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Author: Seyed Hesam Shahravan
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Seyed Hesam Shahravan
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Roza Maria Kamp
Publisher: Springer Science & Business Media
ISBN: 9783540202226
Category : Science
Languages : en
Pages : 448
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Following the succesful publication of "Proteome and Protein Analysis" in 2000, which was based on a former MPSA (Methods in Protein Structure Analysis) conference, "Methods in Proteome and Protein Analysis" presents the most interesting papers from the 14th MPSA meeting. Major topics include: X-ray crystallography, mass spectrometry or cryo-electron microscopy tomography and different experimental approaches for the study of very large multi-subunit molecular nanomaschines; development of high throughput methods for large-scale protein expression and purification and automatic data acquisition for structure determination by both X-ray diffraction and NMR spectroscopy; mechanisms of protein folding and misfolding in vitro and in vivo; protein-protein interactions; analysis of post-translational modifications; the classification, prediction of structure or functional sites, and evolution of protein folds and functions. TOC: Includes 25 chapters organized in the following parts: Structural Proteomics Proteome Analysis Structure-Function Correlations Protein-Protein Interaction Advanced Technologies Protein Sequencing and Amino Acids Analysis Bioinformatics
Author: Sharon A. Doyle
Publisher: Humana Press
ISBN: 9781603279758
Category : Science
Languages : en
Pages : 322
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Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields.
Author: Frances H. Arnold
Publisher: Springer Science & Business Media
ISBN: 1592593968
Category : Science
Languages : en
Pages : 381
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Directed evolution comprises two distinct steps that are typically applied in an iterative fashion: (1) generating molecular diversity and (2) finding among the ensemble of mutant sequences those proteins that perform the desired fu- tion according to the specified criteria. In many ways, the second step is the most challenging. No matter how cleverly designed or diverse the starting library, without an effective screening strategy the ability to isolate useful clones is severely diminished. The best screens are (1) high throughput, to increase the likelihood that useful clones will be found; (2) sufficiently sen- tive (i. e. , good signal to noise) to allow the isolation of lower activity clones early in evolution; (3) sufficiently reproducible to allow one to find small improvements; (4) robust, which means that the signal afforded by active clones is not dependent on difficult-to-control environmental variables; and, most importantly, (5) sensitive to the desired function. Regarding this last point, almost anyone who has attempted a directed evolution experiment has learned firsthand the truth of the dictum “you get what you screen for. ” The protocols in Directed Enzyme Evolution describe a series of detailed p- cedures of proven utility for directed evolution purposes. The volume begins with several selection strategies for enzyme evolution and continues with assay methods that can be used to screen enzyme libraries. Genetic selections offer the advantage that functional proteins can be isolated from very large libraries s- ply by growing a population of cells under selective conditions.
Author: Grant D. Schauer
Publisher:
ISBN:
Category : Molecules
Languages : en
Pages : 244
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Author: Allan Matte
Publisher: Elsevier
ISBN: 0081030193
Category : Business & Economics
Languages : en
Pages : 222
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Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics provides the interested and informed reader with an overview of current approaches, strategies and considerations relating to the purification, analytics and characterization of therapeutic antibodies and related molecules. While there are obviously other books published in and around this subject area, they seem to be either older (c.a. year 2000 publication date) or are more limited in scope. The book will include an extensive bibliography of the published literature in the respective areas covered. It is not, however, intended to be a how-to methods book. Covers the vital new area of R&D on therapeutic antibodies Written by leading scientists and researchers Up-to-date coverage and includes a detailed bibliography
Author: Huimin Zhao
Publisher: John Wiley & Sons
ISBN: 3527344705
Category : Science
Languages : en
Pages : 41
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A one-stop reference that reviews protein design strategies to applications in industrial and medical biotechnology Protein Engineering: Tools and Applications is a comprehensive resource that offers a systematic and comprehensive review of the most recent advances in the field, and contains detailed information on the methodologies and strategies behind these approaches. The authors—noted experts on the topic—explore the distinctive advantages and disadvantages of the presented methodologies and strategies in a targeted and focused manner that allows for the adaptation and implementation of the strategies for new applications. The book contains information on the directed evolution, rational design, and semi-rational design of proteins and offers a review of the most recent applications in industrial and medical biotechnology. This important book: Covers technologies and methodologies used in protein engineering Includes the strategies behind the approaches, designed to help with the adaptation and implementation of these strategies for new applications Offers a comprehensive and thorough treatment of protein engineering from primary strategies to applications in industrial and medical biotechnology Presents cutting edge advances in the continuously evolving field of protein engineering Written for students and professionals of bioengineering, biotechnology, biochemistry, Protein Engineering: Tools and Applications offers an essential resource to the design strategies in protein engineering and reviews recent applications.
Author: National Academies of Sciences, Engineering, and Medicine
Publisher: National Academies Press
ISBN: 0309676738
Category : Science
Languages : en
Pages : 201
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One of the holy grails in biology is the ability to predict functional characteristics from an organism's genetic sequence. Despite decades of research since the first sequencing of an organism in 1995, scientists still do not understand exactly how the information in genes is converted into an organism's phenotype, its physical characteristics. Functional genomics attempts to make use of the vast wealth of data from "-omics" screens and projects to describe gene and protein functions and interactions. A February 2020 workshop was held to determine research needs to advance the field of functional genomics over the next 10-20 years. Speakers and participants discussed goals, strategies, and technical needs to allow functional genomics to contribute to the advancement of basic knowledge and its applications that would benefit society. This publication summarizes the presentations and discussions from the workshop.
Author: Bob Chen
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Over the past decade, high-throughput technologies have allowed researchers to gain unprecedented insights into intrinsically complex and interconnected biological systems. As examples, whole-genome sequencing has enabled the identification of crucial genes and mutations underlying disease pathophysiology, DNA microarrays have been used to elucidate transcription patterns involved in healthy and diseased states, and large-scale proteomics methods have helped map the connectivity of cell signaling networks that orchestrate responses to growth factors and other external stimuli. In contrast, analogously powerful approaches for rapidly and deeply interrogating the sequence-structure-activity relationship of proteins, with functional read-outs that span a range of biophysical and biochemical measurements, have lagged because of technical challenges. In this dissertation, I describe the development of a new technology platform, termed μSCALE (microcapillary single-cell analysis and laser extraction), that aims to improve high-throughput protein characterization and engineering. The μSCALE platform is built around a microcapillary array, automated fluorescence imaging, and a novel laser-based sample recovery method. The platform spatially segregates millions of cells or their protein products in a microcapillary array and interrogates the dense array within minutes. μSCALE also enables recovery of target sample from the microcapillary array using a precise laser-based extraction technique. To showcase capabilities and breadth of μSCALE, I applied it to three distinct protein-analysis and engineering applications, using libraries expressed in yeast or bacteria. In each case, I demonstrated feasibility and then engineered a novel protein variant, identifying new binders against a clinical target, creating a novel fluorescent protein biosensor, or reducing inhibition of an enzyme. Compared to conventional screening techniques, such as FACS and plate-based screens, the μSCALE affords several distinctions advantages, including a five orders of magnitude improvement in throughput, direct cell imaging, and high sorting purity and yield. Collectively, these benefits present μSCALE as an exciting, novel technology to the field of protein engineering and beyond.
Author: Renaud Vincentelli
Publisher: Methods in Molecular Biology
ISBN: 9781493996261
Category : Science
Languages : en
Pages : 537
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