Methods of Protein Microcharacterization PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Methods of Protein Microcharacterization PDF full book. Access full book title Methods of Protein Microcharacterization by John E. Shively. Download full books in PDF and EPUB format.
Author: John E. Shively
Publisher: Springer Science & Business Media
ISBN: 1592594360
Category : Science
Languages : en
Pages : 458
Get Book Here
Book Description
Milestones in the techniques and methodology of polypeptide structure determination include the determination of the sequence of insulin by Sanger in 1951 (I) and the introduction of the repeti tive degradation of proteins with phenylisothiocyanate by Edman in 1959 (2). The automation of Edman chemistry (3) played a major role in the determination of polypeptide structures. Important modifications of Edman chemistry include the solid-phase approach by Laursen in 1971 (4) and the use of modified Edman reagents such as 4-N, N-dimethylaminoazobenzene-4'-isothiocy- ate (DABITC) for manual sequencing by Chang et al. (5) in 1976. A second major breakthrough in the analysis of polypeptides was automated amino acid analysis described by Spackman et al. in 1958 (6). However, during the period from 1975 to 1980, it became increasingly clear that the amount of material required for struc tural analysis was more than could be easily isolated for the vast majority of proteins. The field was criticized for its lack of sensitive techniques for the analysis of growth factors, immune modulators, membrane receptors, and peptide hormones. In addition, very little had been done to modernize and improve the original instruments introduced in the mid-1960s. The first indications of improved instrumentation for Edman chemistry came from Wittmann-Liebold's laboratory (7), followed by the introduction of a "micro" sequencer by Hunkapiller and Hood in 1978 (8). The movement toward improved instrumentation culminated in the "gas"--Phase sequencer of Hewick et al. (9) in 1981
Author: John E. Shively
Publisher: Springer Science & Business Media
ISBN: 1592594360
Category : Science
Languages : en
Pages : 458
Get Book Here
Book Description
Milestones in the techniques and methodology of polypeptide structure determination include the determination of the sequence of insulin by Sanger in 1951 (I) and the introduction of the repeti tive degradation of proteins with phenylisothiocyanate by Edman in 1959 (2). The automation of Edman chemistry (3) played a major role in the determination of polypeptide structures. Important modifications of Edman chemistry include the solid-phase approach by Laursen in 1971 (4) and the use of modified Edman reagents such as 4-N, N-dimethylaminoazobenzene-4'-isothiocy- ate (DABITC) for manual sequencing by Chang et al. (5) in 1976. A second major breakthrough in the analysis of polypeptides was automated amino acid analysis described by Spackman et al. in 1958 (6). However, during the period from 1975 to 1980, it became increasingly clear that the amount of material required for struc tural analysis was more than could be easily isolated for the vast majority of proteins. The field was criticized for its lack of sensitive techniques for the analysis of growth factors, immune modulators, membrane receptors, and peptide hormones. In addition, very little had been done to modernize and improve the original instruments introduced in the mid-1960s. The first indications of improved instrumentation for Edman chemistry came from Wittmann-Liebold's laboratory (7), followed by the introduction of a "micro" sequencer by Hunkapiller and Hood in 1978 (8). The movement toward improved instrumentation culminated in the "gas"--Phase sequencer of Hewick et al. (9) in 1981
Author: Roland Kellner
Publisher: John Wiley & Sons
ISBN: 3527615709
Category : Science
Languages : en
Pages : 284
Get Book Here
Book Description
Both a thorough introduction and laboratory guide! This book is based on the lectures and demonstrations presented at a successful workshop organized by the authors. Expert contributions and the support of more than a dozen companies engaged in bioanalysis and instrumentation enabled the participants to familiarize themselves with the most recent developments e.g. in protein separation and characterization (including laser desorption-ionisation mass spectrometry), fragmentation and micro sequencing. The workshop was held in the Max Planck Institute for Biochemistry at Martinsried. It helped graduate students and researchers from academia and industry both to understand and apply themselves the most sophisticated methods available. This book offers a stimulating combination of basic concepts and practical applications - and is thus useful at all levels.
Author: Roger L. Lundblad
Publisher: CRC Press
ISBN: 9780849326066
Category : Medical
Languages : en
Pages : 300
Get Book Here
Book Description
This exciting laboratory support book encompasses the encyclopedic coverage of the most frequently used methods for the site-specific chemical modification of proteins. In a concise manner, it presents methods for the characterization of modified proteins, including: amino acid analysis protein sequence analysis chemical cleavage of protein chains chromatographic separation of peptides It also discusses various approaches to the determination of solution protein concentration. It includes a complete literature survey of the various reagents, a list of the most commonly used reagents with their physical and chemical properties, and a list of preferred reagent suppliers.
Author: Antoine E. El-Khoury
Publisher: CRC Press
ISBN: 1351431935
Category : Medical
Languages : en
Pages : 276
Get Book Here
Book Description
Containing all the new as well as classical methodologies used in the investigation of amino acid and protein metabolism in human and animal models, this book is needed because of the dramatic increase in research in this field. There is no other book currently on the market that covers these methods of investigation. Methods for Investigation of Amino Acid and Protein Metabolism explores areas such as amino acid transfer across tissue membranes, past and new applications using stable isotopes, protein synthesis in organs and tissues, and more. Because of the importance of research methods in the field of amino acid and protein nutrition and metabolism, this book facilitates the reader's integration of the concepts involved in these investigative research methods and their corollaries. In addition to helping any nutrition investigator design and conduct appropriate research protocols in this area of nutrition, this book assists students who are planning to investigate amino acid and protein metabolism in humans or laboratory animals.
Author: Paul T. Matsudaira
Publisher: Elsevier
ISBN: 0080924611
Category : Science
Languages : en
Pages : 205
Get Book Here
Book Description
Why a Second Edition?The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. - Mass spectrophotometric characterization of proteins and peptides - N-terminal sequencing of proteins with blocked N termini - Internal amino acid sequence analysis after protease digestion in-gel and on-blot - Improved microscale peptide purification methods - Computer analysis of protein sequences - New protocols tested and refined through everyday use in authors' laboratories - Updated reference chapter covering all aspects of protein microsequencing
Author:
Publisher: Academic Press
ISBN: 0124735568
Category : Science
Languages : en
Pages : 555
Get Book Here
Book Description
Techniques in Protein Chemistry VII, a valuable bench-top reference tool for protein chemists, features the most up-to-date advances in protein methodologies. Key Features * Protein sequencing and amino acid analysis * Mass spectral analysis of peptides and proteins * Posttranslational processing * High-sensitivity protein and peptide separations * Protein folding and NMR * Functional domain analysis * Protein design and engineering
Author: M. Zouhair Atassi
Publisher: Springer Science & Business Media
ISBN: 148991031X
Category : Science
Languages : en
Pages : 522
Get Book Here
Book Description
The MPSA international conference is held in a different country every two years. It is devoted to methods of determining protein structure with emphasis on chemistry and sequence analysis. Until the ninth conference, MPSA was an acronym for Methods in Protein Sequence Analysis. To give the conference more flexibility and breadth, the Scientific Advisory Committee of the lOth MPSA decided to change the name to Methods in Protein Structure Analysis; however, the emphasis remains on "methods" and on "chemistry. " In fact, this is the only major conference that is devoted to methods. The MPSA conference is truly international, a fact clearly reflected by the composi tion of its Scientific Advisory Committee. The Scientific Advisory Committee oversees the scientific direction of the MPSA and elects the chairman of the conference. Members of the committee are elected by active members, based on scientific standing and activity. The chairman, subject to approval of the Scientific Advisory Committee, appoints the Organizing Committee. It is this latter committee that puts the conference together. The lectures of the MPSA have traditionally been published in a special proceedings issue. This is different from, and more detailed than, the special MPSA issue of the Journal of Protein Chemistry in which only a brief description of the talks is given in short papers and abstracts. In the I Oth MPSA, about half the talks are by invited speakers and the remainder were selected from submitted short papers and abstracts.
Author: Tony Hugli
Publisher: Elsevier
ISBN: 032314425X
Category : Science
Languages : en
Pages : 631
Get Book Here
Book Description
Techniques in Protein Chemistry compiles reports of methods and techniques presented at the second symposium of the Protein Society in August 1988. This book includes methods and applications in protein sequencing, advanced applications of mass spectrometry and nuclear magnetic resonance technology, limitations of amino acid microanalysis, and advances in high-performance liquid chromatography. The structure of synthetic test peptide-3 (STP-3), a peptide designed to test the analytical limits of current technology in the field of protein chemistry is also elaborated. This publication is suited for chemists and researchers conducting work on the analytical techniques available for the molecular characterization of proteins.
Author: Felix Franks
Publisher: Springer Science & Business Media
ISBN: 1592594387
Category : Science
Languages : en
Pages : 594
Get Book Here
Book Description
Proteins are the servants of life. They occur in all com- nent parts of living organisms and are staggering in their fu- tional variety, despite their chemical similarity. Even the simplest single-cell organism contains a thousand different p- teins, fulfilling a wide range of life-supporting roles. Additions to the total number of known proteins are being made on an increasing scale through the discovery of mutant strains or their production by genetic manipulation. The total international protein literature could fill a medi- sized building and is growing at an ever-increasing rate. The reader might be forgiven for asking whether yet another book on proteins, their properties, and functions can serve a useful purpose. An explanation of the origin of this book may serve as justification. The authors form the tutorial team for an int- sive postexperience course on protein characterization or- nized by the Center for Professional Advancement, East Brunswick, New Jersey, an educational foundation. The course was first mounted in Amsterdam in 1982 and has since been repeated several times, in both Amsterdam and the US, with participants from North America and most European countries. In a predecessor to this book, emphasis was placed on the role of protein isolation in the food industry, because at the time this reflected the interests of most of the participants at the course. Today, isolated proteins for food use are extracted from yeasts, fungal sources, legumes, oilseeds, cereals, and leaves.
Author: Roger L. Lundblad
Publisher: CRC Press
ISBN: 1439849005
Category : Medical
Languages : en
Pages : 422
Get Book Here
Book Description
Examining the chemical modification of biological polymers and the emerging applications of this technology, Chemical Modification of Biological Polymers reflects the change in emphasis in this subsection of biotechnology from the study of protein structure and function toward applications in therapeutics and diagnostics. HighlightsThe basic organi
