Interaction Between Phage Functions and E. Coli RNA Polymerase in the Control of Transcription in PDF Download
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Author: Siobhan Yeats
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Siobhan Yeats
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Heeyoun Bunch
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Controlled gene expression in phage [lamda] is a model of transcription regulation which is mainly mediated by transcription factors to activate or repress E. coli RNA polymerase (RNAP). They switch appropriate genes on or off, determining the lysogenic or lytic pathway of progeny phages. Q is a [lamda] late gene regulator that exerts antitermination for products of the pR' promoter. Previous studies presented exciting aspects of [lamda]Q modification of RNAP which cause dramatic changes in the protein under certain conditions. RNAP engaged with [lamda]Q escapes a [sigma]-mediated promoter proximal pause and overcomes an intrinsic terminator downstream of the pR' promoter. In addition, [lamda]Q alters RNAP dynamics, aiding faster elongation, fewer pauses, and resistance to both the intrinsic and [rho]-dependent termination. Although [lamda]Q-mediated antitermination was characterized with previous works as described above, the mechanism by which [lamda]Q exerts antitermination is still unclear. In order to uncover this mechanism, this study attempted to answer the fundamental question of where [lamda]Q interacts with RNAP. First, the two largest subunits of RNAP were systematically dissected to map a [lamda]Q binding region on RNAP. The results suggested a clustered region of three fragments of [beta] and [beta]' for [lamda]Q binding, and as small as 82 amino acids ([beta]600-681) within this region were identified to bind to [lamda]Q. Second, a potential [lamda]Q binding region of RNAP proposed by a previous study was evaluated in this study. Although the region is located in the surface domain of [beta]600- 681, mutational analyses targeting the region demonstrated little [lamda]Q binding. Third, twelve RNAP mutants that reduce [lamda]Q-mediated antitermination were identified, and they suggested four spatial regions of RNAP as critical for [lamda]Q antitermination. Finally, one of the RNAP mutants, [beta]K1073G, presented altered function of NusA, a transcription elongation factor, in [lamda]Q-mediated antitermination at the intrinsic terminator, providing evidence of cooperation between NusA and [lamda]Q antitermination.
Author: Christopher K. Mathews
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 432
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Author: Won-Chul Suh
Publisher:
ISBN:
Category :
Languages : en
Pages : 430
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Author: Sigrid Leirmo
Publisher:
ISBN:
Category :
Languages : en
Pages : 324
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Author: Tao-shih Hsieh
Publisher:
ISBN:
Category :
Languages : en
Pages : 200
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Author: John Stuart Salstrom
Publisher:
ISBN:
Category :
Languages : en
Pages : 612
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![Interaction of E.coli RNA Polymerase with Phage [lambda] Promoters PDF](https://books.google.com/books/content/images/frontcover/vVsBGwAACAAJ?fife=w80-h100)
Author: Elizabeth Margaret Owens
Publisher:
ISBN:
Category : Bacteriophage
Languages : en
Pages : 172
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Author: Robert L. Ellis
Publisher: Elsevier
ISBN: 0080528880
Category : Science
Languages : en
Pages : 339
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The first of its kind, this volume presents the latest research findings on the chaperonins, the best studied family of a class of proteins known as molecular chaperones. These findings are changing our view of some fundamental cellular processes involving proteins, especially how proteins fold into their functional conformations. - Origins of the new view of protein folding - Prokaryotic chaperonins - Eukaryotic chaperonins - Evolution of the chaperonins - Refolding of denatured proteins - Organelle biosynthesis - Biomedical aspects
Author: E. C. C. Lin
Publisher: Springer
ISBN:
Category : Juvenile Nonfiction
Languages : en
Pages : 616
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Book Description
This up-to-date guide focuses on the understanding of key regulatory mechanisms governing gene expression in Escherichia coli. Studies of E. coli not only provide the first models of gene regulation, but research continues to yield different control mechanisms.
