Microprobe Analysis in Biology and Medicine PDF Download
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Author: Patrick Echlin
Publisher:
ISBN:
Category : Biology
Languages : en
Pages : 400
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Book Description
Author: Patrick Echlin
Publisher:
ISBN:
Category : Biology
Languages : en
Pages : 400
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Book Description
Author: Peter Ingram
Publisher: Academic Press
ISBN: 0080524567
Category : Science
Languages : en
Pages : 573
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Book Description
Biomedical Applications of Microprobe Analysis is a combination reference/laboratory manual for the use of microprobe analysis in both clinical diagnostic and research settings. Also called microchemical microscopy, microprobe analysis uses high-energy bombardment of cells and tissue, in combination with high resolution EM or confocal microscopy to provide a profile of the ion, metal, and mineral concentrations present in a sample. This allows insight into the physiology and pathophysiology of a wide variety of cells and tissues.This book describes methods for obtaining detailed information about the identity and composition of particles too small to be seen with the naked eye and describes how this information can be useful in diagnostic and biomedical research. - Up-to-date review of electron microprobe analysis - Detailed descriptions of sample preparation techniques - Recent technologies including confocal microscopy, infrared microspectroscopy, and laser raman spectroscopy - Over 100 illustrations with numerous specific applications - Contributions by world-renowned experts in the field - Brief summary of highlights precedes each chapter
Author: Karl Zierold
Publisher: Springer Science & Business Media
ISBN: 364274477X
Category : Science
Languages : en
Pages : 331
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Book Description
The aim of electron probe microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure and composition. In particular, energy spectroscopy of X-rays and electrons after interaction of the electron beam with the specimen is used for this purpose. However, the application of this method in biology and medicine has to overcome three specific problems: 1. The principle constituent of most cell samples is water. Since liquid water is not compatible with vacuum conditions in the electron microscope, specimens have to be prepared without disturbing the other components, in parti cular diffusible ions (elements). 2. Electron probe microanaly sis provides physical data on either dry specimens or fully hydrated, frozen specimens. This data usually has to be con verted into quantitative data meaningful to the cell biologist or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo gical processes requires sampling techniques which are adapted to address specific biological or medical questions. During recent years, remarkable progress has been made to overcome these problems. Cryopreparation, image analysis, and electron energy loss spectroscopy are key areas which have solved some problems and offer promise for future improvements.
Author: David C. Sigee
Publisher: Cambridge University Press
ISBN: 9780521415309
Category : Medical
Languages : en
Pages : 356
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Book Description
This book describes an integrated approach to the use of X-ray microanalysis in biology.
Author: Lylia Khassanova
Publisher: John Libbey Eurotext
ISBN: 9782742004294
Category : Medical
Languages : en
Pages : 692
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Book Description
Proceedings of the Seventh International Symposium on Metal Ions in Biology & Medicine held in Saint Petersburg State University, Saint Petersburg, Russia, on 5-9 May 2002.
Author: Yvan Llabador
Publisher: World Scientific
ISBN: 9789810223625
Category : Science
Languages : en
Pages : 292
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Book Description
"... this book will be particularly useful to the many biologists encountering problems of elemental analysis & analytical imaging who wish to have a clear yet comprehensive introduction to the principles, the conditions of application & the potential of nuclear microprobes compared with those of alternative methods." Journal of Trace & Microprobe Techniques, 1999
Author:
Publisher:
ISBN:
Category : Pathological laboratories
Languages : en
Pages : 40
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Book Description
Author: Claude Lechene
Publisher: Elsevier
ISBN: 0323143342
Category : Nature
Languages : en
Pages : 695
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Book Description
Microbeam Analysis in Biology contains the proceedings of a workshop on Biological X-Ray Microanalysis by Electron Beam Excitation, held in Boston, Massachusetts on August 25-26, 1977. This book focuses on the principles, techniques, and biological use of electron probe microanalysis, energy-loss spectroscopy, and ion probe microanalysis. This text reflects the emphasis of the workshop on presenting the principles of analysis, the optimization of operating conditions, the description of successful techniques for sample preparation and quantitation, the illustration of problems and pitfalls, and the direction of microbeam analysis in biology.
Author: U. Lüttge
Publisher: Springer Science & Business Media
ISBN: 3642874967
Category : Science
Languages : en
Pages : 245
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Book Description
In biological literature, several definitions of quantitative autoradio graphy are given. The term is defined as either the determination and com parison of the density of silver grains above various structures or under varying conditions, or the determination of absolute quantities of radio activity. In both these cases, photometric measurement serves for more rapid and more exact evaluation of grain densities than would be possible by visual counting of the grains. The equipment generally used for the photometric measurement of silver grains consists of a microscope, a photocell, an electronic amplifier system and a display unit. Grains can be made accessible to photometric evaluation by various kinds of microscopic illumination: 1. Substage bright-field illumination. 2. Substage dark-field illumination. 3. Incident dark-field illumination. 4. Vertical bright-field illumination. With all these types of illumination, the relationship between the luminous flux I absorbed by the film, scattered into the objective and reflected or diffracted, and the flux 10 which is not affected by the film is used as a measure of grain density. Since these are differential measurements, the light beam I transmitted by the film is in itself a measure of grain density if the luminous flux 10 incident on the grains is kept constant. This approach has been used in a large number of measuring setups.
Author: Abraham Boekestein
Publisher: Springer Science & Business Media
ISBN: 3709166799
Category : Science
Languages : en
Pages : 271
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Book Description
This supplement of Mikrochimica Acta contains selected papers from the Second Workshop of the European Microbeam Analysis Society (EMAS) "Modern Developments and Applications in Microbeam Analysis", on which took place in May 1991 in Dubrovnik (Yugoslavia). EMAS was founded in 1987 by members from almost all European countries, in order to stimulate research, applications and development of all forms of microbeam methods. One of the most important activities EMAS is the organisation of biannual workshops for demonstrating the current status and developing trends of microbeam methods. For this meeting, EMAS chose to highlight the following topics: electron-beam microanalysis (EPMA) of thin films and quantitative analysis of ultra-light elements, Auger electron spectroscopy (AES), electron energy loss spec trometry (EELS), high-resolution transmission electron microscopy (HRTEM), quantitative analysis of biological samples and standard-less electron-beam microanalysis. Seven introductory lectures and almost seventy poster presentations were given by speakers from twelve European and two non-European (U.S.A. and Argentina) countries were made. One cannot assume that all fields of research in Europe were duly represented, but a definite trend is discernible. EPMA with wavelength-dispersive spectrometry (WDS) or energy-dispersive spectrometry (EDS) is the method with by far the widest range of applications, followed by TEM with EELS and then AES. There are also interesting suggestions for the further development of new appa ratus with new fields of application. Applications are heavily biased towards materials science (thin films in microelectronics and semicon ductors), ceramics and metallurgy, followed by analysis of biological and mineral samples.
