Laser Imaging and Manipulation in Cell Biology PDF Download
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Author: Francesco S. Pavone
Publisher: John Wiley & Sons
ISBN: 3527632255
Category : Science
Languages : en
Pages : 229
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Book Description
Here, the editor has gathered a team of international experts to present the latest advances in the field of laser imaging and manipulation techniques. The result is broad coverage of the interactions with biological samples to perform novel optical manipulation operations, both on the cellular and tissue levels. Of interest to physicists working and researching laser tissue mechanisms, cell biologists investigating new imaging and manipulation operation on the cellular level, medical doctors working with new laser therapies and diagnostic tools, as well as engineers developing new technologies in the field of optics and lasers.
Author: Francesco S. Pavone
Publisher: John Wiley & Sons
ISBN: 3527632255
Category : Science
Languages : en
Pages : 229
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Book Description
Here, the editor has gathered a team of international experts to present the latest advances in the field of laser imaging and manipulation techniques. The result is broad coverage of the interactions with biological samples to perform novel optical manipulation operations, both on the cellular and tissue levels. Of interest to physicists working and researching laser tissue mechanisms, cell biologists investigating new imaging and manipulation operation on the cellular level, medical doctors working with new laser therapies and diagnostic tools, as well as engineers developing new technologies in the field of optics and lasers.
Author: Douglas J. Taatjes
Publisher: Springer Science & Business Media
ISBN: 1592599931
Category : Medical
Languages : en
Pages : 505
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Book Description
A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.
Author:
Publisher: Academic Press
ISBN: 0080859534
Category : Science
Languages : en
Pages : 241
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Book Description
Volume 55 in Methods in Cell Biology is a concise laboratory book that emphasizes the methods and technologies needed to use single polarized laser light source that functions simultaneously as an optical trap and a dual-beam interferometer.* * Provides a practical laboratory guide for methods and technologies used with laser tweezers* Includes comprehensive and easy-to-follow protocols
Author: Francesco S. Pavone
Publisher: Wiley-VCH
ISBN: 9783527409297
Category : Science
Languages : en
Pages : 260
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Book Description
Here, the editor has gathered a team of international experts to present the latest advances in the field of laser imaging and manipulation techniques. The result is broad coverage of the interactions with biological samples to perform novel optical manipulation operations, both on the cellular and tissue levels. Of interest to physicists working and researching laser tissue mechanisms, cell biologists investigating new imaging and manipulation operation on the cellular level, medical doctors working with new laser therapies and diagnostic tools, as well as engineers developing new technologies in the field of optics and lasers.
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Book Description
Author: Robert D. Goldman
Publisher: CSHL Press
ISBN: 9780879696832
Category : Science
Languages : en
Pages : 658
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Book Description
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
Author: Arthur Ashkin
Publisher: World Scientific Publishing Company Incorporated
ISBN: 9789810240578
Category : Science
Languages : en
Pages : 915
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Book Description
This important volume contains selected papers and extensive commentaries on laser trapping and manipulation of neutral particles using radiation pressure forces. Such techniques apply to a variety of small particles, such as atoms, molecules, macroscopic dielectric particles, living cells, and organelles within cells. These optical methods have had a revolutionary impact on the fields of atomic and molecular physics, biophysics, and many aspects of nanotechnology.In atomic physics, the trapping and cooling of atoms down to nanokelvins and even picokelvin temperatures are possible. These are the lowest temperatures in the universe. This made possible the first demonstration of Bose-Einstein condensation of atomic and molecular vapors. Some of the applications are high precision atomic clocks, gyroscopes, the measurement of gravity, cryptology, atomic computers, cavity quantum electrodynamics and coherent atom lasers.A major application in biophysics is the study of the mechanical properties of the many types of motor molecules, mechanoenzymes, and other macromolecules responsible for the motion of organelles within cells and the locomotion of entire cells. Unique in vitro and in vivo assays study the driving forces, stepping motion, kinetics, and efficiency of these motors as they move along the cell's cytoskeleton. Positional and temporal resolutions have been achieved, making possible the study of RNA and DNA polymerases, as they undergo their various copying, backtracking, and error correcting functions on a single base pair basis.Many applications in nanotechnology involve particle and cell sorting, particle rotation, microfabrication of simple machines, microfluidics, and other micrometer devices. The number of applications continues to grow at a rapid rate.The author is the discoverer of optical trapping and optical tweezers. With his colleagues, he first demonstrated optical levitation, the trapping of atoms, and tweezer trapping and manipulation of living cells and biological particles.This is the only review volume covering the many fields of optical trapping and manipulation. The intention is to provide a selective guide to the literature and to teach how optical traps really work.
Author:
Publisher: Academic Press
ISBN: 0124202012
Category : Science
Languages : en
Pages : 609
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Book Description
This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material
Author: Pietro Ferraro
Publisher: Springer Science & Business Media
ISBN: 3642158137
Category : Science
Languages : en
Pages : 378
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Book Description
This book deals with the latest achievements in the field of optical coherent microscopy. While many other books exist on microscopy and imaging, this book provides a unique resource dedicated solely to this subject. Similarly, many books describe applications of holography, interferometry and speckle to metrology but do not focus on their use for microscopy. The coherent light microscopy reference provided here does not focus on the experimental mechanics of such techniques but instead is meant to provide a users manual to illustrate the strengths and capabilities of developing techniques. The areas of application of this technique are in biomedicine, medicine, life sciences, nanotechnology and materials sciences.
Author: Raquel Seruca
Publisher: CRC Press
ISBN: 1498737056
Category : Medical
Languages : en
Pages : 328
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Book Description
This comprehensive reference work details the latest developments in fluorescence imaging and related biological quantification. It explores the most recent techniques in this imaging technology through the utilization and incorporation of quantification analysis which makes this book unique. It also covers super resolution microscopy with the introduction of 3D imaging and high resolution fluorescence. Many of the chapter authors are world class experts in this medical imaging technology.
