description
This book examines the application of soybean genome sequences to comparative, structural, and functional genomics. Since the availability of the soybean genome sequence has revolutionized molecular research on this important crop species, the book also describes how the genome sequence has shaped research on transposon biology and applications for gene identification, tilling and positional gene cloning. Further, the book shows how the genome sequence influences research in the areas of genetic mapping, marker development, and genome-wide association mapping for identifying important trait genes and soybean breeding. In closing, the economic and botanical aspects of the soybean are also addressed.
Author: McKinsey L. Goodenberger
Publisher: Oxford University Press
ISBN: 0190604921
Category : Medical
Languages : en
Pages : 409
Get Book Here
Book Description
This is an essential manual for the future of genetic counselling. It codifies the theory and practice of laboratory genetic counselling in an accessible and comprehensive format. With contributions from laboratorians, geneticists, and genetic counsellors from more than 30 institutions, it offers a manual of standards and practices that will benefit students and counsellors at any career stage
