Basic Techniques in Molecular Biology PDF Download
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Author: Stefan Surzycki
Publisher: Springer Science & Business Media
ISBN: 3642569684
Category : Science
Languages : en
Pages : 442
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Book Description
This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Author: Stefan Surzycki
Publisher: Springer Science & Business Media
ISBN: 3642569684
Category : Science
Languages : en
Pages : 442
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Book Description
This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Author: M.L.M. Anderson
Publisher: Garland Science
ISBN: 1000144550
Category : Science
Languages : en
Pages : 279
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Book Description
First published in 1998. This book is targeted at the beginner who has little or no knowledge of the principles or practice of hybridization. This text concentrates on solution and filter hybridization with a final chapter on current developments which includes DNA chips and advances in probe design.
Author: P. Tijssen
Publisher: Elsevier
ISBN: 0080858937
Category : Science
Languages : en
Pages : 361
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Book Description
Recent breakthroughs in recombinant DNA technology and the availability of sophisticated equipment accessible to almost any laboratory, have contributed to the development and perfection of powerful hybridization tools. Recently, nucleic acid hybridization has not only become a cornerstone in molecular biology research but also a powerful supplement to other diagnostic tools.These diagnostic methods are set out in a logical and clear two-part volume in this now famous Laboratory Techniques series. The volume is divided into theory and preparation (Part I), and probe labelling and hybridization techniques (Part II). Both parts are worthy additions to this series, designed for easy access of information on the laboratory bench.
Author: Anton Buzdin
Publisher: Springer Science & Business Media
ISBN: 1402060408
Category : Science
Languages : en
Pages : 328
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Book Description
Several approaches, such as microarray hybridization, have become extremely popular tools for specialists in biochemistry and biomedicine, while the potential of many other advantageous techniques seems to be underestimated. Written by an international team of authors, this book details the current state-of-art in hybridization techniques. Coverage includes experimental protocols along with comprehensive and detailed method descriptions.
Author: P. Tijssen
Publisher: Elsevier Science & Technology
ISBN:
Category : Science
Languages : en
Pages : 336
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Book Description
Recent breakthroughs in recombinant DNA technology and the availability of sophisticated equipment accessible to almost any laboratory, have contributed to the development and perfection of powerful hybridization tools. Recently, nucleic acid hybridization has not only become a cornerstone in molecular biology research but also a powerful supplement to other diagnostic tools. These diagnostic methods are set out in a logical and clear two-part volume in this now famous Laboratory Techniques series. The volume is divided into theory and preparation (Part I), and probe labelling and hybridization techniques (Part II). Both parts are worthy additions to this series, designed for easy access of information on the laboratory bench.
Author: Friedhelm Hildebrandt
Publisher: Springer Science & Business Media
ISBN: 3642598110
Category : Science
Languages : en
Pages : 394
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Book Description
This manual not only provides reliable, up-to-date protocols for lab use but also the theoretical background of molecular biology, allowing users to better understand the principles underlying these techniques. It covers a wide range of methods, including the purification of nucleic acids, enzymatic modification of DNA, isolation of specific DNA fragments, PCR, cloning techniques, and gene expression. A Springer Lab Manual
Author: Thomas Liehr
Publisher: Academic Press
ISBN: 0128235802
Category : Science
Languages : en
Pages : 430
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Book Description
Cytogenomics demonstrates that chromosomes are crucial in understanding the human genome and that new high-throughput approaches are central to advancing cytogenetics in the 21st century. After an introduction to (molecular) cytogenetics, being the basic of all cytogenomic research, this book highlights the strengths and newfound advantages of cytogenomic research methods and technologies, enabling researchers to jump-start their own projects and more effectively gather and interpret chromosomal data. Methods discussed include banding and molecular cytogenetics, molecular combing, molecular karyotyping, next-generation sequencing, epigenetic study approaches, optical mapping/karyomapping, and CRISPR-cas9 applications for cytogenomics. The book's second half demonstrates recent applications of cytogenomic techniques, such as characterizing 3D chromosome structure across different tissue types and insights into multilayer organization of chromosomes, role of repetitive elements and noncoding RNAs in human genome, studies in topologically associated domains, interchromosomal interactions, and chromoanagenesis. This book is an important reference source for researchers, students, basic and translational scientists, and clinicians in the areas of human genetics, genomics, reproductive medicine, gynecology, obstetrics, internal medicine, oncology, bioinformatics, medical genetics, and prenatal testing, as well as genetic counselors, clinical laboratory geneticists, bioethicists, and fertility specialists. - Offers applied approaches empowering a new generation of cytogenomic research using a balanced combination of classical and advanced technologies - Provides a framework for interpreting chromosome structure and how this affects the functioning of the genome in health and disease - Features chapter contributions from international leaders in the field
Author: Gerard Morel
Publisher: CRC Press
ISBN: 1000611396
Category : Science
Languages : en
Pages : 634
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Book Description
In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization
Author: Ralph Rapley
Publisher: Springer Science & Business Media
ISBN: 1592590381
Category : Science
Languages : en
Pages : 997
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Book Description
A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."
Author: Chung Eun Ha
Publisher: Academic Press
ISBN: 0080916880
Category : Science
Languages : en
Pages : 600
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Book Description
Expert biochemist N.V. Bhagavan's new work condenses his successful Medical Biochemistry texts along with numerous case studies, to act as an extensive review and reference guide for both students and experts alike. The research-driven content includes four-color illustrations throughout to develop an understanding of the events and processes that are occurring at both the molecular and macrolecular levels of physiologic regulation, clinical effects, and interactions. Using thorough introductions, end of chapter reviews, fact-filled tables, and related multiple-choice questions, Bhagavan provides the reader with the most condensed yet detailed biochemistry overview available. More than a quick survey, this comprehensive text includes USMLE sample exams from Bhagavan himself, a previous coauthor. - Clinical focus emphasizing relevant physiologic and pathophysiologic biochemical concepts - Interactive multiple-choice questions to prep for USMLE exams - Clinical case studies for understanding basic science, diagnosis, and treatment of human diseases - Instructional overview figures, flowcharts, and tables to enhance understanding
