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Author: Robert N. Trigiano
Publisher: Routledge
ISBN: 1351424033
Category : Science
Languages : en
Pages : 476
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Book Description
Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research. The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include ""Micropropagation of Dieffenbachia,"" ""Micropropagation and in vitro flowering of rose,"" ""Propagation from nonmeristematic tissue-organogenesis,"" ""Variation in culture"" and ""Tissue culture of ferns.""It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science.
Author: Robert N. Trigiano
Publisher: Routledge
ISBN: 1351424033
Category : Science
Languages : en
Pages : 476
Get Book Here
Book Description
Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research. The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include ""Micropropagation of Dieffenbachia,"" ""Micropropagation and in vitro flowering of rose,"" ""Propagation from nonmeristematic tissue-organogenesis,"" ""Variation in culture"" and ""Tissue culture of ferns.""It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science.
Author: Mark T. Windham
Publisher: CRC Press
ISBN: 9780849310379
Category : Science
Languages : en
Pages : 432
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Book Description
Instructors, students and researchers in plant pathology have been searching for a primary text that combines an informal, easy-to-read style with a thorough introduction to the concepts and terminology of plant pathology. Plant Pathology Concepts and Laboratory Exercises answers their demand by presenting pathology principles, protocols and procedures, serving as a valuable resource tool for both students and researchers. This guide explains definitions of disease, characteristics of organisms that cause disease, and how diseases interact with hosts and the environment. Each topic is addressed by an expert in the field, and is supported by one or more lab exercises. The structure of the text allows for easy reading, with references minimized and major concepts highlighted at the beginning of each chapter. The laboratory exercises give added flexibility to instructors. There are experiments for both beginning and advanced students, and a broad choice of exercise topics that can be selected based upon the focus within each individual class. Step-by-step instructions are provided for each laboratory exercise.
Author: Robert N. Trigiano
Publisher: CRC Press
ISBN: 1439896143
Category : Medical
Languages : en
Pages : 608
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Book Description
Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnol
Author: Caula A. Beyl
Publisher: CRC Press
ISBN: 1466503882
Category : Science
Languages : en
Pages : 499
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Book Description
Includes a DVD Containing All Figures and Supplemental Images in PowerPoint This new edition of Plant Propagation Concepts and Laboratory Exercises presents a robust view of modern plant propagation practices such as vegetable grafting and micropropagation. Along with foundation knowledge in anatomy and plant physiology, the book takes a look into the future and how cutting edge research may impact plant propagation practices. The book emphasizes the principles of plant propagation applied in both temperate and tropical environments. In addition to presenting the fundamentals, the book features protocols and practices that students can apply in both laboratory and field experiences. The book shows readers how to choose the best methods for plant propagation including proper media and containers as well as performing techniques such as budding, cutting, layering, grafting, and cloning. It also discusses how to recognize and cope with various propagation challenges. Also included are concept chapters highlighting key information, laboratory exercises, anticipated laboratory results, stimulating questions, and a DVD containing all the figures in the book as well as some supplemental images.
Author: Robert H. Smith
Publisher: Academic Press
ISBN: 0323160476
Category : Science
Languages : en
Pages : 190
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Book Description
Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.
Author: Roberta H. Smith
Publisher: Academic Press
ISBN: 0124159206
Category : Science
Languages : en
Pages : 206
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Book Description
Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature
Author: Frank DiCosmo
Publisher: CRC Press
ISBN: 9780849351358
Category : Science
Languages : en
Pages : 244
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Book Description
Plant cell cultures are used extensively in studies of secondary metabolism, for the biosynthesis of pharmaceuticals, flavors, essences, and pigments. This book highlights recent developments in the in vitro growth of cultured plant cells and in the production of valuable secondary metabolites. Plant Cell Culture Secondary Metabolism details research on many exciting areas including:
Author: Jennie P. Mather
Publisher: Springer Science & Business Media
ISBN: 0585275718
Category : Science
Languages : en
Pages : 247
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Book Description
It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.
Author: Kiichi Fukui
Publisher: CRC Press
ISBN: 9780849389191
Category : Science
Languages : en
Pages : 298
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Book Description
Finally - a guide to cytological techniques written specifically for the plant chromosome researcher and student. Plant Chromosomes: Laboratory Methods thoroughly covers all important approaches to the study of plant chromosomes. It reviews each specific approach and describes requisite experimental techniques. These practical descriptions cover basic, standard techniques as well as the most recent research advances and state-of-the-art technologies. Plant Chromosomes: Laboratory Methods allows you to build on the knowledge of its expert authors, who have first-hand experience with the ins and outs of each approach. Through hundreds of trouble-shooting suggestions it also helps you avoid experimental pitfalls by providing invaluable tips at critical points in the experimental process. This book gives you the information you need to improve the power of your plant chromosome research - saving you time and effort in the process. No other single volume contains so much practical information on this topic.
Author: Robert N. Trigiano
Publisher: CRC Press
ISBN: 0203506561
Category : Science
Languages : en
Pages : 374
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Book Description
Biotechnology revolutionized traditional plant breeding programs. This rapid change produced new discussions on techniques and opportunities for commerce, as well as a fear of the unknown. Plant Development and Biotechnology addresses the major issues of the field, with chapters on broad topics written by specialists. The book applies an informal style that addresses the major aspects of development and biotechnology with minimal references, without sacrificing information or accuracy. Divided into five primary parts, this volume explores how the field emerged from its early theoretical base to the technical discipline of today. It also covers progress being made with genetically engineered plants, providing a snapshot of the field's controversial present. Part III discusses methods for preparing media, creating solutions and dilutions, and accomplishing sterile culture work. It investigates common methods for visualizing and documenting studies, and quantifying responses of tissue culture in research. Part IV delivers the essential foundation of plant tissue culture, introducing the three types of commonly used culture regeneration systems. Part V integrates propagation techniques with other methodologies for the modification and manipulation of germplasm. Part VI concludes with special sections. Subjects include in vitro plant pathology, recent research into genetic and phenotypic variation, the mechanics of commercial plant production, and the importance of clean cultures and problems associated with maintaining in vitro cultures. The final chapter analyzes entrepreneurship in the field and outlines the do's and don'ts to consider when launching an enterprise.
