On the Capillary Electrophoresis of Monohydroxy Metabolites of Polycyclic Aromatic Hydrocarbons and Its Application to the Analysis of Biological Matrices PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download On the Capillary Electrophoresis of Monohydroxy Metabolites of Polycyclic Aromatic Hydrocarbons and Its Application to the Analysis of Biological Matrices PDF full book. Access full book title On the Capillary Electrophoresis of Monohydroxy Metabolites of Polycyclic Aromatic Hydrocarbons and Its Application to the Analysis of Biological Matrices by Gaston Knobel. Download full books in PDF and EPUB format.
Author: Gaston Knobel
Publisher:
ISBN:
Category :
Languages : en
Pages : 137
Get Book Here
Book Description
Chromatographic separation and quantification has been based on high-performance liquid chromatography-room temperature fluorescence detection (HPLC) and gas chromatography-mass spectrometry (GC-MS). Although chromatographic techniques provide reliable results in the analysis of OH-PAH, their experimental procedures are time consuming and expensive. Elution times of 30-60 minutes are typical and standards must be run periodically to verify retention times. If the concentrations of target species are found to lie outside the detector's response range, the sample must be diluted and the process repeated. On the other end of the concentration range, many samples are "zeroes," i.e. the concentrations are below detection limits. Additional problems arise when laboratory procedures are scaled up to handle thousands of samples under mass screening conditions. Under the prospective of a sustainable environment, the large usage of organic solvents is one of the main limitations of the current chromatographic methodology.
Author: Gaston Knobel
Publisher:
ISBN:
Category :
Languages : en
Pages : 137
Get Book Here
Book Description
Chromatographic separation and quantification has been based on high-performance liquid chromatography-room temperature fluorescence detection (HPLC) and gas chromatography-mass spectrometry (GC-MS). Although chromatographic techniques provide reliable results in the analysis of OH-PAH, their experimental procedures are time consuming and expensive. Elution times of 30-60 minutes are typical and standards must be run periodically to verify retention times. If the concentrations of target species are found to lie outside the detector's response range, the sample must be diluted and the process repeated. On the other end of the concentration range, many samples are "zeroes," i.e. the concentrations are below detection limits. Additional problems arise when laboratory procedures are scaled up to handle thousands of samples under mass screening conditions. Under the prospective of a sustainable environment, the large usage of organic solvents is one of the main limitations of the current chromatographic methodology.
Author: Rawi Ramautar
Publisher: John Wiley & Sons
ISBN: 3527833102
Category : Science
Languages : en
Pages : 404
Get Book Here
Book Description
Capillary Electrophoresis—Mass Spectrometry for Proteomics and Metabolomics A powerful and essential resource for researchers with an interest in CE-MS In Capillary Electrophoresis—Mass Spectrometry for Proteomics and Metabolomics: Principles and Applications, a team of distinguished researchers delivers a comprehensive overview of bioanalytical capillary electrophoresis coupled to mass spectrometry (CE-MS). The book explains foundational principles, technology as well the strategies and techniques used in data analysis for metabolic and proteomic studies. It also provides a global overview of recent developments and advances for improving CE-MS sensitivity and reproducibility. An essential handbook for everyone performing metabolomic and proteomic analysis, the information provided here will assist researchers in tapping into the full potential of this technique to answer biological and clinical questions. Readers will also find: A thorough introduction to the principles of capillary electrophoresis, including its fundamentals, CE separation modes, capillary coatings, and the fundamentals of mass spectrometry In-depth examinations of technological developments in capillary electrophoresis, including sample preparation, online preconcentration, detection sensitivity, and metabolic coverage Comprehensive discussions of metabolomic studies, including their biomedical and clinical applications Recent advances in proteomics, including top-down and bottom-up approaches Perfect for analytical and clinical chemists, Capillary Electrophoresis—Mass Spectrometry for Proteomics and Metabolomics: Principles and Applications will also earn a place in the libraries of biochemists, molecular biologists, and other molecular life scientists.
Author: Rawi Ramautar
Publisher: Royal Society of Chemistry
ISBN: 1788014847
Category : Science
Languages : en
Pages : 300
Get Book Here
Book Description
Capillary electrophoresis–mass spectrometry (CE-MS) has become a very useful analytical technique for the profiling of highly polar and charged metabolites in biological samples. In this book, the unique features of CE-MS for metabolomics studies are highlighted including CE separation modes, capillary coatings and practical aspects of CE-MS coupling alongside a comprehensive overview of recent technological developments and applications. CE-MS can be considered a relatively new technique in the field of metabolomics and it is therefore important to inform the scientific community about the possibilities of advanced CE-MS approaches for metabolomics studies. This book outlines the potential of this technique for researchers working in metabolomics, bioanalytics and biomarker analysis.
Author: Peter Mikuš
Publisher: BoD – Books on Demand
ISBN: 9535106570
Category : Medical
Languages : en
Pages : 211
Get Book Here
Book Description
The scientific monograph by the author Peter Mikus entitled "Chiral Capillary Electrophoresis in Current Pharmaceutical and Biomedical Analysis" provides a comprehensive view on the advanced capillary electrophoresis techniques aimed to current chiral bioanalysis. The advances in the chiral electrophoresis analytical approaches are divided and theoretically described in three sections involving (i) advanced chiral separations for the optimization of chiral resolution (separation mechanisms; electrophoresis techniques in capillary and microchip format; electrophoretic modes such as ITP, CZE/EKC, CEC; chiral additives / pseudophases / phases), (ii) advanced sample preparation for the on-line preconcentration, sample clean-up and analyte derivatization (implementation of electrophoretic effects such as stacking; non-electrophoretic effects such as SPE, chromatography, dialysis; combinations of these effects; multidimensional CE systems; instrumental schemes), (iii) advanced combinations of detection and electrophoresis for the optimization in qualitative and quantitative evaluation (the most important universal as well as selective detection approaches such as absorption and fluorescence spectrophotometry, electrochemical detection, mass spectrometry vs. (i) and/or (ii)). Real analytical potential (benefits and limitations) of these advanced analytical approaches is emphasized by selected performance parameters of the methods and illustrated by many current practical applications including chiral analyses of drugs, their (bio)degradation products and biomarkers in pharmaceutical and biological matrices. The author wishes the readers many inspirations in the creation of new innovative approaches in the field of advanced chiral electrophoresis techniques with the aim to overcome capabilities of the current analytical techniques.
Author: Aran Paulus
Publisher: Springer Science & Business Media
ISBN: 332285020X
Category : Technology & Engineering
Languages : en
Pages : 192
Get Book Here
Book Description
Wir sind umgeben von Kohlenhydraten: der süße Kaffee, Tee oder Dessert, die Stärke als Hauptkomponente unserer Nahrung und die Zellulose als Strukturelement in Pflanzen. Kohlenhydrate sind eine wichtige Klasse biologischer Moleküle, die an einer Anzahl wichtiger biochemischer Prozesse beteiligt sind. Gerade beginnen wir die Rolle von komplexen Zuckern zu verstehen, die an Proteine gebunden die Kommunikation von Zellen in einer "Zuckersprache" bewerkstelligen. Und nicht zuletzt kommen die ersten Kohlenhydratmoleküle als Medikamente auf den Markt. Anders als für andere Biopolymere sind die analytischen Methoden zur strukturellen Charakterisierung und Sequenzanalyse für Kohlenhydrate zur Zeit ungenügend, zum Teil wegen der überwältigenden Isotopenzahl der Zucker. Dieses Buch beschreibt die Entwicklung der letzten Jahre, die mit der Kapillarelektrophorese in Bezug auf eine miniaturisierte Analytik mit besserer Auflösung und Empfindlichkeit gemacht wurden. Instrumentierung, Derivatisierung, Trennbedingungen und Anwendungen in verschiedenen Disziplinen und Industrien wie z. B. Glykobiologie, Lebensmittelindustrie und Biotechnologie werden beschrieben.
Author: James Q. Xia
Publisher: Springer
ISBN: 9783319462387
Category : Science
Languages : en
Pages : 0
Get Book Here
Book Description
This book covers the latest developments in capillary electrophoresis-mass spectrometry for the analysis of therapeutic proteins. The application of capillary electrophoresis-mass spectrometry (CE-MS) coupling technology in the analysis of recombinant therapeutic proteins is detailed thoroughly. Specific topics include recent developments in coupling capillary electrophoresis with mass spectrometry for the quality control of monoclonal antibody therapeutics, top-down analysis of monoclonal antibody using the CE-MS platform, and detection of host cell protein impurities. Comprehensive characterization of antibody-drug conjugates (ADCs) by coupling capillary electrophoresis with mass spectrometry is also covered. This is an ideal book for scientists in the life science and biopharmaceutical industry who are working on characterizing the PTMs of monoclonal antibodies, as well as graduate students and researchers in the separation science and biological mass spectrometry fields.
Author: Janusz Pawliszyn
Publisher: Elsevier
ISBN: 0123914493
Category : Science
Languages : en
Pages : 497
Get Book Here
Book Description
The relatively new technique of solid phase microextraction (SPME) is an important tool to prepare samples both in the lab and on-site. SPME is a "green" technology because it eliminates organic solvents from analytical laboratory and can be used in environmental, food and fragrance, and forensic and drug analysis. This handbook offers a thorough background of the theory and practical implementation of SPME. SPME protocols are presented outlining each stage of the method and providing useful tips and potential pitfalls. In addition, devices and fiber coatings, automated SPME systems, SPME method development, and In Vivo applications are discussed. This handbook is essential for its discussion of the latest SPME developments as well as its in depth information on the history, theory, and practical application of the method. - Practical application of Solid Phase Microextraction methods including detailed steps - Provides history of extraction methods to better understand the process - Suitable for all levels, from beginning student to experienced practitioner
Author: Raffi Fass
Publisher: Springer Science & Business Media
ISBN: 1461547490
Category : Science
Languages : en
Pages : 353
Get Book Here
Book Description
Proceedings of the 42nd OHOLO Conference held in Eilat, Israel, May 3-7, 1998
Author: Amitava Dasgupta
Publisher: Springer Science & Business Media
ISBN: 1588297802
Category : Medical
Languages : en
Pages : 441
Get Book Here
Book Description
Written in a handbook style with specific methods and tips on eliminating false positive and false negative results, this book is a practical guide to the detailed mechanisms of such occurrences.
Author: Robert J. Hurtubise
Publisher: Wiley-VCH
ISBN:
Category : Phosphorimetry
Languages : en
Pages : 394
Get Book Here
Book Description
