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Author:
Publisher: Academic Press
ISBN: 0128170298
Category : Science
Languages : en
Pages : 300
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Book Description
Optochemical Biology, Volume 624, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters on a variety of topics, including Cell Lineage Tracing in Zebrafish Embryos with an Expanded Genetic Code, Optical Control of Tumor Induction in the Zebrafish, Optogenetic Control by Pulsed Illumination, Optimizing the Photocontrol of bZIP Coiled Coils with Azobenzene Crosslinkers: Role of the Crosslinking Site, Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein, In Situ Formation of an Azo Bridge on Proteins Controllable by Visible Light, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Includes the latest information on optochemical biology
Author:
Publisher: Academic Press
ISBN: 0128170298
Category : Science
Languages : en
Pages : 300
Get Book Here
Book Description
Optochemical Biology, Volume 624, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters on a variety of topics, including Cell Lineage Tracing in Zebrafish Embryos with an Expanded Genetic Code, Optical Control of Tumor Induction in the Zebrafish, Optogenetic Control by Pulsed Illumination, Optimizing the Photocontrol of bZIP Coiled Coils with Azobenzene Crosslinkers: Role of the Crosslinking Site, Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein, In Situ Formation of an Azo Bridge on Proteins Controllable by Visible Light, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Includes the latest information on optochemical biology
Author: Andrea Cusano
Publisher: Taylor & Francis
ISBN: 1439854904
Category : Science
Languages : en
Pages : 613
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Book Description
This book provides an overview of the state of the art in optical and chemical nanosensors for industrial, environmental, diagnostic, security, and medical applications. It summarizes the various types and developments in optical and chemical sensor technology and then explains how the integration of optical/chemical sensors and nanomaterials creates new opportunities. The text also reviews optochemical sensors, starting from the basics in optoelectronics and concluding with the principles of operation at the basis of optochemical devices. The authors offer insight into future trends in this growing field and present a range of applications in the fields of medicine, security, and bioterrorism.
Author: Ehud Y. Isacoff
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
The transmembrane proteins that underlie neural processing are now known at a level of detail that has greatly increased our understanding of these sophisticated molecular machines. Starting with MacKinnon's seminal structure of a potassium channel, several voltage-gated ion channels and ionotropic receptors have been revealed with atomic resolution (Figure 3.1) [2, 3, 4, 5, 6]. This has been complemented by structures of G-protein coupled receptors, adding opsins and metabotropic receptors to the ever-increasing repertoire of transmembrane proteins elucidated with structural biology [7, 8, 9, 10]. As a consequence of this structural revolution and recent advances in pharmacology, Nature's molecular machines can now be manipulated with relative ease. This can be done, for instance, via synthetic on-off switches or tuning elements that are attached to the signaling protein of interest to allow for its orthogonal control with non-natural input signals. Amongst these signals, light is particularly useful, since it is unmatched in terms of temporal and spatial precision and techniques for the delivery and control of light are highly developed.
Author: Chanat Aonbangkhen
Publisher:
ISBN:
Category :
Languages : en
Pages : 516
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Book Description
Fundamental biological processes including cell division, migration, and death, are driven by protein interactions. Regulation of protein localization is one of the mechanisms cells utilize to control cellular events with high spatial and temporal precision. Therefore, several techniques have been developed to provide control of protein interactions and localization. A number of elegant approaches employ naturally light-responsive proteins, also known as optogenetics, to reversibly induce protein? protein binding interactions with subcellular precision. However, the application of these light-inducible protein systems to various intracellular locations beyond the plasma membrane has been limited. Moreover, to achieve sustained interactions in some applications, most of these optogenetic systems require continuous illumination, increasing the risk of phototoxicity. Another robust and widely utilized technique to control protein interactions via small molecules is the chemically-induced dimerization (CID) of proteins; the most classic example of this technique being rapamycin-induced dimerization. However, the lack of spatiotemporal control and reversibility in this system has necessitated the development of new dimerizers in the past two decades. By combining light-inducible features with the CID technique, we have created a novel platform to rapidly and reversibly induce protein dimerization using light with high specificity in living cells. This is accomplished with subcellular spatiotemporal resolution using a series of novel, cell-permeable, photoactivatable, and photocleavable chemical dimerizers. The modular design of our system has allowed us to tailor the properties of our molecules for studying various protein functions and biological pathways inside living cells. Furthermore, we demonstrate the utility of our system by applying it to manipulate dynamic biological events including organelle transport and spindle assembly checkpoint. This work establishes a foundation for optogenetic control over protein function and highlights the advantages of a hybrid chemical and genetic approach. We envision our tools to be readily adapted to experimentally probe complex signaling networks and other cellular processes that depend upon spatiotemporal regulation of protein localization on biologically-relevant timescales.
Author: Christian Dahmann
Publisher: Springer Nature
ISBN: 1071625411
Category : Science
Languages : en
Pages : 423
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Book Description
This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and cell lineage tracing, protein inhibition and tagging, optogenetic and optochemical control of tissue mechanics, AFM measurements, sample preparation for electron microscopy, live imaging of different tissues and organs, and quantitative image analysis. Written in the format of the highly successful Methods in Molecular Biology series, chapters include an introduction to their topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Drosophila: Methods and Protocols, Third Edition serves as a useful and practical guide to new researchers and experts using Drosophila as a model system. Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Author: Yu Sun
Publisher: Springer Nature
ISBN: 3030801977
Category : Technology & Engineering
Languages : en
Pages : 422
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Book Description
This book describes the substantial progress recently made in the development of micro and nanorobotic systems, utilizing magnetic, optical, acoustic, electrical, and other actuation fields. It covers several areas of micro and nanorobotics including robotics, materials science, and biomedical engineering. Field-Driven Micro and Nanorobots for Biology and Medicine provides readers with fundamental physics at the micro and nano scales, state-of-the-art technical advances in field-driven micro and nanorobots, and applications in biological and biomedical disciplines.
Author:
Publisher: Academic Press
ISBN: 0128201487
Category : Science
Languages : en
Pages : 360
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Book Description
Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems, Volume 638, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sample chapters from this new release include In vitro characterization of the colibactin-activating peptidase ClbP enables development of a fluorogenic activity probe, Using FDAA probes to study cell division in Bacillus subtilis, Chemoenzymatic synthesis of UDP-sugars, Chemical tools for selective activity profiling of bacterial penicillin-binding proteins, Chemical Probes Reveal and Extraseptal Mode of Cross-linking in Staphylococcus Aureus, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on retinoid signaling pathways
Author: Howard C. Hang
Publisher: John Wiley & Sons
ISBN: 352734733X
Category : Science
Languages : en
Pages : 806
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Book Description
Advanced Chemical Biology The modern approach to teaching chemical biology Advanced Chemical Biology is organized around the central dogma of life, progressing from genes to proteins and higher-order cellular structures, including core application areas such as imaging, chemical genetics, activity-based protein profiling, and natural product discovery and biosynthesis. Advanced topics and applications in, e. g., microbiology, developmental biology, and neurobiology, are covered in separate sections. Every chapter is homogeneous in style and layout, consisting of a short historical introduction followed by a description of the underlying concepts and a selection of recent examples of how the concept has been turned into practice. The subdivision of the contents into core and supplemental chapters enables a flexible use in teaching, both for a one-semester and a two-semester course. Written by authors and editors coming from the leading scientific institutions that have developed the concepts and technologies for this discipline, Advanced Chemical Biology includes specific information on topics like: DNA function, synthesis and engineering, chemical approaches to genome integrity, and RNA function, synthesis, and probing Chemical approaches to transcription and RNA regulation in vivo, chemical biology of genome engineering, and peptide/protein synthesis and engineering Directed evolution for chemical biology, chemical biology of cellular metabolism, chemical biology of lipids, and protein post-translational modifications Chemical glycobiology, chemical and enzymatic modification of proteins, genetic code expansion, bio-orthogonal chemistry, and cellular imaging With its broad scope and focus on turning concepts into applications, Advanced Chemical Biology is an excellent starting point for anyone entering the field and looking for a guide to the wide range of available methods and strategies that chemical biology has to offer. With a Foreword by Nobel Laureate Carolyn Bertozzi.
Author: Joachim Hesse
Publisher: John Wiley & Sons
ISBN: 3527620737
Category : Science
Languages : en
Pages : 734
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Book Description
'Sensors' is the first self-contained series to deal with the whole area of sensors. It describes general aspects, technical and physical fundamentals, construction, function, applications and developments of the various types of sensors. This is the first of two volumes focusing on chemical and biochemical sensors providing definitions, typical examples of chemical and biochemical sensors and historical remarks. It describes chemical sensor technologies and interdisciplinary tasks in the design of chemical sensors. The major part consists of a description of basic sensors. They include electrolyte sensors, solid electrolyte sensors, electronic conductivity and capacitance sensors, field effect sensors, calorimetric sensors, optochemical sensors, and mass sensitive sensors. This volume is an indispensable reference work for both specialists and newcomers, researchers and developers.
Author: Dominik Niopek
Publisher: Humana
ISBN: 9781071607572
Category : Medical
Languages : en
Pages : 287
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Book Description
This volume provides cutting edge protocols for using optogenetic and opto-chemical techniques across a wide spectrum of applications. Chapters guide readers through methods in neuro-optogenetics, including methods to dissect local and long-range connectivity ex vivo, investigate G-protein coupled receptors, study astrocyte-neuron networks in vitro and in vivo, employ upconversion nanoparticles for transcranial optogenetic stimulation, and use Adeno-associated virus (AAV) for delivery of optogenetic constructs. At the same time, this book covers a range of methods for non-neuronal optogenetics, including various applications of light-oxygen-voltage domains or cryptochromes for control of nucleocytoplasmic transport, transgene expression and chromatin recruitment. The book further presents protocols for traceless light-control of gene expression with melanopsin, light-inducible cAMP production, use of light-regulated receptor tyrosine kinases (Opto-RTKs), synthesis of light-dependent biomaterials, and highlights emerging tools at the intersection of optogenetics and CRISPR. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Photoswitching Proteins: Methods and Protocols aims to provide a comprehensive list of methods and tools to investigate highly dynamic pathways and behaviors in cells and animals using optogenetics.
