Microrheology with Optical Tweezers PDF Download
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Author: Manlio Tassieri
Publisher: CRC Press
ISBN: 9814669199
Category : Science
Languages : en
Pages : 329
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Book Description
Thanks to the pioneering works of Ashkin and coworkers, optical tweezers (OTs) have become an invaluable tool for myriad studies throughout the natural sciences. Their success relies on the fact that they can be considered as exceptionally sensitive transducers that are able to resolve pN forces and nm displacements, with high temporal resolution, down to μs. Hence their application to study a wide range of biological phenomena such as measuring the compliance of bacterial tails, the forces exerted by a single motor protein, and the mechanical properties of human red blood cells and of individual biological molecules. The number of articles related to them totals to a whopping 58,000 (source Google Scholar)! Microrheology is a branch of rheology, but it works at micrometer length scales and with microliter sample volumes. Therefore, microrheology techniques have been revealed to be very useful tools for all those rheological/mechanical studies where rare or precious materials are employed, such as in biological and biomedical studies. The aim of this book is to provide a pedagogical introduction to the physics principles governing both the optical tweezers and their application in the field of microrheology of complex materials. This is achieved by following a linear path that starts from a narrative introduction of the "nature of light," followed by a rigorous description of the fundamental equations governing the propagation of light through matter. Moreover, some of the many possible instrumental configurations are presented, especially those that better adapt to perform microrheology measurements. In order to better appreciate the microrheological methods with optical tweezers explored in this book, informative introductions to the basic concepts of linear rheology, statistical mechanics, and the most popular microrheology techniques are also given. Furthermore, an enlightening prologue to the general applications of optical tweezers different from rheological purposes is provided at the end of the book.
Author: Manlio Tassieri
Publisher: CRC Press
ISBN: 9814669199
Category : Science
Languages : en
Pages : 329
Get Book Here
Book Description
Thanks to the pioneering works of Ashkin and coworkers, optical tweezers (OTs) have become an invaluable tool for myriad studies throughout the natural sciences. Their success relies on the fact that they can be considered as exceptionally sensitive transducers that are able to resolve pN forces and nm displacements, with high temporal resolution, down to μs. Hence their application to study a wide range of biological phenomena such as measuring the compliance of bacterial tails, the forces exerted by a single motor protein, and the mechanical properties of human red blood cells and of individual biological molecules. The number of articles related to them totals to a whopping 58,000 (source Google Scholar)! Microrheology is a branch of rheology, but it works at micrometer length scales and with microliter sample volumes. Therefore, microrheology techniques have been revealed to be very useful tools for all those rheological/mechanical studies where rare or precious materials are employed, such as in biological and biomedical studies. The aim of this book is to provide a pedagogical introduction to the physics principles governing both the optical tweezers and their application in the field of microrheology of complex materials. This is achieved by following a linear path that starts from a narrative introduction of the "nature of light," followed by a rigorous description of the fundamental equations governing the propagation of light through matter. Moreover, some of the many possible instrumental configurations are presented, especially those that better adapt to perform microrheology measurements. In order to better appreciate the microrheological methods with optical tweezers explored in this book, informative introductions to the basic concepts of linear rheology, statistical mechanics, and the most popular microrheology techniques are also given. Furthermore, an enlightening prologue to the general applications of optical tweezers different from rheological purposes is provided at the end of the book.
Author: Kenny Breuer
Publisher: Springer Science & Business Media
ISBN: 3540264493
Category : Technology & Engineering
Languages : en
Pages : 268
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Book Description
Microscale Diagnostic Techniques highlights the most innovative and powerful developments in microscale diagnostics. It provides a resource for scientists and researchers interested in learning about the techniques themselves, including their capabilities and limitations. The fields of Micro- and Nanotechnology have emerged over the past decade as a major focus of modern scientific and engineering research and technology. Driven by advances in microfabrication, the investigation, manipulation and engineering of systems characterized by micrometer and, more recently, nanometer scales have become commonplace throughout all technical disciplines. With these developments, an entirely new collection of experimental techniques has been developed to explore and characterize such systems.
Author: Miles J. Padgett
Publisher: CRC Press
ISBN: 1420074148
Category : Science
Languages : en
Pages : 510
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Book Description
The technical development of optical tweezers, along with their application in the biological and physical sciences, has progressed significantly since the demonstration of an optical trap for micron-sized particles based on a single, tightly focused laser beam was first reported more than twenty years ago. Bringing together many landmark papers on
Author: Philip H. Jones
Publisher: Cambridge University Press
ISBN: 1107051169
Category : Science
Languages : en
Pages : 565
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Book Description
A comprehensive guide to the theory, practice and applications of optical tweezers, combining state-of-the-art research with a strong pedagogic approach.
Author: D.R. Oliver
Publisher: Springer
ISBN: 9789401068383
Category : Science
Languages : en
Pages : 568
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Book Description
Author: Reinhard Miller
Publisher: CRC Press
ISBN: 9004175865
Category : Science
Languages : en
Pages : 699
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Book Description
This is the first book on interfacial rheology. It aims to describe both its history as well as the current, most frequently used experimental techniques for studying dilational and shear rheology of layers at liquid/gas and liquid/liquid interfaces. The book opens with a chapter on the fundamentals of interfacial rheology. All (16) contributions include the theoretical basis for the presented methodologies, and experimental examples are given.
Author: J.-B. Suck
Publisher: Springer Science & Business Media
ISBN: 3662050285
Category : Science
Languages : en
Pages : 575
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Book Description
The book provides an introduction to all aspects of the physics of quasicrystals. The chapters, each written by an expert in this field, cover quasiperiodic tilings and the modeling of the atomic structure of quasicrystals. The electronic density of states and the calculation of the electronic structure play a key role in this introduction, as does an extensive discussion of the atomic dynamics. The study of defects in quasicrystals by high resolution electron microscopy and the computer simulations of defects and fracture in decorated tilings are important subjects for the application of these aperiodic crystals.
Author: Eric M. Furst
Publisher: Oxford University Press
ISBN: 0199655200
Category : Science
Languages : en
Pages : 473
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Book Description
Rheology is the study of the flow of matter. It is an important and active field of research that spans numerous disciplines and technological applications. The aim of this work is to provide an introduction to the theory and practice of microrheology, a relatively new area of rheology.
Author: Arne Gennerich
Publisher: Humana
ISBN: 9781493964192
Category : Science
Languages : en
Pages : 0
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Book Description
The aim of this volume is to provide a comprehensive overview of optical tweezers setups, both in practical and theoretical terms, to help biophysicists, biochemists, and cell biologists to build and calibrate their own instruments and to perform force measurements on mechanoenzymes both in isolation in vitro and in living cells. Chapters have been divided in three parts focusing on theory and practical design of optical tweezers, detailed protocols for performing force measurements on single DNA- and microtubule/actin-associated mechanoenzymes in isolation, and describing recent advances that have opened up quantitative force measurements in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Optical Tweezers: Methods and Protocols aims help to further expand the accessibility and use of optical traps by scientists of diverse disciplines.
Author: Federico Toschi
Publisher: Springer Nature
ISBN: 3030233707
Category : Science
Languages : en
Pages : 313
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Book Description
This open access book, published in the Soft and Biological Matter series, presents an introduction to selected research topics in the broad field of flowing matter, including the dynamics of fluids with a complex internal structure -from nematic fluids to soft glasses- as well as active matter and turbulent phenomena. Flowing matter is a subject at the crossroads between physics, mathematics, chemistry, engineering, biology and earth sciences, and relies on a multidisciplinary approach to describe the emergence of the macroscopic behaviours in a system from the coordinated dynamics of its microscopic constituents. Depending on the microscopic interactions, an assembly of molecules or of mesoscopic particles can flow like a simple Newtonian fluid, deform elastically like a solid or behave in a complex manner. When the internal constituents are active, as for biological entities, one generally observes complex large-scale collective motions. Phenomenology is further complicated by the invariable tendency of fluids to display chaos at the large scales or when stirred strongly enough. This volume presents several research topics that address these phenomena encompassing the traditional micro-, meso-, and macro-scales descriptions, and contributes to our understanding of the fundamentals of flowing matter. This book is the legacy of the COST Action MP1305 “Flowing Matter”.
