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Author: Haralambos Stamatis
Publisher:
ISBN: 9781071622698
Category : Multienzyme complexes
Languages : en
Pages : 379
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Book Description
This volume details cutting-edge methods and protocols for the development, characterization, and applications of multienzyme assemblies. Chapters guide readers through up-to-date techniques applied for the development and emerging applications of multi-enzymatic systems for biotransformations, biosensing, molecular-scale diagnostics and bioelectronics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Multienzymatic Assemblies: Methods and Protocols aims to be useful for academics and industry professionals working in the field of biotechnology, biochemistry, chemical and biological engineering, nanobiotechnology, and biocatalysis.
Author: Haralambos Stamatis
Publisher:
ISBN: 9781071622698
Category : Multienzyme complexes
Languages : en
Pages : 379
Get Book Here
Book Description
This volume details cutting-edge methods and protocols for the development, characterization, and applications of multienzyme assemblies. Chapters guide readers through up-to-date techniques applied for the development and emerging applications of multi-enzymatic systems for biotransformations, biosensing, molecular-scale diagnostics and bioelectronics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Multienzymatic Assemblies: Methods and Protocols aims to be useful for academics and industry professionals working in the field of biotechnology, biochemistry, chemical and biological engineering, nanobiotechnology, and biocatalysis.
Author: Steven E. Cohen (Ph. D.)
Publisher:
ISBN:
Category :
Languages : en
Pages : 148
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Book Description
The Wood-Ljungdahl pathway of acetogenesis allows for growth utilizing carbon dioxide as the sole carbon source. This process uses a series of metalloenzymes to catalyze the reduction of two molecules of carbon dioxide to acetyl-CoA. Key to this pathway are the bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase (CODH/ACS) and corrinoid iron-sulfur protein (CFeSP). CODH/ACS is a bifunctional enzyme, with the CODH subunit catalyzing the reduction of carbon dioxide to carbon monoxide. CFeSP is a cobalt-containing corrinoid-dependent methyltransferase. The active site of ACS, termed the A-cluster, is a nickel, iron, and sulfur-containing metallocluster which catalyzes the synthesis of acetyl-CoA from carbon monoxide provided by CODH, a methyl group provided by CFeSP, and CoA. Despite the unprecedented organometallic chemistry performed at the A-cluster much is unknown about this ACS activity. No substrate-bound structures of ACS have ever been reported and the molecular basis for ACS and CFeSP interactions are largely uncharacterized. Here we present the structure of the carbonylated A-cluster in CODH/ACS, highlighting the role of conformational dynamics in catalysis. We further characterize ACS dynamics using negative-stain electron microscopy, demonstrating a much larger extent of conformational flexibility than has been crystallographically observed. The basis for CFeSP:ACS interactions is further interrogated using a series of CFeSP domain constructs, showing the corrinoidbinding Rossmann fold to be sufficient for ACS interactions. Finally, we report preliminary structural characterization of acetyl-CoA decarbonylase/synthase (ACDS), a 2.2 MDa complex of CODH, ACS, and CFeSP found in archaeal methanogens. Together, this advances our understanding of the roles of structure and dynamics in the Wood-Ljungdahl pathway.
Author: Alasdair Steven
Publisher: Garland Science
ISBN: 1000064905
Category : Medical
Languages : en
Pages : 881
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Book Description
Molecular Biology of Assemblies and Machines provides a comprehensive narrative of the ways in which macromolecular structures assemble and how they interact with other complexes and organelles in the cell. Richly illustrated in full color, the text is written for advanced undergraduates, graduate students, and researchers in biochemistry, molecular biology, biophysics, cell biology, chemistry, structural biology, immunology, microbiology, and medicine.
Author: Jacqueline D. Keighron
Publisher:
ISBN:
Category :
Languages : en
Pages : 182
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Book Description
Author: G. Rickey Welch
Publisher: Elsevier
ISBN: 0323149103
Category : Science
Languages : en
Pages : 473
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Book Description
Organized Multienzyme Systems: Catalytic Properties describes the kinetic and catalytic properties of organized enzyme systems. This book is composed of nine chapters that specifically cover both immobilized and naturally occurring systems. The first two chapters examine the nature and function of enzyme organization in the mitochondrion, as well as the structural/functional coupling of the components in energy-transducing membrane systems. These topics are followed by discussions on ""dynamic compartmentation"" in soluble multienzyme systems; the allosteric enzyme systems; and allosterism in reversibly adsorptive enzyme systems. Other chapters explore model studies with specific immobilized multienzyme sequences, as regards the analysis of microenvironmental effects, and the mathematical exposition on the kinetic analysis of multienzyme systems in homogeneous solution. The last chapters present some theoretical and experimental studies on the behavior of immobilized systems. These chapters also provide a speculative integrative view of the kind of functional coherence that may be operative in organized states in vivo. This book is of great value to cell biologists, biochemists, and enzyme scientists and researchers.
Author:
Publisher: Academic Press
ISBN: 0128170751
Category : Science
Languages : en
Pages : 511
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Book Description
Metabolons and Supramolecular Enzyme Assemblies, Volume 617 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Dynamic plant metabolons, TCA cycle metabolons, the Chemotactic assembly of metabolons, Repurposing peroxisomes for metabolic engineering, Repurposing yeast mitochondria for metabolic engineering, Repurposing plant compartments for metabolic engineering, Protein scaffolds for pathway co-localization on lipid droplets, Engineered enzyme assemblies for metabolic engineering, NRPS assembly lines and P450 interactions, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in this series on enzymology Updated release includes the latest information on dynamic plant metabolons, TCA cycle metabolons, the chemotactic assembly of metabolons, and much more
Author: Harry Gilbert
Publisher: Academic Press
ISBN: 0124159311
Category : Medical
Languages : en
Pages : 564
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Book Description
Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyse cellulolysis. This volume covers subjects such as 'the DNSA reducing assay for measuring cellulases', 'measuring processivity' and 'in situ cellulose detection with carbohydrate-binding modules'.
Author:
Publisher: ScholarlyEditions
ISBN: 1464945977
Category : Medical
Languages : en
Pages : 32
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Book Description
Multienzyme Complexes: Advances in Research and Application: 2011 Edition is a ScholarlyPaper™ that delivers timely, authoritative, and intensively focused information about Multienzyme Complexes in a compact format. The editors have built Multienzyme Complexes: Advances in Research and Application: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Multienzyme Complexes in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Multienzyme Complexes: Advances in Research and Application: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Author: Chunhai Fan
Publisher: Springer Science & Business Media
ISBN: 3642360777
Category : Technology & Engineering
Languages : en
Pages : 361
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Book Description
DNA nanotechnology: From structure to function presents an overview of various facets of DNA nanotechnology, with a particular focus on their promising applications. This book is composed of three parts. Part I, Elements of DNA Nanotechnology, provides extensive basic information on DNA nanotechnology. Part II, Static and Dynamic DNA Nanotechnology, describes the design and fabrication of static and dynamic DNA nanostructures. Recent advances in DNA origami, DNA walkers and DNA nanodevices are all covered in this part. Part III, Applications of DNA Nanotechnology, introduces a variety of applications of DNA nanotechnology, including biosensing, computation, drug delivery, etc. Together these provide a comprehensive overview of this emerging area and its broad impact on biological and medical sciences. This book is intended for post-graduates, post-doctoral researchers and research scientists who are interested in expanding their knowledge of DNA nanotechnology. It provides readers an impression of the latest developments in this exciting filed.
Author: Florian Xavier Delbart
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
