Physicochemical Methods in the Study of Biomembranes PDF Download
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Author: Herwig J. Hilderson
Publisher: Springer Science & Business Media
ISBN: 1461518636
Category : Science
Languages : en
Pages : 524
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Book Description
In mammalian cells many physiological processes rely on the dynamics of the organization of lipids and proteins in biological membranes. The topics in this volume deal with physicochemical methods in the study of biomembranes. Some of them have a long and respectable history in the study of soluble proteins and have only recently been applied to the study of membranes. Some have tradi tionally been applied to studies of model systems of lipids of well-defined com position, as well as to intact membranes. Other methods, by their very nature, apply to organized bilayers comprised of both protein and lipid. Van Meer and van Genderen provide us with an introduction to the field (Chapter I). From their personal perspective regarding the distribution, trans port, and sorting of membrane lipids, they formulate a number of biologically relevant questions and show that the physicochemical methods described in this book may contribute in great measure to solving these issues. The methods of analytical ultracentrifugation have served faithfully for 60 years in the study of water-soluble proteins. The use of detergent extraction of membrane proteins, and the manipulation of density with H20/D20 mixtures, has extended this technique to the study of proteins, and in particular their interactions, from biological membranes. As described by Morris and Ralston in Chapter 2, this technique can be used to determine a number of important properties of proteins.
Author: Herwig J. Hilderson
Publisher: Springer Science & Business Media
ISBN: 1461518636
Category : Science
Languages : en
Pages : 524
Get Book Here
Book Description
In mammalian cells many physiological processes rely on the dynamics of the organization of lipids and proteins in biological membranes. The topics in this volume deal with physicochemical methods in the study of biomembranes. Some of them have a long and respectable history in the study of soluble proteins and have only recently been applied to the study of membranes. Some have tradi tionally been applied to studies of model systems of lipids of well-defined com position, as well as to intact membranes. Other methods, by their very nature, apply to organized bilayers comprised of both protein and lipid. Van Meer and van Genderen provide us with an introduction to the field (Chapter I). From their personal perspective regarding the distribution, trans port, and sorting of membrane lipids, they formulate a number of biologically relevant questions and show that the physicochemical methods described in this book may contribute in great measure to solving these issues. The methods of analytical ultracentrifugation have served faithfully for 60 years in the study of water-soluble proteins. The use of detergent extraction of membrane proteins, and the manipulation of density with H20/D20 mixtures, has extended this technique to the study of proteins, and in particular their interactions, from biological membranes. As described by Morris and Ralston in Chapter 2, this technique can be used to determine a number of important properties of proteins.
Author: Gertz I. Likhtenshtein
Publisher: Springer Nature
ISBN: 3030825035
Category : Science
Languages : en
Pages : 523
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Book Description
This book embraces all physiochemical aspects of the structure and molecular dynamics of water, focusing on its role in biological objects, e.g. living cells and tissue, and in the formation of functionally active structures of biological molecules and their ensembles. Water is the single most abundant chemical found in all living things. It offers a detailed look into the latest modern physical methods for studying the molecular structure and dynamics of the water and provides a critical analysis of the existing literature data on the properties of water in biological objects. Water as a chemical reagent and as a medium for the formation of conditions for enzymatic catalysis is a core focus of this book. Although well suited for active researchers, the book as a whole, as well as each chapter on its own, can be used as fundamental reference material for graduate and undergraduate students throughout chemistry, physics, biophysics and biomedicine.
Author: J. Katsaras
Publisher: Springer Science & Business Media
ISBN: 366204496X
Category : Science
Languages : en
Pages : 304
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Book Description
Provides the reader with an up to date insight of the current state of the art in the field of lipid bilayer research and the important insights derived for the understanding of the complex and varied behaviour of biological membranes and its function.
Author: Robert Bittman
Publisher: Springer Science & Business Media
ISBN: 1461559014
Category : Science
Languages : en
Pages : 567
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Book Description
Experts investigate the biochemical and biomedical aspects of cholesterol, addressing its metabolism in normal and disease states. They discuss a broad range of topics, including key steps in the cholesterol biosynthetic pathway, and the role of cholesterol in cancer, atherosclerosis, and diseases of the nervous system. The book's comprehensive coverage also includes the pathological consequences and potential therapies for various disease states, and the development of new anti-atherogenic drugs.
Author: Miguel A. R. B. Castanho
Publisher: Internat'l University Line
ISBN: 0972077456
Category : Medical
Languages : en
Pages : 675
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Book Description
Author: J. Robin Harris
Publisher: Springer Science & Business Media
ISBN: 1461303257
Category : Science
Languages : en
Pages : 446
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Book Description
In Volume 25, leading experts present studies on the value of increased ascorbic acid intake and explore its specific contributions to human and animal health.
Author: Hans J. Vogel
Publisher: Springer Science & Business Media
ISBN: 1592591841
Category : Science
Languages : en
Pages : 415
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Book Description
Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on–off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.
Author: Ronald J. Clarke
Publisher: John Wiley & Sons
ISBN: 1119085136
Category : Science
Languages : en
Pages : 491
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Book Description
Describes experimental methods for investigating the function of pumps, channels and transporters Covers new emerging analytical methods used to study ion transport membrane proteins such as single-molecule spectroscopy Details a wide range of electrophysiological techniques and spectroscopic methods used to analyze the function of ion channels, ion pumps and transporters Covers state-of-the art analytical methods to study ion pumps, channels, and transporters, and where analytical chemistry can make further contributions
Author: B.B. Biswas
Publisher: Springer Science & Business Media
ISBN: 1461303435
Category : Science
Languages : en
Pages : 433
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Book Description
`The heterogeneity of topics ... is very ambitious, and the result is, overall, successful because of the high quality of the individual contributions ... highly recommended.' -American Scientist, from a review of a previous volume Volume 26 examines the emerging areas of signal transduction based on myoinositol phosphates and Ca2+ while focusing on plant and animal responses. Chapters explore synthesis, separation, and identification of different inositol phosphates.
Author: Eric Dickinson
Publisher: Royal Society of Chemistry
ISBN: 1847550835
Category : Technology & Engineering
Languages : en
Pages : 428
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Book Description
Food scientists aim to control the taste and texture of existing food products and to formulate new structures of high quality using novel combinations of ingredients and processing methods. Food Colloids, Biopolymers and Materials describes the physical chemistry and material science underlying the formulation and behaviour of multi-phase food systems and includes: * descriptions of new experimental techniques * recent food colloids research findings * authoritative overviews of conceptual issues Essential new findings are presented and emphasis is placed on the interfacial and gelation properties of food proteins, and the role of colloidal and biopolymer interactions in determining the properties of emulsions, dispersions, gels and foams. Specific topics include: confocal microscopy; diffusing wave spectroscopy; protein-polysaccharide interactions; biopolymer phase separation; fat crystallization; bubble/droplet coalescence; and bulk and surface rheology. This book is the latest addition to the highly regarded food colloid series published by the Royal Society of Chemistry and is of relevance to those working and researching in food science and surface and colloid science.
