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Author: J. Kevin Foskett
Publisher: New York ; Toronto : Wiley-Liss
ISBN:
Category : Science
Languages : en
Pages : 450
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Book Description
An intriguing and informative guide for cell biologists, cytopathologists, physiologists, cellular biochemists and molecular biologists, this volume provides an up-to-date, representative sample of the latest developments in the area of noninvasive measurements of biological processes at the cellular level. Reviews the latest approaches and techniques for probing a variety of dynamic cellular processes with a minimum of structural or chemical invasion. Topics discussed include: design of novel optical probes, emerging technologies for imaging subcellular processes with high temporal and spatial resolution, the application of noninvasive optical techniques to manipulate cells and subcellular organelles within intact cells.
Author: J. Kevin Foskett
Publisher: New York ; Toronto : Wiley-Liss
ISBN:
Category : Science
Languages : en
Pages : 450
Get Book Here
Book Description
An intriguing and informative guide for cell biologists, cytopathologists, physiologists, cellular biochemists and molecular biologists, this volume provides an up-to-date, representative sample of the latest developments in the area of noninvasive measurements of biological processes at the cellular level. Reviews the latest approaches and techniques for probing a variety of dynamic cellular processes with a minimum of structural or chemical invasion. Topics discussed include: design of novel optical probes, emerging technologies for imaging subcellular processes with high temporal and spatial resolution, the application of noninvasive optical techniques to manipulate cells and subcellular organelles within intact cells.
Author:
Publisher:
ISBN: 9780845133002
Category : Cytology
Languages : en
Pages : 0
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Book Description
Author: S. E. Freeman
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Institute of Medicine
Publisher: National Academies Press
ISBN: 0309224187
Category : Science
Languages : en
Pages : 354
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Book Description
Technologies collectively called omics enable simultaneous measurement of an enormous number of biomolecules; for example, genomics investigates thousands of DNA sequences, and proteomics examines large numbers of proteins. Scientists are using these technologies to develop innovative tests to detect disease and to predict a patient's likelihood of responding to specific drugs. Following a recent case involving premature use of omics-based tests in cancer clinical trials at Duke University, the NCI requested that the IOM establish a committee to recommend ways to strengthen omics-based test development and evaluation. This report identifies best practices to enhance development, evaluation, and translation of omics-based tests while simultaneously reinforcing steps to ensure that these tests are appropriately assessed for scientific validity before they are used to guide patient treatment in clinical trials.
Author: Bruce Alberts
Publisher:
ISBN: 9780815332183
Category : Cytology
Languages : en
Pages : 0
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Book Description
Author: Ammasi Periasamy
Publisher: Springer
ISBN: 1461475139
Category : Science
Languages : en
Pages : 473
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Book Description
Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The authors describe the approaches to selecting epifluorescence microscopy, the detectors, and the image acquisition and processing software for different biological applications. Step-by-step directions on preparing different digital formats for light microscopy images on websites are also provided.
Author: Gerhard Isenberg
Publisher: Springer Science & Business Media
ISBN: 3642803709
Category : Science
Languages : en
Pages : 253
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Book Description
In spite of tremendous scientific progress over the past years, cell biologists do not yet understand the fundamental processes that determine the life cy cle of a cell. Such are: cell movement and cell spreading, cell division, cell communication, cell signaling, cell regeneration and cell death. Biochemistry has enabled us to recognize and to isolate an overwhelming number of new proteins. In vitro assays and the reinjection of proteins into cells and tissues have provided insights into molecular functions and cellular mechanisms. The renaissance of the genetic approach by applying restriction enzymes and vectors, PCR and antisense technology has enabled us to overexpress certain cellular products, to make altered constructs of cell components or to create "knock-out" mutants that entirely lack the factor of interest. Amazingly en ough, all these molecular toys have led to a stream of information but not, in a comparable degree, to a better understanding. Has the puzzle become too complex to get solved; or are the windows too small that we are looking through? As an attempt to answer both questions, the aim of the present mono graph Modern Optics, Electronics and High Precision Techniques in Cell Biol ogy is first to provide cell and molecular biologists with a whole new scope of easily applicable techniques including brand-new optical, biophysical, physicochemical and biosensoric devices. Secondly, these newly developed techniques allow us to look at cells and biological systems as a whole.
Author: Robert D. Goldman
Publisher: CSHL Press
ISBN: 9780879696832
Category : Science
Languages : en
Pages : 658
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Book Description
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
Author:
Publisher: Academic Press
ISBN: 0123918693
Category : Science
Languages : en
Pages : 573
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Book Description
This volume of Methods in Enzymology is the first of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. Expert authors who are leaders in the field Extensively referenced and useful figures and tables Provides hints and tricks to facilitate reproduction of methods
Author:
Publisher: Academic Press
ISBN: 0080859534
Category : Science
Languages : en
Pages : 241
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Book Description
Volume 55 in Methods in Cell Biology is a concise laboratory book that emphasizes the methods and technologies needed to use single polarized laser light source that functions simultaneously as an optical trap and a dual-beam interferometer. * * Provides a practical laboratory guide for methods and technologies used with laser tweezers* Includes comprehensive and easy-to-follow protocols
