Imaging and Spectroscopic Analysis of Living Cells Optical and Spectroscopic Techniques PDF Download
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Author: P. Michael Conn
Publisher:
ISBN:
Category :
Languages : en
Pages : 506
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Book Description
Author: P. Michael Conn
Publisher:
ISBN:
Category :
Languages : en
Pages : 506
Get Book
Book Description
Author: P. Michael Conn
Publisher: Academic Press
ISBN: 0123884489
Category : Medical
Languages : en
Pages : 594
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Book Description
This volume of Methods in Enzymology is the second of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. Expert authors who are leaders in the field Extensively referenced and useful figures and tables Provides hints and tricks to facilitate reproduction of methods
Author: P. Michael Conn
Publisher:
ISBN:
Category :
Languages : en
Pages : 506
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Book Description
Going back to the dawn of light microscopy, imaging techniques have provided the opportunity for developing models of cellular function. Over the past 40 years, the availability of technology for high-resolution imaging and for evaluation of those images in live cells has extended our reach and, accordingly, our ability to understand cell function. Given the large number of choices in equipment and approaches, sorting out the best approach can be challenging, even to seasoned investigators. The present volumes provide descriptions of methods used to image living cells, with particular reference to the technical approaches and reagents needed and approaches to selecting the best techniques. The authors explain how these methods are able to provide important biological insights in normal and pathological cells. Authors were selected based on research contributions in the area about which they have written and based on their ability to describe their methodological contribution in a clear and reproducible way. They have been encouraged to make use of graphics, comparisons to other methods, and to provide tricks and approaches not revealed in prior publications that make it possible to adapt methods to other systems
Author: P. Michael Conn
Publisher: Academic Press
ISBN: 0123918561
Category : Cell physiology
Languages : en
Pages : 559
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Book Description
This volume of Methods in Enzymology is the third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. Publisher's note.
Author:
Publisher: Academic Press
ISBN: 9780080859286
Category : Science
Languages : en
Pages : 502
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Book Description
Fluorescence Microscopy of Living Cells in Culture, Part B
Author:
Publisher: Academic Press
ISBN: 0123918693
Category : Science
Languages : en
Pages : 560
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Book Description
This volume of Methods in Enzymology is the first of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. Expert authors who are leaders in the field Extensively referenced and useful figures and tables Provides hints and tricks to facilitate reproduction of methods
Author: Vicente Micó
Publisher: Frontiers Media SA
ISBN: 288971523X
Category : Science
Languages : en
Pages : 221
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Book Description
Author: Wlodek M. Bujalowski
Publisher: Humana Press
ISBN: 9781493961412
Category : Medical
Languages : en
Pages : 397
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Book Description
Quantitative elucidation of structural, energetic and dynamic aspects of macromolecular interactions is indispensable for understanding the functional activities of biomolecules and their interactions. The optical spectroscopic methods are not confined to small molecules or macromolecules but permit the studies of even the largest biological systems in their full splendor, including the living cell. In, Spectroscopic Methods of Analysis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study properties of individual macromolecules, their complexes, organelles, and cells, using optical spectroscopic techniques. These include methods and approaches for experimental and theoretical analyses of fluorescence properties of the examined systems, single molecule approaches, electronic absorption, and electro-optical analyses of macromolecular interactions, structures, and dynamics. Written in the highly successful Methods in Molecular Biology(tm) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Spectroscopic Methods of Analysis : Methods and Protocols seeks to aid scientists in the further study of optical spectroscopic methods.
Author: Jay L. Nadeau
Publisher: CRC Press
ISBN: 1317974522
Category : Medical
Languages : en
Pages : 791
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Book Description
Praise for the First Edition “essential reading for any physical scientist who is interested in performing biological research.” ―Contemporary Physics "an ambitious text.... Each chapter contains protocols and the conceptual reasoning behind them, which is often useful to physicists performing biological experiments for the first time." –Physics Today This fully updated and expanded text is the best starting point for any student or researcher in the physical sciences to gain firm grounding in the techniques employed in molecular biophysics and quantitative biology. It includes brand new chapters on gene expression techniques, advanced techniques in biological light microscopy (super-resolution, two-photon, and fluorescence lifetime imaging), holography, and gold nanoparticles used in medicine. The author shares invaluable practical tips and insider’s knowledge to simplify potentially confusing techniques. The reader is guided through easy-to-follow examples carried out from start to finish with practical tips and insider’s knowledge. The emphasis is on building comfort with getting hands "wet" with basic methods and finally understanding when and how to apply or adapt them to address different questions. Jay L. Nadeau is a scientific researcher and head of the Biomedical Engineering in Advanced Applications of Quantum, Oscillatory, and Nanotechnological Systems (BEAAQONS) lab at Caltech and was previously associate professor of biomedical engineering and physics at McGill University.
Author:
Publisher: Academic Press
ISBN: 012420094X
Category : Science
Languages : en
Pages : 408
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Book Description
Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols In this volume we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate
