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Author: Sylvia Daunert
Publisher: John Wiley & Sons
ISBN: 9783527310166
Category : Science
Languages : en
Pages : 264
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Book Description
The use of light-emitting proteins for the detection of biomolecules provides fast and sensitive methods which overcome the disadvantages of radioactive labels and the high cost of fluorescent dyes. This reference work summarizes modern advanced techniques and their applications and includes practical examples of assays based on photoproteins. The book presents contemporary key topics like luminescent marine organisms, DNA probes, reporter gene assays and photoproteins, ratiometric sensing, use of photoproteins for in vivo functional imaging and luminescent proteins in binding assays, to name just a few, and is complemented by recent advances in instrumentation. Includes an introductory chapter by 2008 Chemistry Nobel laureate Osamu Shimomura.
Author: Sylvia Daunert
Publisher: John Wiley & Sons
ISBN: 9783527310166
Category : Science
Languages : en
Pages : 264
Get Book Here
Book Description
The use of light-emitting proteins for the detection of biomolecules provides fast and sensitive methods which overcome the disadvantages of radioactive labels and the high cost of fluorescent dyes. This reference work summarizes modern advanced techniques and their applications and includes practical examples of assays based on photoproteins. The book presents contemporary key topics like luminescent marine organisms, DNA probes, reporter gene assays and photoproteins, ratiometric sensing, use of photoproteins for in vivo functional imaging and luminescent proteins in binding assays, to name just a few, and is complemented by recent advances in instrumentation. Includes an introductory chapter by 2008 Chemistry Nobel laureate Osamu Shimomura.
Author: Axel Griesbeck
Publisher: CRC Press
ISBN: 1466561254
Category : Medical
Languages : en
Pages : 1607
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Book Description
The only combined organic photochemistry and photobiology handbookAs spectroscopic, synthetic and biological tools become more and more sophisticated, photochemistry and photobiology are merging-making interdisciplinary research essential. Following in the footsteps of its bestselling predecessors, the CRC Handbook of Organic Photochemistry and Pho
Author: Gérald Thouand
Publisher: Springer
ISBN: 3319274074
Category : Science
Languages : en
Pages : 202
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Book Description
This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English. /div
Author: Maurice Goeldner
Publisher: John Wiley & Sons
ISBN: 3527605541
Category : Science
Languages : en
Pages : 584
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Book Description
With contributions by more than 30 expert researchers, this handbook covers the whole spectrum from chemistry to cell biology and from theory to application. In so doing, it deals with a broad range of topics from the chemistry and biophysics of caged compounds to their application in time-resolved studies, comparing the properties of different caging groups. The authors describe in detail light-activation of proteins as well as nucleic acids, while a special section is devoted to multiphoton phototriggers. A must-have for every biochemist, biophysicist and molecular biologist developing and working with these novel methods.
Author: Shimshon Belkin
Publisher: Springer
ISBN: 3642123627
Category : Science
Languages : en
Pages : 208
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Book Description
Table of Contents Reporter cells: - Yeast Based Sensors by Mifumi Shimomura-Shimizu and Isao Karube - Mammalian Cell-Based Sensor Systems by Pratik Banerjee, Briana Franz, and Arun K. Bhunia - Fluorescent and Bioluminescent Cell-Based Sensors: Strategies for Their Preservation by Amol Date, Patrizia Pasini, and Sylvia Daunert - Electrochemical Cell-Based Sensors by Eliora Z. Ron and Judith Rishpon - Microbial Cell Arrays by Tal Elad, Jin Hyung Lee, Man Bock Gu, and Shimshon Belkin Devices: - Surface Functionalization for Protein and Cell Patterning by Pascal Colpo , Ana Ruiz , Laura Ceriotti , and François Rossi - Fiber-Optic Based Cell Sensors by Evgeni Eltzov and Robert S. Marks - Electronic Interfacing with Living Cells by James T. Fleming - On-Chip Detection of Cellular Activity by R. Almog , R. Daniel , S. Vernick , A. Ron , H. Ben-Yoav, and Y. Shacham-Diamand
Author: Hirobumi Suzuki
Publisher: BoD – Books on Demand
ISBN: 1839623853
Category : Technology & Engineering
Languages : en
Pages : 136
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Book Description
This book contains seven chapters on bioluminescence techniques and organisms. On the technical side, the four chapters presented the fluorescent markers of proteins and nanocrystals, imidazopyrazine-type luciferin that emits light when bound to human serum albumin, firefly luciferin that emits near-infrared light, and imaging technique for visualization of promoter activity in fruiting body formation of cellular slime molds Dictyostelium. On the organismal side, the three chapters presented recommendations for the commercial use of fireflies in urban areas from the perspective of conservation biology, the origin of luciferin by predation in marine luminescent organisms, and the ecology and behavior of luminescent organisms from sea to land, which will be of interest to both professionals and students.
Author: Hugo Barrera-Saldaña
Publisher: BoD – Books on Demand
ISBN: 9533077905
Category : Science
Languages : en
Pages : 272
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Book Description
Leading scientists from different countries around the world contributed valuable essays on the basic applications and safety, as well as the ethical and moral considerations, of the powerful genetic engineering tools now available for modifying the molecules, pathways, and phenotypes of species of agricultural, industrial and even medical importance. After three decades of perfecting such tools, we now see a refined technology, surprisingly unexpected applications, and matured guidelines to avoid unintentional damage to our and other species, as well as the environment, while trying to contribute to solve the biological, medical and technical challenges of society and industry. Chapters on thermo-stabilization of luciferase, engineering of the phenylpropanoid pathway in a species of high demand for the paper industry, more efficient regeneration of transgenic soybean, viral resistant plants, and a novel approach for rapidly screening properties of newly discovered animal growth hormones, illustrate the state-of-the-art science and technology of genetic engineering, but also serve to raise public awareness of the pros and cons that this young scientific discipline has to offer to mankind.
Author: Lawrence W. Miller
Publisher: John Wiley & Sons
ISBN: 9783527315666
Category : Science
Languages : en
Pages : 204
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Book Description
The only resource to systematically review current experimental methods, this handy reference enables researchers to select the best solution for their experimental problems. For each method covered, the book provides step-by-step protocols, illustrated by typical research applications. After an initial section on probing the lipid bilayer, the text moves on to discuss probing proteins -- including membrane proteins -- and nucleic acids. The first single publication to incorporate chemical markers, fluorescent probes and genetic tags allows a well-informed comparison of different solutions for the same problem in molecular analytics.
Author: Jeffrey J. Clare
Publisher: John Wiley & Sons
ISBN: 3527607935
Category : Science
Languages : en
Pages : 302
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Book Description
Filling the gap created over the past five years, during which many new techniques have entered the market, this book is directed at both the new and the experienced ion channel researcher wishing to learn more about the considerations and methods for studying recombinant ion channels. These latest developments are covered here for the first time, contributed by editors and authors working for major pharmaceutical companies and who routinely apply these techniques in their daily work. The first three chapters cover the use of the Xenopus oocyte expression system for structure-function studies, from basic approaches for manipulating ion channel cDNAs to more specialized but powerful techniques. This is followed by reviews of strategies and methodologies available for expressing channels in mammalian cells and for their analysis by patch-clamp electrophysiology. Chapters 6 to 8 review the latest methodologies for ion channel drug discovery, including high throughput screening using fluorescence and luminescence, as well as automated planar array electrophysiology. The remaining two chapters focus on approaches for determining ion channel crystal structures and on computational approaches to understanding channel mechanisms at atomic resolution. Rather than provide detailed protocols, indicated by references in each chapter, the authors provide a comprehensive and easily accessible overview of the techniques involved, reviewing underlying principles and providing working guidelines as well as an understanding of the key theoretical and practical considerations associated with each topic. In each case, this practical advice is illustrated by real life examples, taken either from the author's own experience or from key examples in the literature, providing valuable practical hints not found elsewhere. The result is a compendium of practical ion channel information that will prove a valuable resource to academic and industrial workers alike.
Author: Aldo Roda
Publisher: Royal Society of Chemistry
ISBN: 1847558127
Category : Medical
Languages : en
Pages : 609
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Book Description
This comprehensive overview of chemiluminescence and bioluminescence covers historical developments, fundamental principles, recent advances, instrumentation, and applications.
