Author: Qi Sun
Publisher:
ISBN:
Category : Electronic dissertations
Languages : en
Pages : 382
Book Description
Molecular Genetics of Axon Guidance in Drosophila Melanogaster
Author: Qi Sun
Publisher:
ISBN:
Category : Electronic dissertations
Languages : en
Pages : 382
Book Description
Publisher:
ISBN:
Category : Electronic dissertations
Languages : en
Pages : 382
Book Description
A Molecular Genetic Analysis of Axon Guidance in Drosophila Melanogaster
Author: Huidy Shu
Publisher:
ISBN:
Category :
Languages : en
Pages : 336
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 336
Book Description
A Molecular Genetic Analysis of Motoneuron Axon Guidance in Drosophila Melanogaster
Author: Gordon Clark Teg Pipes
Publisher:
ISBN:
Category :
Languages : en
Pages : 338
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 338
Book Description
A Molecular Genetic Analysis of Target Selection in Drosophila Melanogaster
Author: Roger Chi-Che Lee
Publisher:
ISBN:
Category :
Languages : en
Pages : 264
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 264
Book Description
A Molecular Genetic Analysis of the Role of the Guanine Nucleotide Exchange Factor Trio During Axon Pathfinding in the Embryonic CNS of Drosophila Melanogaster
Author: David J. Forsthoefel
Publisher:
ISBN:
Category : Actin
Languages : en
Pages :
Book Description
Abstract: The Drosophila melanogaster embryo is an ideal system in which to study axon guidance, because of the relative simplicity of the nervous system and the evolutionary conservation of the molecules utilized during development. The Abelson cytoplasmic tyrosine kinase regulates actin cytoskeletal dynamics in Drosophila, mice, and humans. In Drosophila, Abl is expressed in the developing central and peripheral nervous systems (CNS and PNS). In a genetic screen for modifiers of the Abl mutant semilethality phenotype, we identified trio, a cytoplasmic guanine nucleotide exchange factor that is also expressed in the CNS and regulates actin dynamics through Rho GTPases. Mutations in Abl and trio interacted genetically, leading to dramatic disruption of axon pathways at the CNS midline. Building upon these initial observations, we analyzed interactions between Abl, trio, and the attractive Netrin receptor frazzled (fra)/Deleted-in-Colorectal-Cancer (DCC). In fra;Abl and fra;trio double mutants, few axons crossed the midline, similar to the phenotype in trio, Abl mutants. Furthermore, mutations in Abl and trio suppressed the inappropriate midline crossover phenotype in embryos expressing the chimeric Robo-Fra receptor, consistent with an in vivo role for these molecules as Fra effectors. Fra bound Abl and Trio in coimmunoprecipitation and GST pulldown experiments, and tyrosine phosphorylation of Fra and Trio was elevated in cultured cells overexpressing Abl. Mutations in enabled (ena), another Abl substrate, suppressed the loss-of-commissure phenotype in fra, Abl, and trio mutants, as well as the Robo-Fra receptor phenotype. Together, these results suggest that Abl and Trio are effectors for multiple attractive receptors at the CNS midline, and that Ena may function during both attractive and repulsive signaling. Finally, a functional analysis of the requirement for Trio's conserved domains has been initiated. In transgenic rescue and overexpression experiments, TrioGEF1 was required for axon guidance across the CNS midline, while TrioSH3 inhibited midline crossing. Coexpression experiments with the Robo-Fra receptor and assays in other tissues and cultured cells suggest that the conserved N-terminal domain, spectrin-like repeats, and GEF2 domain may modulate GEF1 signaling in specific contexts. Future experiments must elucidate the mechanistic details of cytoskeletal control by Trio and Fra.
Publisher:
ISBN:
Category : Actin
Languages : en
Pages :
Book Description
Abstract: The Drosophila melanogaster embryo is an ideal system in which to study axon guidance, because of the relative simplicity of the nervous system and the evolutionary conservation of the molecules utilized during development. The Abelson cytoplasmic tyrosine kinase regulates actin cytoskeletal dynamics in Drosophila, mice, and humans. In Drosophila, Abl is expressed in the developing central and peripheral nervous systems (CNS and PNS). In a genetic screen for modifiers of the Abl mutant semilethality phenotype, we identified trio, a cytoplasmic guanine nucleotide exchange factor that is also expressed in the CNS and regulates actin dynamics through Rho GTPases. Mutations in Abl and trio interacted genetically, leading to dramatic disruption of axon pathways at the CNS midline. Building upon these initial observations, we analyzed interactions between Abl, trio, and the attractive Netrin receptor frazzled (fra)/Deleted-in-Colorectal-Cancer (DCC). In fra;Abl and fra;trio double mutants, few axons crossed the midline, similar to the phenotype in trio, Abl mutants. Furthermore, mutations in Abl and trio suppressed the inappropriate midline crossover phenotype in embryos expressing the chimeric Robo-Fra receptor, consistent with an in vivo role for these molecules as Fra effectors. Fra bound Abl and Trio in coimmunoprecipitation and GST pulldown experiments, and tyrosine phosphorylation of Fra and Trio was elevated in cultured cells overexpressing Abl. Mutations in enabled (ena), another Abl substrate, suppressed the loss-of-commissure phenotype in fra, Abl, and trio mutants, as well as the Robo-Fra receptor phenotype. Together, these results suggest that Abl and Trio are effectors for multiple attractive receptors at the CNS midline, and that Ena may function during both attractive and repulsive signaling. Finally, a functional analysis of the requirement for Trio's conserved domains has been initiated. In transgenic rescue and overexpression experiments, TrioGEF1 was required for axon guidance across the CNS midline, while TrioSH3 inhibited midline crossing. Coexpression experiments with the Robo-Fra receptor and assays in other tissues and cultured cells suggest that the conserved N-terminal domain, spectrin-like repeats, and GEF2 domain may modulate GEF1 signaling in specific contexts. Future experiments must elucidate the mechanistic details of cytoskeletal control by Trio and Fra.
Genetic Analysis of Axon Guidance in Drosophila Melanogaster
Author: Ashley Palani Wright
Publisher:
ISBN:
Category : Drosophila
Languages : en
Pages : 0
Book Description
Publisher:
ISBN:
Category : Drosophila
Languages : en
Pages : 0
Book Description
A Molecular and Genetic Analysis of Neuromuscular Connectivity and Synaptic Growth in Drosophila Melanogaster
Author: Hong Iris Wan
Publisher:
ISBN:
Category :
Languages : en
Pages : 392
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 392
Book Description
A Genetic and Molecular Analysis of Neuromuscular Connectivity in Drosophila Melanogaster
Author: Douglas McIntosh Fambrough
Publisher:
ISBN:
Category :
Languages : en
Pages : 526
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 526
Book Description
Muscle Development in Drosophilia
Author: Helen Sink
Publisher: Springer Science & Business Media
ISBN: 0387329633
Category : Science
Languages : en
Pages : 214
Book Description
The different aspects of muscle development are considered from cellular, molecular and genetic viewpoints, and the text is supported by black/white and color illustrations. The book will appeal to those studying muscle development and muscle biology in any organism.
Publisher: Springer Science & Business Media
ISBN: 0387329633
Category : Science
Languages : en
Pages : 214
Book Description
The different aspects of muscle development are considered from cellular, molecular and genetic viewpoints, and the text is supported by black/white and color illustrations. The book will appeal to those studying muscle development and muscle biology in any organism.
Function and Regulation of the Axon Guidance Molecules Sidestep and Beaten Path Ia in Drosophila Melanogaster
Author: Jaqueline Kinold
Publisher:
ISBN:
Category :
Languages : en
Pages :
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages :
Book Description