Molecular-cytogenetic Characterization of Head and Neck Cancer PDF Download
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Author: Volkert B. Wreesmann
Publisher:
ISBN:
Category : Chromosome abnormalities
Languages : en
Pages : 516
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Book Description
Academic dissertation for obtaining the degree of doctor by the University of Amsterdam under the authority of Rector Magnificus prof. mr. P.F. van der Heijden in the presence of the regular promotion committee to defend in public in the Auditorium of the University on thursday 2 december 2004, at 10:30 hour with Volkert Boudewijn Wreesmann born in Rotterdam and at 11:30 hours with Bhuvanesh Singh born in Patna (India).
Author: Volkert B. Wreesmann
Publisher:
ISBN:
Category : Chromosome abnormalities
Languages : en
Pages : 516
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Book Description
Academic dissertation for obtaining the degree of doctor by the University of Amsterdam under the authority of Rector Magnificus prof. mr. P.F. van der Heijden in the presence of the regular promotion committee to defend in public in the Auditorium of the University on thursday 2 december 2004, at 10:30 hour with Volkert Boudewijn Wreesmann born in Rotterdam and at 11:30 hours with Bhuvanesh Singh born in Patna (India).
Author: Volkert Boudewijn Wreesmann
Publisher:
ISBN:
Category :
Languages : en
Pages : 506
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Book Description
Author: Jerry Machado
Publisher:
ISBN: 9780494731468
Category :
Languages : en
Pages : 594
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Book Description
Head and neck squamous cell carcinomas (HNSCCs) most commonly develop in older patients (≥60 years of age) with a history of tobacco and alcohol use. However, young individuals (≥45 years of age) can also develop HNSCC, often without common risk factors. Increasing evidence shows that Human Papillomavirus (HPV) infection is associated with particular HNSCC sites (e.g. oropharynx). We assessed the Roche Linear Array HPV Genotyping Test in several lesions and then examined the prevalence of HPV in HNSCCs from young and older patients. HPV infection was most prevalent in oropharyngeal cancers (16/22, 73%), rarely found in oral cavity cancers (2/53, 4%), and other head and neck sites (1/17, 6%). HPV positive tumors were associated with patients that were >40 and 60 years old (p=0.02).The absence or shortened time of carcinogen exposure from common risk factors and the development of oral squamous cell carcinoma (OSCC) at an early age suggest aberrant genetic events that are different than those in OSSCs from older patients. We used Affymetrix SNP 6.0 arrays to genomically profile oral tumors from young and older patients. Tumors from young patients showed different regions/genes of copy number alterations than those from older patient tumors. An increase of regions of loss of heterozygosity (LOH) in tumors from older patients was observed, and there was a high prevalence of copy number neutral LOH on chromosome 9 in tumors from young and older patients. These data suggest different genetic mechanisms in these patient groups.We have previously shown that HNSCCs from younger patients exhibited a high incidence of microsatellite instability (MSI), a marker of defective mismatch repair (MMR). Deregulated mRNA levels of hPMS1, hPMS2 and hMLH1 were observed and absent/low expression of hPMS1, hPMS2 and hMLH1 protein levels were observed in 50% of OSCCs. No mutations were observed in hPMS1 and hPMS2 and no significant differences of MSI or LOH were observed across genomic loci between tumors of young and older patients. The role of these genetic mechanisms in oral cancer appears complex; studies such as ours should further improve our knowledge of the molecular mechanisms leading to early-onset oral carcinomas.
Author: 錢文偉
Publisher: Open Dissertation Press
ISBN: 9781374770621
Category : Medical
Languages : en
Pages : 116
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Book Description
This dissertation, "Cytogenetic Analysis of Head and Neck Cancer by Comparative Genomic Hybridization" by 錢文偉, Man-wai, Gary, Chien, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. DOI: 10.5353/th_b3122420 Subjects: Head - Cancer - Genetic aspects Neck - Cancer - Genetic aspects Cell hybridization
Author: Man-wai Chien (Gary)
Publisher:
ISBN:
Category : Cell hybridization
Languages : en
Pages : 206
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Author:
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
The incidence of papillary thyroid carcinoma (PTC) increases significantly after exposure of the head and neck region to ionizing radiation, yet we know neither the steps involved in malignant transformation of thyroid epithelium nor the specific carcinogenic mode of action of radiation. Such increased tumor frequency became most evident in children after the 1986 nuclear accident in Chernobyl, Ukraine. In the twelve years following the accident, the average incidence of childhood PTCs (chPTC) increased over one hundred-fold compared to the rate of about 1 tumor incidence per 106 children per year prior to 1986. To study the etiology of radiation-induced thyroid cancer, we formed an international consortium to investigate chromosomal changes and altered gene expression in cases of post-Chernobyl chPTC. Our approach is based on karyotyping of primary cultures established from chPTC specimens, establishment of cell lines and studies of genotype-phenotype relationships through high resolution chromosome analysis, DNA/cDNA micro-array studies, and mouse xenografts that test for tumorigenicity. Here, we report the application of fluorescence in situ hybridization (FISH)-based techniques for the molecular cytogenetic characterization of a highly tumorigenic chPTC cell line, S48TK, and its subclones. Using chromosome 9 rearrangements as an example, we describe a new approach termed ''BAC-FISH'' to rapidly delineate chromosomal breakpoints, an important step towards a better understanding of the formation of translocations and their functional consequences.
Author: Tali Shani
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Marilyn S. Arsham
Publisher: John Wiley & Sons
ISBN: 1119061229
Category : Science
Languages : en
Pages : 1216
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Book Description
Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics. Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization (FISH) and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive description of the diagnostic tests offered by the clinical laboratory and explains the science behind them. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters by experts in the field. Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN’s cytogenetic language to describe chromosome changes; tips for laboratory management; examples of laboratory information systems; a collection of internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the basis of each of these procedures. This makes it a useful resource for researchers, clinicians, and lab professionals, as well as students in a university or medical school setting.
Author: Sverre Heim
Publisher: John Wiley & Sons
ISBN: 1118210581
Category : Medical
Languages : en
Pages : 799
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Book Description
Cancer Cytogenetics, 3rd Edition, offers a comprehensive, expanded, and up-to-date review of recent dramatic advances in this area and incorporates a vast amount of new data from the latest basic and clinical investigations. Edited by two leading experts, and now involving a new panel of international experts, the book offers an authoritative description of neoplastic processes at the chromosomal level of genomic organization. Researchers in cytogenetics, medical and molecular genetics, cellular and molecular biology, cancer research, clinical oncology, and hematology will find this reference both thorough and authoritative.
Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1666
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Book Description
Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
