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Author: Sanjiv Sam Gambhir
Publisher: Cambridge University Press
ISBN: 1139489526
Category : Science
Languages : en
Pages :
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Book Description
Reporter genes have been used for several decades to study regulation of gene expression in vivo. However, it was little more than a decade ago that a new class of reporter genes was developed for imaging molecular events within living subjects. By following the interactions of protein molecules, researchers can resolve the complex chemical pathways that living cells utilise. This book focuses on this group of imaging reporter genes, starting with detailed descriptions of all reporter genes from different imaging modalities, including optical, MRI, and radionuclide-based imaging. Key scientists in the field explain how to enhance reporter gene imaging utility through instrumentation and the various applications of this technology. This is the first comprehensive book on all aspects of reporter gene imaging, detailing what is known in the field and future goals for research. Investigators in biomedical sciences, physicians, and the biotechnology and pharmaceutical industries will benefit from topics covered.
Author: Sanjiv Sam Gambhir
Publisher: Cambridge University Press
ISBN: 1139489526
Category : Science
Languages : en
Pages :
Get Book Here
Book Description
Reporter genes have been used for several decades to study regulation of gene expression in vivo. However, it was little more than a decade ago that a new class of reporter genes was developed for imaging molecular events within living subjects. By following the interactions of protein molecules, researchers can resolve the complex chemical pathways that living cells utilise. This book focuses on this group of imaging reporter genes, starting with detailed descriptions of all reporter genes from different imaging modalities, including optical, MRI, and radionuclide-based imaging. Key scientists in the field explain how to enhance reporter gene imaging utility through instrumentation and the various applications of this technology. This is the first comprehensive book on all aspects of reporter gene imaging, detailing what is known in the field and future goals for research. Investigators in biomedical sciences, physicians, and the biotechnology and pharmaceutical industries will benefit from topics covered.
Author: Sanjiv Sam Gambhir
Publisher:
ISBN: 9780511729898
Category : Medical
Languages : en
Pages : 337
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Book Description
Focuses on the imaging of reporter genes in living subjects, a technology that was developed only ten years ago.
Author: Purnima Dubey
Publisher: Humana
ISBN: 9781493993062
Category : Medical
Languages : en
Pages : 221
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Book Description
This volume provides an introduction for the scientist who is new to the field of molecular imaging, as well as detailed methods for experts in other areas of molecular imaging. Chapters detail the advantages and limitations of combining fluorescent, bioluminescent and radioisotopic, creating dual modality imaging reporter gene construct, bioluminescent imaging, and bioluminescent or fluorescent imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Reporter Gene Imaging : Methods and Protocols aims to ensure successful results in the further study of this vital field.
Author: Donald S. Anson
Publisher: Springer Science & Business Media
ISBN: 158829739X
Category : Science
Languages : en
Pages : 165
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Book Description
Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. In Reporter Genes: A Practical Guide, renowned researchers describe practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available, concentrating on those marker genes that are currently most commonly used. Among the topics covered in this volume are methodologies for staining and visualization of ß-galactosidase in embryos and tissues, immunohistochemical detection of ß-galactosidase, detection of reporter gene expression in murine airways, and three dimensional analysis of molecular signals with episcopic imaging techniques. Also covered in this groundbreaking text are fluorescent proteins, detection of GFP during nervous system development, fluorescent protein reporter systems for single cell measurements, and non-invasive imaging of molecular events. Comprehensive and illuminating, Reporter Genes: A Practical Guide will be an oft-used reference for geneticists, molecular engineers, and molecular biologists.
Author: Robert Damoiseaux
Publisher:
ISBN: 9781493977246
Category : Reporter genes
Languages : en
Pages : 234
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Book Description
Author: Zachary Floyd Walls
Publisher:
ISBN:
Category :
Languages : en
Pages : 406
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Book Description
Author: Ziyue Jiang
Publisher:
ISBN:
Category :
Languages : en
Pages : 106
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Book Description
The advanced stage of prostate cancer is the second leading cause of cancer-related death for American men. Novel, effective treatment options and more cancer-specific diagnostic tools are urgently needed to facilitate patient management. Here, we explored the construction and application of an array of gene-based molecular imaging and therapeutic vectors in a variety of clinically relevant settings. These vectors exploit prostate cancer-specific promoters to control the transcription of imaging reporter genes or therapeutic genes, and thus can achieve stringent cancer selectivity. We showed that gene-based imaging vectors can detect prostate cancer metastasis earlier than conventional imaging modalities; we also developed a dual-reporter imaging system that can specifically interrogate the activation/inhibition status of androgen receptor, and thus monitor the efficacy of androgen deprivation and androgen receptor blockade therapies. Further, we explored the applicability of gene-based vectors in detecting circulating tumor cells and have obtained promising results. Moreover, we employed two strategies to improve the clinical usability of viral vectors in immunocompetent hosts (such as humans). In the first strategy, we used a broad-acting immunosuppressant, rapamycin, to reduce the scale of adenovirus-induced inflammatory responses and also to decrease the eliminating effects of host adaptive immune system on viral vectors and viral-infected, transgene-expressing cells. Rapamycin significantly augmented the magnitude and prolonged the duration of virus-delivered transgene expression, and thus enhanced the diagnostic capability of these imaging vectors. The second approach took advantage of the versatile surface modifying ability of non-viral reagents. We designed a polypeptide coating that can non-covalently bind to adenoviral particles and confer protections against blood components such as neutralizing antibodies and coagulation factors. The coating significantly decreased the inhibitory effects of neutralizing antibodies on adenoviral transduction and markedly abrogated coagulation factors-mediated hepatocyte infection. Furthermore, this polypeptide coating reduced the antigenicity of adenoviral vectors in vivo, greatly diminished anti-adenovirus antibody production, and therefore could facilitate repeated administration of viral vectors in immunocompetent hosts. Overall, we herein present a substantial amount of evidence supporting the pre-clinical and translational use of gene-based imaging and therapeutic vectors to assist the management of patients with advanced, treatment-refractory prostate cancer.
Author: Don Anson
Publisher: Humana Press
ISBN: 9781588297396
Category : Science
Languages : en
Pages : 158
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Book Description
Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. This book describes practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available.
Author: Mousumi Debnath
Publisher: Springer Science & Business Media
ISBN: 9048132614
Category : Medical
Languages : en
Pages : 527
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Book Description
A rapid development in diverse areas of molecular biology and genetic engineering resulted in emergence of variety of tools. These tools are not only applicable to basic researches being carried out world over, but also exploited for precise detection of abnormal conditions in plants, animals and human body. Although a basic researcher is well versed with few techniques used by him/her in the laboratory, they may not be well acquainted with methodologies, which can be used to work out some of their own research problems. The picture is more blurred when the molecular diagnostic tools are to be used by physicians, scientists and technicians working in diagnostic laboratories in hospitals, industry and academic institutions. Since many of them are not trained in basics of these methods, they come across several gray areas in understanding of these tools. The accurate application of molecular diagnostic tools demands in depth understanding of the methodology for precise detection of the abnormal condition of living body. To meet the requirements of a good book on molecular diagnostics of students, physicians, scientists working in agricultural, veterinary, medical and pharmaceutical sciences, it needs to expose the reader lucidly to: Give basic science behind commonly used tools in diagnostics Expose the readers to detailed applications of these tools and Make them aware the availability of such diagnostic tools The book will attract additional audience of pathologists, medical microbiologists, pharmaceutical sciences, agricultural scientists and veterinary doctors if the following topics are incorporated at appropriate places in Unit II or separately as a part of Unit-III in the book. Molecular diagnosis of diseases in agricultural crops Molecular diagnosis of veterinary diseases. Molecular epidemiology, which helps to differentiate various epidemic strains and sources of disease outbreaks. Even in different units of the same hospital, the infections could be by different strains of the same species and the information becomes valuable for infection control strategies. Drug resistance is a growing problem for bacterial, fungal and parasitic microbes and the molecular biology tools can help to detect the drug resistance genes without the cultivation and in vitro sensitivity testing. Molecular diagnostics offers faster help in the selection of the proper antibiotic for the treatment of tuberculosis, which is a major problem of the in the developing world. The conventional culture and drug sensitivity testing of tuberculosis bacilli is laborious and time consuming, whereas molecular diagnosis offers rapid drug resistant gene detection even from direct clinical samples. The same approach for HIV, malaria and many more diseases needs to be considered. Molecular diagnostics in the detection of diseases during foetal life is an upcoming area in the foetal medicine in case of genetic abnormalities and infectious like TORCH complex etc. The book will be equally useful to students, scientists and professionals working in the field of molecular diagnostics.
Author: Donna Goldhawk Goldhawk
Publisher:
ISBN:
Category : Electronic books
Languages : en
Pages : 0
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Book Description
Nanoparticle research has greatly benefitted medical imaging platforms by generating new signals, enhancing detection sensitivity, and expanding both clinical and preclinical applications. For magnetic resonance imaging, the fabrication of superparamagnetic iron oxide nanoparticles has provided a means of detecting cells and has paved the way for magnetic particle imaging. As the field of molecular imaging grows and enables the tracking of cells and their molecular activities so does the possibility of tracking genetically programmed biomarkers. This chapter discusses the advantages and challenges of gene-based contrast, using the bacterial magnetosome model to highlight the requirements of in vivo iron biomineralization and reporter gene expression for magnetic resonance signal detection. New information about magnetosome protein interactions in non-magnetic mammalian cells is considered in the light of design and application(s) of a rudimentary magnetosome-like nanoparticle for molecular imaging. Central to this is the hypothesis that a magnetosome root structure is defined by essential magnetosome genes, whose expression positions the biomineral in a given membrane compartment, in any cell type. The use of synthetic biology for programming multi-component structures not only broadens the scope of reporter gene expression for molecular MRI but also facilitates the tracking of cell therapies.
