Physical and Biochemical Factors Affecting the Recovery and Analysis of DNA from Human Skeletal Remains PDF Download
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Author: Laura Gaydosh Combs
Publisher:
ISBN:
Category :
Languages : en
Pages : 140
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Book Description
There are approximately 4,400 sets of unidentified human remains recovered each year, nearly a quarter of which are not identified within the year following recovery. Obtaining genetic information through DNA testing of bone samples has become a critical element to identifying missing persons and recovered human remains. DNA is preserved within the structure of bone for vast amounts of time, surviving environmental and microbial insults, yet bone is one the most challenging sample types encountered by forensic scientists. This is due to the resilient structure of bone and the prevalence and variety of materials which co-isolate with DNA during extraction and function as inhibitors of the polymerase chain reaction (PCR). Bone-associated PCR inhibitors include native components and environmental materials, acquired as a consequence of the porous composition of bone. Quality assurance requirements governing DNA testing laboratories do not mandate direct evaluation of the product of the DNA extraction process; coupled with poor characterization of PCR inhibitors, the forensic community has not adequately demonstrated the efficiency of methods used to extract DNA from bone samples. The primary hypothesis is failure of PCR-based testing of DNA from skeletal remains is frequently encountered due to inefficient extraction methods and PCR inhibition. This dissertation project has: 1) demonstrated an approach for identifying and characterizing putative PCR inhibitors, emphasizing those originating from the mineral contents of bone; and, 2) assessed the efficiency of current methods used for extracting DNA from bone samples, in terms of quality and quantity of the recovered template. Control genomic DNA, bone samples from adjudicated forensic cases obtained from the University of North Texas Center for Human Identification, and cadaver bone samples obtained from the Willed Body Program at University of North Texas Health Science Center were used for experiments. Laboratory experiments included: DNA extraction, analysis of DNA fragmentation, quantification of DNA, amplification of short tandem repeat (STR) forensic loci, genetic analysis, and elemental analyses that were conducted in collaboration with the University of North Texas Department of Chemistry and Forensic Science Program.
Author: Laura Gaydosh Combs
Publisher:
ISBN:
Category :
Languages : en
Pages : 140
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Book Description
There are approximately 4,400 sets of unidentified human remains recovered each year, nearly a quarter of which are not identified within the year following recovery. Obtaining genetic information through DNA testing of bone samples has become a critical element to identifying missing persons and recovered human remains. DNA is preserved within the structure of bone for vast amounts of time, surviving environmental and microbial insults, yet bone is one the most challenging sample types encountered by forensic scientists. This is due to the resilient structure of bone and the prevalence and variety of materials which co-isolate with DNA during extraction and function as inhibitors of the polymerase chain reaction (PCR). Bone-associated PCR inhibitors include native components and environmental materials, acquired as a consequence of the porous composition of bone. Quality assurance requirements governing DNA testing laboratories do not mandate direct evaluation of the product of the DNA extraction process; coupled with poor characterization of PCR inhibitors, the forensic community has not adequately demonstrated the efficiency of methods used to extract DNA from bone samples. The primary hypothesis is failure of PCR-based testing of DNA from skeletal remains is frequently encountered due to inefficient extraction methods and PCR inhibition. This dissertation project has: 1) demonstrated an approach for identifying and characterizing putative PCR inhibitors, emphasizing those originating from the mineral contents of bone; and, 2) assessed the efficiency of current methods used for extracting DNA from bone samples, in terms of quality and quantity of the recovered template. Control genomic DNA, bone samples from adjudicated forensic cases obtained from the University of North Texas Center for Human Identification, and cadaver bone samples obtained from the Willed Body Program at University of North Texas Health Science Center were used for experiments. Laboratory experiments included: DNA extraction, analysis of DNA fragmentation, quantification of DNA, amplification of short tandem repeat (STR) forensic loci, genetic analysis, and elemental analyses that were conducted in collaboration with the University of North Texas Department of Chemistry and Forensic Science Program.
Author: Bradley J. Adams
Publisher: Springer Science & Business Media
ISBN: 1597453161
Category : Medical
Languages : en
Pages : 377
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Book Description
Commingling of human remains presents an added challenge to all phases of the forensic process. This book brings together tools from diverse sources within forensic science to offer a set of comprehensive approaches to handling commingled remains. It details the recovery of commingled remains in the field, the use of triage in the assessment of commingling, various analytical techniques for sorting and determining the number of individuals, the role of DNA in the overall process, ethical considerations, and data management. In addition, the book includes case examples that illustrate techniques found to be successful and those that proved problematic.
Author: Hirak Ranjan Dash
Publisher: Springer
ISBN: 9789811643170
Category : Science
Languages : en
Pages : 1206
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Book Description
This reference book comprehensively reviews the significance of DNA technology in forensic science. After presenting the theory, basic principles, tools and techniques that are used in forensic DNA typing, it summarizes various techniques, including autosomal STR, Y-STR, X-STR, mitochondrial DNA and NGS, used in solving both criminal as and civil cases, such as paternity disputes, identification of mutilated remains, and culprit identification in sexual assault and murder cases. It also provides an overview of DNA-based genetic diagnostics for various diseases, and discusses the role of DNA typing in drug reactions, as well as the application of non-human DNA profiling of animals and plants in forensic science investigations. Lastly, the book examines the role of internal quality control in maintaining the high quality of DNA profiling.
Author: Burkhard Madea
Publisher: CRC Press
ISBN: 1444181777
Category : Law
Languages : en
Pages : 292
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Book Description
Estimation of the Time Since Death remains the foremost authoritative book on scientifically calculating the estimated time of death postmortem. Building on the success of previous editions which covered the early postmortem period, this new edition also covers the later postmortem period including putrefactive changes, entomology, and postmortem r
Author: Aurore Schmitt
Publisher: Springer Science & Business Media
ISBN: 1597450995
Category : Medical
Languages : en
Pages : 462
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Book Description
Recent political, religious, ethnic, and racial conflicts, as well as mass disasters, have significantly helped to bring to light the almost unknown dis- pline of forensic anthropology. This science has become particularly useful to forensic pathologists because it aids in solving various puzzles, such as id- tifying victims and documenting crimes. On topics such as mass disasters and crimes against humanity, teamwork between forensic pathologists and for- sic anthropologists has significantly increased over the few last years. This relationship has also improved the study of routine cases in local medicolegal institutes. When human remains are badly decomposed, partially skelet- ized, and/or burned, it is particularly useful for the forensic pathologist to be assisted by a forensic anthropologist. It is not a one-way situation: when the forensic anthropologist deals with skeletonized bodies that have some kind of soft tissue, the advice of a forensic pathologist would be welcome. Forensic anthropology is a subspecialty/field of physical anthropology. Most of the background on skeletal biology was gathered on the basis of sk- etal remains from past populations. Physical anthropologists then developed an indisputable “know-how”; nevertheless, one must keep in mind that looking for a missing person or checking an assumed identity is quite a different matter. Pieces of information needed by forensic anthropologists require a higher level of reliability and accuracy than those granted in a general archaeological c- text. To achieve a positive identification, findings have to match with e- dence, particularly when genetic identification is not possible.
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309045878
Category : Science
Languages : en
Pages : 199
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Book Description
Matching DNA samples from crime scenes and suspects is rapidly becoming a key source of evidence for use in our justice system. DNA Technology in Forensic Science offers recommendations for resolving crucial questions that are emerging as DNA typing becomes more widespread. The volume addresses key issues: Quality and reliability in DNA typing, including the introduction of new technologies, problems of standardization, and approaches to certification. DNA typing in the courtroom, including issues of population genetics, levels of understanding among judges and juries, and admissibility. Societal issues, such as privacy of DNA data, storage of samples and data, and the rights of defendants to quality testing technology. Combining this original volume with the new update-The Evaluation of Forensic DNA Evidence-provides the complete, up-to-date picture of this highly important and visible topic. This volume offers important guidance to anyone working with this emerging law enforcement tool: policymakers, specialists in criminal law, forensic scientists, geneticists, researchers, faculty, and students.
Author: M. Anne Katzenberg
Publisher: John Wiley & Sons
ISBN: 1119151619
Category : Social Science
Languages : en
Pages : 688
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Book Description
An Indispensable Resource on Advanced Methods of Analysis of Human Skeletal and Dental Remains in Archaeological and Forensic Contexts Now in its third edition, Biological Anthropology of the Human Skeleton has become a key reference for bioarchaeologists, human osteologists, and paleopathologists throughout the world. It builds upon basic skills to provide the foundation for advanced scientific analyses of human skeletal remains in cultural, archaeological, and theoretical contexts. This new edition features updated coverage of topics including histomorphometry, dental morphology, stable isotope methods, and ancient DNA, as well as a number of new chapters on paleopathology. It also covers bioarchaeological ethics, taphonomy and the nature of archaeological assemblages, biomechanical analyses of archaeological human skeletons, and more. Fully updated and revised with new material written by leading researchers in the field Includes many case studies to demonstrate application of methods of analysis Offers valuable information on contexts, methods, applications, promises, and pitfalls Covering the latest advanced methods and techniques for analyzing skeletal and dental remains from archaeological discoveries, Biological Anthropology of the Human Skeleton is a trusted text for advanced undergraduates, graduate students, and professionals in human osteology, bioarchaeology, and paleopathology.
Author: Tim D. White
Publisher: Elsevier
ISBN: 0080488994
Category : Law
Languages : en
Pages : 485
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Book Description
Building on the success of their previous book, White and Folkens' The Human Bone Manual is intended for use outside the laboratory and classroom, by professional forensic scientists, anthropologists and researchers. The compact volume includes all the key information needed for identification purposes, including hundreds of photographs designed to show a maximum amount of anatomical information. - Features more than 500 color photographs and illustrations in a portable format; most in 1:1 ratio - Provides multiple views of every bone in the human body - Includes tips on identifying any human bone or tooth - Incorporates up-to-date references for further study
Author: Beth Alison Shapiro
Publisher:
ISBN: 9781617795169
Category : Nature
Languages : en
Pages : 247
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Book Description
Ancient DNA presents an overview of the many of the protocols commonly used to study ancient DNA. These include laboratory instructions, extraction protocols, laboratory techniques, and suggestions for appropriate analytical approaches to make sense of the sequences obtained.
Author: Sarah Ellingham
Publisher: John Wiley & Sons
ISBN: 1119682746
Category : Medical
Languages : en
Pages : 485
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Book Description
BURNT HUMAN REMAINS An all-encompassing reference and guide designed for professionals involved in the forensic analysis of burnt remains Burnt Human Remains: Recovery, Analysis and Interpretation presents an in-depth multidisciplinary approach to the detection, recovery, analysis, and identification of thermally altered remains. Bridging the gap between research and practice, this invaluable one-stop reference provides detailed coverage of analytical techniques in forensic medicine and pathology, forensic anthropology, forensic odontology, and forensic chemistry and forensic biology. Contributions from a panel of expert authors review the newest findings in forensics research and discuss their applicability to forensic case work. Opening with a historical overview of the discipline, the book covers the search and recovery aspects of burnt human remains, medico-legal investigations, determination of the post mortem interval of burnt remains, structural changes of burnt bone and teeth, DNA extraction from burnt remains, and much more. Throughout the text, the authors emphasize the importance of understanding the changes undergone by bodies when subjected to fire for establishing identity, reconstructing the events leading up to incineration, and determining the cause and manner of death. Provides a systemic framework that integrates established forensic methods and state-of-the-art analytical approaches Describes different forensic analyses from the macroscopical, microscopical, biochemical, and molecular level Features international case studies of challenging individual cases as well as natural or man-made mass fatalities requiring the identification of incinerated remains Demonstrates how changes to the macro- and microstructure of burnt remains can reveal information about incineration conditions Discusses organizations and programs focused on developing standards and best practice for the recovery and analysis burnt remains Burnt Human Remains: Recovery, Analysis and Interpretation is an indispensable resource for all practitioners engaged in the interpretation of burned human tissue, including pathologists, forensic chemists, forensic biologists, forensic anthropologists, forensic odontologists, and archaeologists.
