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Author: Eva Harris
Publisher: Oxford University Press
ISBN: 0195119266
Category : Science
Languages : en
Pages : 327
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Book Description
The polymerase chain reaction (PCR) is a technique used to replicate specific pieces of DNA millions of times, which permits the detection and analysis of minute amounts of nucleic acids. Since its introduction in the late 1980s, this technique has been applied not only in molecular biology research but also in fields as diverse as anthropology, phylogeny, and forensics. However, despite the large impact of PCR, many of its applications remain within the confines of research and the academic environment. Now, in A Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques, Dr. Eva Harris makes this elegantly simple technique more accessible to researchers, physicians, and laboratory workers throughout the world. She provides a description of the theoretical basis of the technique, the practical details of the method, and the philosophy behind the technology transfer program that she developed over the last ten years. The book serves as a guide for potential users in developing countries and for scientists in developed countries who may wish to work abroad. In addition, the low-cost approach outlined in this book can be useful for high school, undergraduate, or continuing education programs in the United States. While the specific applications of PCR outlined in the book are immediately useful to the study of infectious diseases, the approach presented can be generalized to a number of other technologies and situations. The book will help laboratories in many areas of the world generate information on site for use by physicians, epidemiologists, public health workers, and health policy professionals to develop new strategies for disease control.
Author: Eva Harris
Publisher: Oxford University Press
ISBN: 0195119266
Category : Science
Languages : en
Pages : 327
Get Book Here
Book Description
The polymerase chain reaction (PCR) is a technique used to replicate specific pieces of DNA millions of times, which permits the detection and analysis of minute amounts of nucleic acids. Since its introduction in the late 1980s, this technique has been applied not only in molecular biology research but also in fields as diverse as anthropology, phylogeny, and forensics. However, despite the large impact of PCR, many of its applications remain within the confines of research and the academic environment. Now, in A Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques, Dr. Eva Harris makes this elegantly simple technique more accessible to researchers, physicians, and laboratory workers throughout the world. She provides a description of the theoretical basis of the technique, the practical details of the method, and the philosophy behind the technology transfer program that she developed over the last ten years. The book serves as a guide for potential users in developing countries and for scientists in developed countries who may wish to work abroad. In addition, the low-cost approach outlined in this book can be useful for high school, undergraduate, or continuing education programs in the United States. While the specific applications of PCR outlined in the book are immediately useful to the study of infectious diseases, the approach presented can be generalized to a number of other technologies and situations. The book will help laboratories in many areas of the world generate information on site for use by physicians, epidemiologists, public health workers, and health policy professionals to develop new strategies for disease control.
Author: Kristin Kimball
Publisher: Simon and Schuster
ISBN: 1416551611
Category : Biography & Autobiography
Languages : en
Pages : 320
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Book Description
After interviewing a young farmer, writer Kristen Kimball gave up her urban lifestyle to begin a farm with her interviewee near Lake Champlain in northern New York.
Author: Kristin Kimball
Publisher: Scribner
ISBN: 1501111531
Category : Biography & Autobiography
Languages : en
Pages : 304
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Book Description
From the celebrated author of the beloved bestseller The Dirty Life, a “beguiling memoir about the simple life” (Elle), Kristin Kimball describes the delicious highs and sometimes excruciating lows of life on Essex Farm—a 500-acre farm that produces a full diet for a community of 250 people. The Dirty Life chronicled Kimball’s move from New York City to 500 acres near Lake Champlain where she started a new farm with her partner, Mark. In Good Husbandry, she reveals what happened over the next five years at Essex Farm. Farming has many ups and downs, and the middle years were hard for the Kimballs. Mark got injured, the weather turned against them, and the farm faced financial pressures. Meanwhile, they had two small children to care for. How does one traverse the terrain of a maturing marriage and the transition from being a couple to being a family? How will the farm survive? What does a family need in order to be happy? Kristin had chosen Mark and farm life after having a good look around the world, with a fair understanding of what her choices meant. She knew she had traded the possibility of a steady paycheck, of wide open weekends and spontaneous vacations, for a life and work that was challenging but beautiful and fulfilling. So with grit and grace and a good sense of humor, she chose to dig in deeper. Featuring some of the same local characters and cherished animals first introduced in The Dirty Life, (Jet the farm dog, Delia the dairy cow, and those hardworking draft horses), plus a colorful cast of aspiring first-generation farmers who work at Essex Farm to acquire the skills they need to start sustainable farms of their own, Good Husbandry is about animals and plants, farmers and food, friends and neighbors, love and marriage, births and deaths, growth and abundance.
Author: Josh Kilmer-Purcell
Publisher: Harper Collins
ISBN: 006133698X
Category : Biography & Autobiography
Languages : en
Pages : 321
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Book Description
What happens when two New Yorkers (one an ex–drag queen) do the unthinkable: start over, have a herd of kids, and get a little dirty? Find out in this riotous and moving true tale of goats, mud, and a centuries-old mansion in rustic upstate New York—the new memoir by Josh Kilmer-Purcell, author of the New York Times bestseller I Am Not Myself These Days. A happy series of accidents and a doughnut-laden escape upstate take Josh and his partner, Brent, to the doorstep of the magnificent (and fabulously for sale) Beekman Mansion. One hour and one tour later, they have begun their transformation from uptight urbanites into the two-hundred-year-old-mansion-owning Beekman Boys. Suddenly, Josh—a full-time New Yorker with a successful advertising career—and Brent are weekend farmers, surrounded by nature's bounty and an eclectic cast: roosters who double as a wedding cover band; Bubby, the bionic cat; and a herd of eighty-eight goats, courtesy of their new caretaker, Farmer John. And soon, a fledgling business, born of a gift of handmade goat-milk soap, blossoms into a brand, Beekman 1802. The Bucolic Plague is tart and sweet, touching and laugh out loud funny, a story about approaching middle age, being in a long-term relationship, realizing the city no longer feeds you in the same way it used to, and finding new depths of love and commitment wherever you live.
Author: James R. Spotila
Publisher: JHU Press
ISBN: 142141709X
Category : Science
Languages : en
Pages : 246
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Book Description
The most comprehensive book ever written on leatherback sea turtles. Weighing as much as 2,000 pounds and reaching lengths of over seven feet, leatherback turtles are the world’s largest reptile. These unusual sea turtles have a thick, pliable shell that helps them to withstand great depths—they can swim more than one thousand meters below the surface in search of food. And what food source sustains these goliaths? Their diet consists almost exclusively of jellyfish, a meal they crisscross the oceans to find. Leatherbacks have been declining in recent decades, and some predict they will be gone by the end of this century. Why? Because of two primary factors: human redevelopment of nesting beaches and commercial fishing. There are only twenty-nine index beaches in the world where these turtles nest, and there is immense pressure to develop most of them into homes or resorts. At the same time, longline and gill net fisheries continue to overwhelm waters frequented by leatherbacks. In The Leatherback Turtle, James R. Spotila and Pilar Santidrián Tomillo bring together the world’s leading experts to produce a volume that reveals the biology of the leatherback while putting a spotlight on the conservation problems and solutions related to the species. The book leaves us with options: embark on the conservation strategy laid out within its pages and save one of nature’s most splendid creations, or watch yet another magnificent species disappear.
Author: Melissa Ursula Dawn Goldsmith
Publisher: Bloomsbury Publishing USA
ISBN:
Category : Social Science
Languages : en
Pages : 933
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Book Description
This set covers all aspects of international hip hop as expressed through music, art, fashion, dance, and political activity. Hip hop music has gone from being a marginalized genre in the late 1980s to the predominant style of music in America, the UK, Nigeria, South Africa, and other countries around the world. Hip Hop around the World includes more than 450 entries on global hip hop culture as it includes music, art, fashion, dance, social and cultural movements, organizations, and styles of hip hop. Virtually every country is represented in the text. Most of the entries focus on music styles and notable musicians and are unique in that they discuss the sound of various hip hop styles and musical artists' lyrical content, vocal delivery, vocal ranges, and more. Many additional entries deal with dance styles, such as breakdancing or b-boying/b-girling, popping/locking, clowning, and krumping, and cultural movements, such as black nationalism, Nation of Islam, Five Percent Nation, and Universal Zulu Nation. Country entries take into account politics, history, language, authenticity, and personal and community identification. Special care is taken to draw relationships between people and entities such as mentor-apprentice, producer-musician, and more.
Author: Jill Ker Conway
Publisher: Vintage
ISBN: 0307797309
Category : Biography & Autobiography
Languages : en
Pages : 258
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Book Description
In a memoir that pierces and delights us, Jill Ker Conway tells the story of her astonishing journey into adulthood—a journey that would ultimately span immense distances and encompass worlds, ideas, and ways of life that seem a century apart. She was seven before she ever saw another girl child. At eight, still too small to mount her horse unaided, she was galloping miles, alone, across Coorain, her parents' thirty thousand windswept, drought-haunted acres in the Australian outback, doing a "man's job" of helping herd the sheep because World War II had taken away the able-bodied men. She loved (and makes us see and feel) the vast unpeopled landscape, beautiful and hostile, whose uncertain weathers tormented the sheep ranchers with conflicting promises of riches and inescapable disaster. She adored (and makes us know) her large-visioned father and her strong, radiant mother, who had gone willingly with him into a pioneering life of loneliness and bone-breaking toil, who seemed miraculously to succeed in creating a warmly sheltering home in the harsh outback, and who, upon her husband's sudden death when Jill was ten, began to slide—bereft of the partnership of work and love that had so utterly fulfilled her—into depression and dependency. We see Jill, staggered by the loss of her father, catapulted to what seemed another planet—the suburban Sydney of the 1950s and its crowded, noisy, cliquish school life. Then the heady excitement of the University, but with it a yet more demanding course of lessons—Jill embracing new ideas, new possibilities, while at the same time trying to be mother to her mother and resenting it, escaping into drink, pulling herself back, striking a balance. We see her slowly gaining strength, coming into her own emotionally and intellectually and beginning the joyous love affair that gave wings to her newfound self. Worlds away from Coorain, in America, Jill Conway became a historian and the first woman president of Smith College. Her story of Coorain and the road from Coorain startles by its passion and evocative power, by its understanding of the ways in which a total, deep-rooted commitment to place—or to a dream—can at once liberate and imprison. It is a story of childhood as both Eden and anguish, and of growing up as a journey toward the difficult life of the free.
Author: Laurie Ruettimann
Publisher: Henry Holt and Company
ISBN: 1250269792
Category : Business & Economics
Languages : en
Pages : 240
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Book Description
"Indispensable reading for anyone seeking to improve their professional selves." —Daniel H. Pink, #1 New York Times bestselling author of When An essential guide for how to snap out of autopilot and become your own best advocate, with candid anecdotes and easy-to-adopt steps, from veteran HR specialist and popular podcast host Laurie Ruettimann Chances are you've spent the past few months cooped up inside, buried under a relentless news cycle and work that never seems to switch off. Millions of us worldwide are overworked, exhausted, and trying our hardest—yet not getting the recognition we deserve. It’s time for a fix. Top career coach and HR consultant Laurie Ruettimann knows firsthand that work can get a hell of a lot better. A decade ago, Ruettimann was uninspired, blaming others and herself for the unhappiness she felt. Until she had an epiphany: if she wanted a fulfilling existence, she couldn’t sit around and wait for change. She had to be her own leader. She had to truly take ahold of life—the good, the bad, and the downright ugly—in order to transform her future. Today, as businesses prioritize their bottom line over employee satisfaction and workers become increasingly isolated, the need to safeguard your well-being is crucial. And though this sounds intimidating, it’s easier to do than you think. Through tactical advice on how to approach work in a smart and healthy manner, which includes knowing when to sign off for the day, doubling down on our capacity to learn, fixing those finances, and beating impostor syndrome once and for all, Ruettimann lays out the framework necessary to champion your interests and create a life you actually enjoy. Packed with advice and stories of others who regained control of their lives, Betting on You is a game-changing must-read for how to radically improve your day-to-day, working more effectively and enthusiastically starting now.
Author: JoAnn Ermer-Seltun
Publisher: Lippincott Williams & Wilkins
ISBN: 1975164547
Category : Medical
Languages : en
Pages : 1359
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Book Description
Based on the curriculum blueprint of the Wound, Ostomy, and Continence Nursing Education Programs (WOCNEP) and approved by the Wound, Ostomy, and Continence Nurses SocietyTM (WOCN®), this practical text for continence care is your perfect source for expert guidance, training and wound, ostomy and continence (WOC) certification exam preparation. Full of expert advice, fundamental principles and vital clinical skills on continence care, Core Curriculum Continence Management, 2nd Edition is one of the few nursing texts to cover this practice area in detail.
Author: Lara Prior-Palmer
Publisher: Ebury Press
ISBN: 9781785038860
Category : Biography & Autobiography
Languages : en
Pages : 0
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Book Description
Lara Prior-Palmer was seeking the unknown. In search of adventure aged nineteen, she entered the world's toughest horse race - a 1000km. ride through extreme conditions in the Mongolian wilderness.
