Microinjection and Organelle Transplantation Techniques PDF Download
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Author: Julio E. Celis
Publisher:
ISBN:
Category : Medical
Languages : en
Pages : 400
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Book Description
Microinjection of tissue culture cells using glass; Transformation capacity of SV40 DNA and subgenomic DNA fragments; Modulation of cellular activities via microinjection into living cells; Studies of retroviral packagin;. Erythrocyte-mediated transfer: methods; Erythrocyte-mediated transfer: applications; Liposomes for microinjection of molecules into cells; Liposomes in drug targeting; Liposomes as carriers for gene transfer in vivo; Transformation of mammalian cells with DNA using the calcium technique; The somatic cell as a tes tube: comparison between various transfer techniques.
Author: Julio E. Celis
Publisher:
ISBN:
Category : Medical
Languages : en
Pages : 400
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Book Description
Microinjection of tissue culture cells using glass; Transformation capacity of SV40 DNA and subgenomic DNA fragments; Modulation of cellular activities via microinjection into living cells; Studies of retroviral packagin;. Erythrocyte-mediated transfer: methods; Erythrocyte-mediated transfer: applications; Liposomes for microinjection of molecules into cells; Liposomes in drug targeting; Liposomes as carriers for gene transfer in vivo; Transformation of mammalian cells with DNA using the calcium technique; The somatic cell as a tes tube: comparison between various transfer techniques.
Author: Juan Carlos Lacal
Publisher: Birkhäuser
ISBN: 3034887051
Category : Science
Languages : en
Pages : 249
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Book Description
This manual describes the methodology and applications of microinjection, a powerful technique for transposing material into living cells through needle injection. Information is presented on the techniques principles, the required equipment, preparation of receiving cells and injected material, and the analysis of results. Detailed protocols for a wide range of applications in different cellular systems are included. A large part of the book is also dedicated to the Xenopus laevis oocyte system.
Author: Angel Cid-Arregui
Publisher: Springer Science & Business Media
ISBN: 3642803431
Category : Science
Languages : en
Pages : 619
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Book Description
The establishment of microinjection protocols about 20 years ago for cultured cells and shortly thereafter for the generation of transgenic mice by microinjection of DNA into fertilized mouse eggs greatly influ enced many fields of biology. Not only have the data generated using these approaches contributed to a large extent to our present under standing of gene regulation and cellular function of higher eukaryotic cells, but current knowledge and future developments in this area will certainly have a great impact on basic and applied research for many years to come. This laboratory manual describes the current state of the art in this research area and focuses primarily on both the experimental strategies with an extensive bibliography and the detailed procedures. A large number of studies are presently being performed and a great variety of new experimental designs are rapidly being developed. The book con tains protocols on injection of somatic cells as well as on injection of embryos, the use of similar equipment being a common feature. In the articles dedicated to somatic cells, full descriptions of the manual and automatic injection systems are given as well as the methods for the analysis of injected cells by video-microscopy, electron microscopy or in situ hybridizations. In addition, comprehensive protocols are given for injection experiments with very different purposes, such as to study sig nal transduction or microtubule dynamics.
Author: Nejat Düzgüneş
Publisher: Gulf Professional Publishing
ISBN: 9780121821227
Category : Membrane Fusion
Languages : en
Pages : 502
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Book Description
The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. * * Major topics covered include: * Cell-cell fusion mediated by viruses and viral proteins * Conformational changes of proteins during membrane fusion * Membrane fusion during exocytosis * Intracellular membrane fusion * Membrane fusion in fertilization * Introduction of macromolecules into cells by membrane fusion * Protoplast fusion.
Author: A.E. Sowers
Publisher: Springer Science & Business Media
ISBN: 147579598X
Category : Science
Languages : en
Pages : 545
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Book Description
Over the last decade the volume Membrane Fusion. edited by Poste and Nicholson, has probably served as one of the major sources of review in formation on fusion in membrane systems. Since its publication much new information has been collected. New methods of inducing fusion have been invented or discovered, and new applications for fusion have been found. The need for an up-to-date monograph that covers and in tegrates these subjects, reviews established material, and rationalizes and integrates the old and the new is thus obvious. This book is the product of efforts to meet this need. Most of the current work in the field of membrane fusion takes place within the context of intact or modified cells. Hence this book emphasizes the plasma membrane. Each chapter is either a review, a report, or a short historical overview, depending, respectively, on whether the subject is large in scope and has a long history, or the subject is in such an early stage of development that most of what is known is still in the hands of a relatively small number of investigators and is best covered in report form.
Author: Jan Wilschut
Publisher: CRC Press
ISBN: 1000723445
Category : Medical
Languages : en
Pages : 930
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Book Description
This balanced volume provides a broad and coherent overview of recent progress in membrane fusion research—highlighting an interdisciplinary treatment of the subject from the fields of biophysics, biochemistry, cell biology, virology, and biotechnology—in a single volume., Featuring easy-access sections on the general properties of membranes and applications of membrane fusion techniques, this valuable sourcebook outlines membrane structure, lipid polymorphism, and intermembrane forces ... covers membrane fusion in model systems ... presents the fusogenic properties of enveloped viruses ... discusses the fusion and flow of intracellular membranes and cell-cell fusion occurring during fertilization and myogenesis ... offers applications of membrane fusion techniques in cell-biological research and biotechnology ... and more. Supplying a comprehensive view of this exciting topic, Membrane Fusion is a working resource for molecular, cell, and membrane biologists; biophysicists; biochemists; virologists; biotechnologists; microbiologists; immunologists; physiologists; and graduate and medical school students in biophysics, biochemistry, physiology, virology, cell biology, and biotechnology.
Author: Robert A. Schlegel
Publisher: Elsevier
ISBN: 1483272702
Category : Science
Languages : en
Pages : 390
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Book Description
Molecular Regulation of Nuclear Events in Mitosis and Meiosis presents papers from researchers in various fields engaged in the scientific study of molecular mechanisms involved in the control of nuclear events in meiotic and mitotic cell activity. Various articles in the book discuss a wide range of topics such as the development of cytoplasmic activities that control chromosome cycles during maturation of amphibian oocytes; dynamics of the nuclear lamina during mitosis and meiosis; role of protein phosphorylation in xenopus oocyte meiotic maturation; and cell cycle studies of histone modifications. Molecular and cell biologists, oncologists, and biochemists will find the book invaluable.
Author: David R. Manning
Publisher: CRC Press
ISBN: 9781420048575
Category : Medical
Languages : en
Pages : 324
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Book Description
Incorporating a bench-top format, G Proteins: Techniques of Analysis covers essential methods - with a commitment to those techniques of proven and current utility. It offers an in-depth description of protocols, together with theory and representative data. It includes expression and functional analysis of G proteins; evaluation of covalent modifications and other regulatory phenomena; and, mapping pathways established among receptors, G proteins, and effectors. Incorporating contributors from key institutions, each contributor offers clear instructions to establish a synthesized, concise and consistent approach to each chapter, which is beneficial to both students and professionals.
Author:
Publisher: Academic Press
ISBN: 0080859291
Category : Science
Languages : en
Pages : 627
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Book Description
Vesicular Transport, Part A
Author: Bernard R. Glick
Publisher: CRC Press
ISBN: 1351091395
Category : Science
Languages : en
Pages : 384
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Book Description
Methods in Plant Molecular Biology and Biotechnology emphasizes a variety of well-tested methods in plant molecular biology and biotechnology. For each detailed and tested protocol presented, a brief overview of the methodology is provided. This overview considers why the protocol is used, what other comparable methods are available, and what limitations can be expected with the protocol. Other chapters in the book present overviews regarding how to approach particular problems and introduce unique methods - such as how to use computer methodology to study isolated genes. The book will be a practical reference for plant physiologists, plant molecular biologists, phytopathologists, and microbiologists.
