Author: Patricia A. Fagan
Publisher:
ISBN:
Category :
Languages : en
Pages : 456
Book Description
NMR Studies of DNA Oligomers and Their Interactions with Minor Groove Binding Ligands
Author: Patricia A. Fagan
Publisher:
ISBN:
Category :
Languages : en
Pages : 456
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 456
Book Description
NMR Studies of DNA Oligomers and Their Interactions with Minor Groove Binding Ligands
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 225
Book Description
The cationic peptide ligands distamycin and netropsin bind noncovalently to the minor groove of DNA. The binding site, orientation, stoichiometry, and qualitative affinity of distamycin binding to several short DNA oligomers were investigated by NMR spectroscopy. The oligomers studied contain A, T-rich or I, C-rich binding sites, where I = 2-desaminodeoxyguanosine. I·C base pairs are functional analogs of A·T base pairs in the minor groove. The different behaviors exhibited by distamycin and netropsin binding to various DNA sequences suggested that these ligands are sensitive probes of DNA structure. For sites of five or more base pairs, distamycin can form 1:1 or 2:1 ligand:DNA complexes. Cooperativity in distamycin binding is low in sites such as AAAAA which has narrow minor grooves, and is higher in sites with wider minor grooves such as ATATAT. The distamycin binding and base pair opening lifetimes of I, C-containing DNA oligomers suggest that the I, C minor groove is structurally different from the A, T minor groove. Molecules which direct chemistry to a specific DNA sequence could be used as antiviral compounds, diagnostic probes, or molecular biology tools. The author studied two ligands in which reactive groups were tethered to a distamycin to increase the sequence specificity of the reactive agent.
Publisher:
ISBN:
Category :
Languages : en
Pages : 225
Book Description
The cationic peptide ligands distamycin and netropsin bind noncovalently to the minor groove of DNA. The binding site, orientation, stoichiometry, and qualitative affinity of distamycin binding to several short DNA oligomers were investigated by NMR spectroscopy. The oligomers studied contain A, T-rich or I, C-rich binding sites, where I = 2-desaminodeoxyguanosine. I·C base pairs are functional analogs of A·T base pairs in the minor groove. The different behaviors exhibited by distamycin and netropsin binding to various DNA sequences suggested that these ligands are sensitive probes of DNA structure. For sites of five or more base pairs, distamycin can form 1:1 or 2:1 ligand:DNA complexes. Cooperativity in distamycin binding is low in sites such as AAAAA which has narrow minor grooves, and is higher in sites with wider minor grooves such as ATATAT. The distamycin binding and base pair opening lifetimes of I, C-containing DNA oligomers suggest that the I, C minor groove is structurally different from the A, T minor groove. Molecules which direct chemistry to a specific DNA sequence could be used as antiviral compounds, diagnostic probes, or molecular biology tools. The author studied two ligands in which reactive groups were tethered to a distamycin to increase the sequence specificity of the reactive agent.
NMR Characterizations of Sequence-specific, Minor-groove-binding, Polyamide Ligands in Complex with DNA Oligomers
Author: Cheryl Augusta Hawkins
Publisher:
ISBN:
Category :
Languages : en
Pages : 318
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 318
Book Description
NMR Studies of Peptides, Distamycin and Its Analogs Bound to the Minor Groove of DNA
Author: Bernhard Hubert Geierstanger
Publisher:
ISBN:
Category :
Languages : en
Pages : 564
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 564
Book Description
NMR Studies of Intermolecular Interactions of Nucleic Acids with Polyamide Ligands and a Synthetic Peptide
Author: Cynthia Ching Chang
Publisher:
ISBN:
Category :
Languages : en
Pages : 392
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 392
Book Description
DNA-Ligand Interactions
Author: W. Guschlbauer
Publisher: Springer Science & Business Media
ISBN: 1468453831
Category : Science
Languages : en
Pages : 289
Book Description
This volume contains the texts of the nineteen lectures presented at the NATO-ASI - FEBS Course on "DNA - ligand interactions: from drugs to proteins." The Advanced Study Institute (ASIl was held from August 30th to September 11th. 1986 in the Abbey of Fontevraud (France). The ASI was attended by 112 participants from a wide scientific horizon and from twentyone different countries. It was in some way a follow-up of the ASI held in Maratea. Italy in May 1981 and which was published in the NATO ASI Life Science series as volume 45. While much has been learned about the way the cellular machinery maintains and transmits the genetic heritage. as well as how these processes are regulated. little is Known about how the interactions between the various partners involved are taKing place. The interactions of drugs and proteins with nucleic acids are of evident importance in the understanding of these problems. The spectacular advances in recombinant DNA technology and the increased sophistication of biophysical techniques. in particular >:-ray diffraction and nuclear magnetic resonance. have created a scientific environment which is highly promising for the future of research in molecular biology. These advances permH the serious hope that biology on the molecular level may become a r-eality. Some of the contributions at the ASI presented the most recent advances in this e>:citing field.
Publisher: Springer Science & Business Media
ISBN: 1468453831
Category : Science
Languages : en
Pages : 289
Book Description
This volume contains the texts of the nineteen lectures presented at the NATO-ASI - FEBS Course on "DNA - ligand interactions: from drugs to proteins." The Advanced Study Institute (ASIl was held from August 30th to September 11th. 1986 in the Abbey of Fontevraud (France). The ASI was attended by 112 participants from a wide scientific horizon and from twentyone different countries. It was in some way a follow-up of the ASI held in Maratea. Italy in May 1981 and which was published in the NATO ASI Life Science series as volume 45. While much has been learned about the way the cellular machinery maintains and transmits the genetic heritage. as well as how these processes are regulated. little is Known about how the interactions between the various partners involved are taKing place. The interactions of drugs and proteins with nucleic acids are of evident importance in the understanding of these problems. The spectacular advances in recombinant DNA technology and the increased sophistication of biophysical techniques. in particular >:-ray diffraction and nuclear magnetic resonance. have created a scientific environment which is highly promising for the future of research in molecular biology. These advances permH the serious hope that biology on the molecular level may become a r-eality. Some of the contributions at the ASI presented the most recent advances in this e>:citing field.
Structural Studies of DNA Complexes with Minor Groove-binding Drugs
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 414
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 414
Book Description
Drug-Nucleic Acid Interactions
Author:
Publisher: Elsevier
ISBN: 0080496903
Category : Medical
Languages : en
Pages : 740
Book Description
This volume consolidates the key methods for studying ligand-nucleic acid interactions into a convenient source. Techniques that are examined range from biophysical and chemical approaches to methods rooted in molecular and cell biology.
Publisher: Elsevier
ISBN: 0080496903
Category : Medical
Languages : en
Pages : 740
Book Description
This volume consolidates the key methods for studying ligand-nucleic acid interactions into a convenient source. Techniques that are examined range from biophysical and chemical approaches to methods rooted in molecular and cell biology.
DNA Interactions with Drugs and Other Small Ligands
Author: Marcio Santos Rocha
Publisher: Elsevier
ISBN: 0323993850
Category : Medical
Languages : en
Pages : 268
Book Description
DNA Interactions With Drugs and Other Small Ligands: Single Molecule Approaches and Techniques provides the reader with all the main information, a "state-of-the-art" of sorts and an overall review of the field. There is no other book currently available that covers all these subjects together. On the contrary, the different subjects that are developed in this book are currently scattered in journal articles and other books. - Presents a review of the fundamental knowledge, techniques and relevant information surrounding the field of DNA interactions with drugs and other ligands - Provides a resource like no other book available - Includes valuable information from the author who is a highly experienced researcher in the field
Publisher: Elsevier
ISBN: 0323993850
Category : Medical
Languages : en
Pages : 268
Book Description
DNA Interactions With Drugs and Other Small Ligands: Single Molecule Approaches and Techniques provides the reader with all the main information, a "state-of-the-art" of sorts and an overall review of the field. There is no other book currently available that covers all these subjects together. On the contrary, the different subjects that are developed in this book are currently scattered in journal articles and other books. - Presents a review of the fundamental knowledge, techniques and relevant information surrounding the field of DNA interactions with drugs and other ligands - Provides a resource like no other book available - Includes valuable information from the author who is a highly experienced researcher in the field
NMR Studies of DNA and Interactions Between DNA and Ligands
Author: Mogens Hald
Publisher:
ISBN:
Category :
Languages : en
Pages :
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages :
Book Description