Author: Víctor M. Loyola-Vargas
Publisher: Springer Science & Business Media
ISBN: 1592599591
Category : Science
Languages : en
Pages : 389
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Book Description
A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.
Author: David W. Galbraith
Publisher: Academic Press
ISBN: 0125641516
Category : Medical
Languages : en
Pages : 605
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Book Description
Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. Key Features * Written by experts, many of whom have developed the individual methods described * Contains most, if not all, the methods needed for modern research in plant cell biology * Up-to-date and comprehensive * Full references * Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures * Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants.
Author:
Publisher: Elsevier
ISBN: 008085947X
Category : Science
Languages : en
Pages : 605
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Book Description
Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. Key Features* Written by experts, many of whom have developed the individual methods described* Contains most, if not all, the methods needed for modern research in plant cell biology* Up-to-date and comprehensive* Full references* Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures* Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants
Author:
Publisher: Academic Press
ISBN: 0080859488
Category : Science
Languages : en
Pages : 581
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Book Description
Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. This is accompanied by examples of typical results, illustrations, troubleshooting of potential pitfalls, sources of chemicals and equipment, and complete reference lists. Protocols are written to be easily comprehended by beginning research students, but these extensive volumes will also be a valuable addition to the libraries of expert researchers.Key Features* Written by experts, many of whom have developed the individual methods described* Contains most, if not all, the methods needed for modern research in plant cell biology* Up-to-date and comprehensive* Full references* Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures* Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants
Author: D. W. Galbraith
Publisher:
ISBN:
Category :
Languages : en
Pages : 573
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Book Description
Author: William V Dashek
Publisher: CRC Press
ISBN: 1482294494
Category : Science
Languages : en
Pages : 507
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Book Description
While there are a few plant cell biology books that are currently available, these are expensive, methods-oriented monographs. The present volume is a textbook for upper undergraduate and beginning graduate students. This textbook stresses concepts and is inquiry-oriented. To this end, there is extensive use of original research literature. As we live in an era of literature explosion, one must be selective. These judgements will naturally vary with each investigator. Input was sought from colleagues in deciding the literature to include. In addition to provision of select research literature, this volume presents citations and summaries of certain laboratory methods. In this connection, the textbook stresses quantitative data to enhance the student?s analytical abilities. Thus the volume contains computer-spread sheets and references to statistical packages, e.g. Harvard Graphics and Statistica.
Author: Julian Coleman
Publisher: Taylor & Francis
ISBN: 1000144712
Category : Nature
Languages : en
Pages : 212
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Book Description
Plant cell culture is an essential methodology in plant sciences, with numerous variant techniques depending on the cell type and organism. Plant Cell Culture provides the reader with a concise overview of these techniques, including basic plant biology for cell culture, basic sterile technique and media preparation, specific techniques for various plant cell and tissue types including applications, tissue culture in agriculture, horticulture and forestry and culture for genetic engineering and biotechnology. This book will be an essential addition to any plant science laboratory's bookshelf.
Author: Michael R. Davey
Publisher: John Wiley & Sons
ISBN: 9780470686515
Category : Science
Languages : en
Pages : 358
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Book Description
The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers.
Author: Víctor M. Loyola-Vargas
Publisher: Humana Press
ISBN: 9781617798177
Category : Science
Languages : en
Pages : 430
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Book Description
Cell culture methodologies have become standard procedures in most plant laboratories. Currently, facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. “Plant Cell Culture Protocols, Third Edition is divided into five convenient sections that cover topics from general methodologies, such as culture induction, growth and viability evaluation, statistical analysis and contamination control, to highly specialized techniques, such as clonal propagation, haploid production, somatic embryogenesis, organelle transformation. The volume concludes with a section on the laborious process of measuring the epigenetics changes in tissue cultures.”Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Cell Culture Protocols, Third Edition seeks to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.
Author: Arthur Weissbach
Publisher: Elsevier
ISBN: 1483269728
Category : Science
Languages : en
Pages : 564
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Book Description
Methods for Plant Molecular Biology is a collection of articles that focuses on the techniques used in plant molecular biology and genetics. The book discusses the isolation and characterization of nuclear, chloroplast, and mitochondrial nucleic acids and the factors and systems involved in transcription and gene expression. Procedures for the isolation of cell walls, chloroplast membranes, membrane proteins; techniques to carry out plant cell culture and protoplast formation; and methods for gene and organelle transfer are covered as well. Biologists, molecular biologists, botanists, and students will find the book very useful.
