Principles and Techniques of Biochemistry and Molecular Biology PDF Download
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Author: Keith Wilson
Publisher: Cambridge University Press
ISBN: 0521516358
Category : Medical
Languages : en
Pages : 769
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Book Description
Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Author: Keith Wilson
Publisher: Cambridge University Press
ISBN: 0521516358
Category : Medical
Languages : en
Pages : 769
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Book Description
Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Author: R. J. Mayer
Publisher:
ISBN:
Category : Cytochemistry
Languages : en
Pages : 348
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Book Description
Author: Simon Renshaw
Publisher: John Wiley & Sons
ISBN: 1118717775
Category : Science
Languages : en
Pages : 258
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Book Description
Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount. It is with this fundamental aim in mind that we have compiled Immunohistochemistry: Essential Methods. We have achieved this by examining each aspect of immunochemistry in turn, with each chapter including detailed information regarding the subject matter in question. Each chapter is written by an expert in their field and includes protocols that are typically used in their own research. Subjects covered are, amongst others, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. In addition, benefits and limitations of each approach are discussed within the chapters.
Author: Heddy Zola
Publisher: CRC Press
ISBN:
Category : Medical
Languages : en
Pages : 288
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Book Description
This two-volume reference details immunological techniques for biologists of all disciplines. Volume I includes a detailed discussion of the tissue culture laboratory. It addresses what the lab needs to be, and the general "housekeeping" procedures involved in tissue culture. Presented next are chapters on specific aspects of tissue culture and hybridoma technology. The book includes a review of bioassays for interleukins, and a series of papers on lymphokines and functional assays in vitro. The section on molecular genetic studies begins with consideration of the choice of strategies for cloning the genes of cell surface molecules. It continues with papers on aspects of molecular biological techniques most closely related to immunology. The final section covers immunochemical techniques. Volume II reviews techniques used with small laboratory animals. It includes papers on specialized procedures with animals. Technical aspects are emphasized through a detailed analysis of effects of ultraviolet light on the immune system. Covered also is antigen detection in cells and tissue. The book addresses the important areas of protein purification using monoclonal antibodies.These two volumes are of great importance to those who use immunological techniques, whether they are immunologists or trained in other disciplines. The book is intended for those in animal science, veterinary science, genetics and cell biology, bacteriology, immunology, pathology, biochemistry, laboratory medicine, and hematology.
Author: Eric G. Berger
Publisher: Springer Science & Business Media
ISBN: 9783764356927
Category : Golgi apparatus
Languages : en
Pages : 328
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Book Description
In 1898 Camillo Golgi reported his newly observed intracellular structure, the apparato reticolare interno, now universally known as the Golgi Apparatus. The method he used was an ingenious histological technique (La reazione nera) which brought him fame for the discovery of neuronal networks and culminated in the award of the Nobel Prize for Physiology and Medicine in 1906. This technique, however, was not easily reproducible and led to a long-lasting controversy about the reality of the Golgi apparatus. Its identification as a ubiquitous organelle by electron microscopy turned out to be the breakthrough and incited an enormous wave of interest in this organelle at the end of the sixties. In recent years immunochemical techniques and molecular cloning approaches opened up new avenues and led to an ongoing resurgence of interest. The role of the Golgi apparatus in modifying, broadening and refining the structural information conferred by transcription/translation is now generally accepted but still incompletely understood. During the coming years, this topic certainly will remain center stage in the field of cell biology. The centennial of the discovery of this fascinating organelle prompted us to edit a new comprehensive book on the Golgi apparatus whose complexity necessitated the contributions of leading specialists in this field. This book is aimed at a broad readership of glycobiologists as well as cell and molecular biologists and may also be interesting for advanced students of biology and life sciences.
Author: William H. Yong
Publisher: Humana Press
ISBN: 9781493989331
Category : Medical
Languages : en
Pages : 470
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Book Description
This volume not only discusses various common biobanking topics, it also delves into less-discussed subjects such as what is needed to start a biobank, training of new biobanking personnel, and ethnic representation in biospecimen research. Other chapters in this book span practical topics including: disaster prevention and recovery; information technology; flora and fauna preservation including zoological fluid specimen photography; surgical and autopsy biobanking; biobanking of bodily fluids; biosafety; cutting frozen sections; immunohistochemistry; nucleic acid extraction; and biospecimen shipping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and comprehensive, Biobanking: Methods and Protocols is a valuable resource for novice and practicing biobankers, and for end-user researchers. This book aims to bring new insight into the field and expand on current biomedical biobanking studies.
Author: Loriano Ballarin
Publisher: Academic Press
ISBN: 0128032537
Category : Medical
Languages : en
Pages : 326
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Book Description
Lessons in Immunity: From Single-cell Organisms to Mammals stems from the activity of the Italian Association of Developmental and Comparative Immunobiology (IADCI), represented by the editors. This book is presented as a series of short overviews that report on the current state of various relevant fields of immunobiology from an evolutionary perspective. The overviews are written by authors directly involved in the research, and most are members of the IADCI or have otherwise been involved in the related research for their respective overview. This publication offers scientists and teachers an easy and updated reference tool. - Provides simple and updated reviews on the immunobiology of a wide spectrum of organisms, considered in an evolutionary context - Focuses on both cells and humoral components of a variety of non-classical model organisms - Offers in a single volume many contributions which can help with understanding the evolution of immune responses and the main adaptations in animal phyla - Presents a valuable holistic cross-sectional approach for teaching immunology and its applications
Author: Andreas Hofmann
Publisher: Cambridge University Press
ISBN: 1108365256
Category : Science
Languages : en
Pages : 962
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Book Description
Bringing this best-selling textbook right up to date, the new edition uniquely integrates the theories and methods that drive the fields of biology, biotechnology and medicine, comprehensively covering both the techniques students will encounter in lab classes and those that underpin current key advances and discoveries. The contents have been updated to include both traditional and cutting-edge techniques most commonly used in current life science research. Emphasis is placed on understanding the theory behind the techniques, as well as analysis of the resulting data. New chapters cover proteomics, genomics, metabolomics, bioinformatics, as well as data analysis and visualisation. Using accessible language to describe concepts and methods, and with a wealth of new in-text worked examples to challenge students' understanding, this textbook provides an essential guide to the key techniques used in current bioscience research.
Author: S. V. S. Rana
Publisher: Rastogi Publications
ISBN: 9788171338863
Category : Biochemistry
Languages : en
Pages : 336
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Book Description
Author: Harald Tschesche
Publisher: Walter de Gruyter
ISBN: 3110252368
Category : Science
Languages : en
Pages : 379
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Book Description
This book presents a survey of recent developments in protein biochemistry. Top researchers in the field of protein biochemistry describe modern methods to address the challenges of protein purification by three-phase partitioning, and their folding and degradation by the functions of chaperones. The significance of peptide purity for fibril formation is addressed as well as the use of target oriented peptide arrays in palliative approaches in mucoviszidose. The design and application of protein epitope mimetics just as the structural resolving of the misfolding of various mutant proteins in serpinopathies enlarge our tools in resolving pathophysiological imbalances.
