Mass Spectrometry: Modified Proteins and Glycoconjugates PDF Download
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Author: A.L. Burlingame
Publisher: Gulf Professional Publishing
ISBN: 9780121828103
Category : Science
Languages : en
Pages : 482
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Book Description
This volume provides comprehensive treatment of tools and proper usage for the identification of proteins, affinity chromatography and studies the complexity of protein machines and assemblages, assignment of the most common protein posttranslational modifications (phosphorylation and glycosylation) and glycolipidomics. *Part 2 of 2 volumes about Mass Spectrometry *Discusses peptide and protein cleanup and preparation requirements for mass spectrometry *Explains protein enzymic and chemical digestion strategies *Includes case studies of protein assemblages and machines
Author: A.L. Burlingame
Publisher: Gulf Professional Publishing
ISBN: 9780121828103
Category : Science
Languages : en
Pages : 482
Get Book Here
Book Description
This volume provides comprehensive treatment of tools and proper usage for the identification of proteins, affinity chromatography and studies the complexity of protein machines and assemblages, assignment of the most common protein posttranslational modifications (phosphorylation and glycosylation) and glycolipidomics. *Part 2 of 2 volumes about Mass Spectrometry *Discusses peptide and protein cleanup and preparation requirements for mass spectrometry *Explains protein enzymic and chemical digestion strategies *Includes case studies of protein assemblages and machines
Author: John R. Griffiths
Publisher: John Wiley & Sons
ISBN: 1119045851
Category : Science
Languages : en
Pages : 414
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Book Description
Covers all major modifications, including phosphorylation, glycosylation, acetylation, ubiquitination, sulfonation and and glycation Discussion of the chemistry behind each modification, along with key methods and references Contributions from some of the leading researchers in the field A valuable reference source for all laboratories undertaking proteomics, mass spectrometry and post-translational modification research
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309260868
Category : Science
Languages : en
Pages : 171
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Book Description
A new focus on glycoscience, a field that explores the structures and functions of sugars, promises great advances in areas as diverse as medicine, energy generation, and materials science, this report finds. Glycans-also known as carbohydrates, saccharides, or simply as sugars-play central roles in many biological processes and have properties useful in an array of applications. However, glycans have received little attention from the research community due to a lack of tools to probe their often complex structures and properties. Transforming Glycoscience: A Roadmap for the Future presents a roadmap for transforming glycoscience from a field dominated by specialists to a widely studied and integrated discipline, which could lead to a more complete understanding of glycans and help solve key challenges in diverse fields.
Author: Claus-Wilhelm von der Lieth
Publisher: John Wiley & Sons
ISBN: 9780470029602
Category : Medical
Languages : en
Pages : 494
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Book Description
This book is the first to be dedicated to the bioinformatics of carbohydrates and glycoproteins. It provides an introduction to this emerging field of science both for the experimentalist working in glycobiology and glycomics, and also for the computer scientist looking for background information for the development of highly sophisticated algorithmic approaches. The book provides an overview of the state-of-the-art in the field, with reviews on databases, and the tools in use for analysis, interpretation, and prediction of the structures of complex carbohydrates, and demonstrates the value of bioinformatics for glycobiology. The availability of comprehensive databases and corresponding bioinformatics tools, to access and analyse the large amounts of experimental data relating to the structure of carbohydrates, will be a prerequisite for the success of the large-scale glycomics projects that aim to decipher new, so far unknown, biological functions of glycans. Efficient bioinformatics descriptions and tools can considerably enhance the efficiency of glycomics research, in terms of data quality, analysis and experimental costs. For a complete understanding of the molecular processes in which carbohydrates are involved, such as protein–carbohydrate interactions and the impact of glycosylation on protein function, knowledge of the 3D structure of the carbohydrate, the protein–carbohydrate complex, or the glycoprotein is often indispensable. This book provides a thorough introduction into methods used for conformational analysis of carbohydrates. Key features: Describes bioinformatic approaches to handle carbohydrate-active enzymes and glycosylation. Provides an overview on bioinformatics tools that facilitate analysis of carbohydrate structures. Gives introduction into molecular modelling of carbohydrate 3D structure and carbohydrates contained in the Protein Databank. Assumes only a basic knowledge of biology and bioinformatics.
Author: Lawrence C. Kuo
Publisher: Academic Press
ISBN: 0123812747
Category : Medical
Languages : en
Pages : 662
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Book Description
There are numerous excellent reviews on fragment-based drug discovery (FBDD), but there are to date no hand-holding guides or protocols with which one can embark on this orthogonal approach to complement traditional high throughput screening methodologies. This Methods in Enzymology volume offers the tools, practical approaches, and hit-to-lead examples on how to conduct FBDD screens. The chapters in this volume cover methods that have proven to be successful in generating leads from fragments, including chapters on how to apply computational techniques, nuclear magnetic resonance, surface plasma resonance, thermal shift and binding assays, protein crystallography, and medicinal chemistry in FBDD. Also elaborated by experienced researchers in FBDD are sample preparations of fragments, proteins, and GPCR as well as examples of how to generate leads from hits. Offers the tools, practical approaches, and hit-to-lead examples on how to conduct FBDD screens The chapters in this volume cover methods that have proven to be successful in generating leads from fragments, including chapters on how to apply computational techniques, nuclear magnetic resonance, surface plasma resonance, thermal shift and binding assays, protein crystallography, and medicinal chemistry in FBDD
Author: Christopher A. Voigt
Publisher: Academic Press
ISBN: 0123851203
Category : Bioengineering
Languages : en
Pages : 557
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Book Description
Synthetic biology encompasses a variety of different approaches, methodologies and disciplines and many different definitions exist. This volume covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation.
Author: James Whisstock
Publisher: Academic Press
ISBN: 0123864712
Category : Medical
Languages : en
Pages : 461
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Book Description
Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. The acronym serpin was originally coined because many serpins inhibit chymotrypsin-like serine proteases. This volume of Methods in Ezymology is split into 2 parts and comprehensively covers the subject.
Author: Helmut Sies
Publisher: Gulf Professional Publishing
ISBN: 9780121828059
Category : Science
Languages : en
Pages : 774
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Book Description
This volume on conjugation enzymes and transporters serves to bring together current methods and concepts in an interesting, important and rapidly developing field of cell and systems biology. Phase II Conjugation Enzymes and Transport Systems focuses on the so-called Phase II enzymes of drug metabolism (xenobiotics), which has important ramifications for endogenous metabolism and nutrition. Also included are aspects on Phase III, transport systems. This volume of Methods in Enzymology presents current knowledge and methodology on glucuronidation, sulfation, acetylation, and transport systems in this field of research. Together with the volumes on Quinones and Quinone Enzymes (volumes 378 and 382), and on Glutathione Transferases and gamma-Glutamyl Transpeptidases (volume 401), the state of knowledge on proteomics and metabolomics of many pathways of (waste) product elimination, enzyme protein induction and gene regulation and feedback control is provided. This volume will help stimulate future investigations and speed the advance of knowledge in systems biology. A laboratory standard for more than 40 years Over 400 volumes strong Also available on ScienceDirect
Author:
Publisher: Academic Press
ISBN: 0123859514
Category : Science
Languages : en
Pages : 562
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Book Description
Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. This volume in the Methods in Enzymology series comprehensively covers this topic. With an international board of authors, this volume covers subjects such as Crystallography of serpins and serpin complexes, Serpins as hormone transporters, and Production of serpins using cell free systems. - This volume in the Methods in Enzymology series comprehensively covers the topic of serpins - With an international board of authors, this volume covers subjects such as Crystallography of serpins and serpin complexes, Serpins as hormone transporters, and Production of serpins using cell free systems
Author:
Publisher: Academic Press
ISBN: 0123972299
Category : Science
Languages : en
Pages : 589
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Book Description
This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases. The contributions in this volume cover the broad scope of methods in the research on these enzymes. Several chapters describe quantitative biophysical and biochemical approaches to study molecular mechanisms and conformational changes of RNA helicases. Further chapters cover structural analysis, examination of co-factor effects on several representative examples, and the analysis of cellular functions of select enzymes. Two chapters outline approaches to the analysis of inhibitors that target RNA helicases. - This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases - The contributions in this volume cover the broad scope of methods in the research on these enzymes
